Characterization of Two Novel AmpC Beta-Lactamases from the Emerging Opportunistic Pathogen, Cedecea neteri
Abstract
:1. Introduction
2. Results
2.1. Sequence Analysis of AmpC Proteins
2.2. Overexpression and Purification of rAmpC Proteins
2.3. Determination of Extended-Spectrum AmpC β-Lactamase (ESAC) and Class B Metallo-β-Lactamase Activity
2.4. Enzymatic Activity and Kinetic Analysis of the rAmpC Proteins
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains, Chemicals, and Supplies
4.2. Cloning of the AmpC Genes
4.3. Expression and Purification of AmpC Proteins
4.4. Kinetic Analysis of the rAmpC Proteins
4.5. Assay for Extended-Spectrum AmpC β-Lactamase (ESAC) and Class B Metallo-β-Lactamase Activity
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Strain | Km (µM) | kcat (s−1) | kcat/Km (mM−1s−1) |
---|---|---|---|
E. cloacae (ATCC 13047) | 11 ± 2 | 0.83 ± 0.04 | 7.5 |
C. neteri (SSMD04) | 46 ± 5 | 1.93 ± 0.16 | 41.9 |
C. neteri (ATCC 33855) | 133 ± 11 | 3.13 ± 0.27 | 23.5 |
Km,apparent (µM) 1 | |||
---|---|---|---|
ATCC 13047 | SSMD04 | ATCC 33855 | |
Antibiotic | |||
Ampicillin | 7 | 72 | 12 |
Penicillin | 33 | 58 | 11 |
Cefazolin | 0.43 | 67 | 100 |
Cephalexin | 6.8 | 2 | 5 |
Cefoxitin | 12 | 48 | 54 |
Cefuroxime | 0.35 | 5 | 4 |
Imipenem | 0.85 | 5 | 2 |
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Sharkady, S.M.; Bailey, B.; Thompson, D.K. Characterization of Two Novel AmpC Beta-Lactamases from the Emerging Opportunistic Pathogen, Cedecea neteri. Antibiotics 2023, 12, 219. https://doi.org/10.3390/antibiotics12020219
Sharkady SM, Bailey B, Thompson DK. Characterization of Two Novel AmpC Beta-Lactamases from the Emerging Opportunistic Pathogen, Cedecea neteri. Antibiotics. 2023; 12(2):219. https://doi.org/10.3390/antibiotics12020219
Chicago/Turabian StyleSharkady, Stephen M., Brandon Bailey, and Dorothea K. Thompson. 2023. "Characterization of Two Novel AmpC Beta-Lactamases from the Emerging Opportunistic Pathogen, Cedecea neteri" Antibiotics 12, no. 2: 219. https://doi.org/10.3390/antibiotics12020219