The Identification of a Novel Spider Toxin Peptide, Lycotoxin-Pa2a, with Antibacterial and Anti-Inflammatory Activities
Abstract
:1. Introduction
2. Results
2.1. Identification of a Novel Toxin Peptide from the Transccriptome of Spider Pardosa astrigera
2.2. Antibacterial Activity of Lytx-Pa2a against Pathogenic Bacteria
2.3. Bacterial Membrane Permeation and ROS Production by Lytx-Pa2a
2.4. Effects of Lytx-Pa2a on Mammalian Cells
2.5. Inhibition of LPS-Induced Inflammation by Lytx-Pa2a
3. Discussion
4. Materials and Methods
4.1. In Silico Analysis of Peptide Sequence
4.2. Bacterial Strains and Cell Lines
4.3. Antimicrobial Activity Assay
4.4. Intrabacterial ROS Detection
4.5. Cell Viablility Assay and LDH-Release Assay
4.6. Hemolytic Activity Measurement
4.7. NO Quatification
4.8. RT-qPCR
4.9. Western Blot Analysis
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Antimicrobial Activity | Anti-Inflammatory Activity | Hemolytic Activity | |||
---|---|---|---|---|---|
ADAM | sAMPred-GAT | AmpGram | PreAIP | PreTP-Stack | DBAASP 1 |
0.7 (AMP) | AMP | 0.9894 | 0.589 (AIP) | AIP | Not active |
MIC (μM) | E. coli | P. aeruginosa | B. cereus | S. aureus |
---|---|---|---|---|
Lytx-Pa2a | 1 | 1 | 1 | 8 |
Melittin | 1 | 8 | 1 | 2 |
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Shin, M.K.; Hwang, I.-W.; Jang, B.-Y.; Bu, K.-B.; Han, D.-H.; Lee, S.-H.; Oh, J.W.; Yoo, J.S.; Sung, J.-S. The Identification of a Novel Spider Toxin Peptide, Lycotoxin-Pa2a, with Antibacterial and Anti-Inflammatory Activities. Antibiotics 2023, 12, 1708. https://doi.org/10.3390/antibiotics12121708
Shin MK, Hwang I-W, Jang B-Y, Bu K-B, Han D-H, Lee S-H, Oh JW, Yoo JS, Sung J-S. The Identification of a Novel Spider Toxin Peptide, Lycotoxin-Pa2a, with Antibacterial and Anti-Inflammatory Activities. Antibiotics. 2023; 12(12):1708. https://doi.org/10.3390/antibiotics12121708
Chicago/Turabian StyleShin, Min Kyoung, In-Wook Hwang, Bo-Young Jang, Kyung-Bin Bu, Dong-Hee Han, Seung-Ho Lee, Jin Wook Oh, Jung Sun Yoo, and Jung-Suk Sung. 2023. "The Identification of a Novel Spider Toxin Peptide, Lycotoxin-Pa2a, with Antibacterial and Anti-Inflammatory Activities" Antibiotics 12, no. 12: 1708. https://doi.org/10.3390/antibiotics12121708