Green Synthesis of Silver Nanoparticle from Anadenanthera colubrina Extract and Its Antimicrobial Action against ESKAPEE Group Bacteria
Abstract
:1. Introduction
2. Results
2.1. Synthesis of Biogenic Nanoparticles
2.2. Average Diameter Size, Zeta Potential and Polydispersity Index (PDI)
2.3. Fourier Transform Infrared Spectroscopy (FT-IR)
2.4. X-ray Diffraction (XRD)
2.5. Electron Microscopy Images
2.6. Antibacterial Activity
2.7. Hemolytic Activity
3. Discussion
4. Materials and Methods
4.1. Preparation of the Extract
4.2. Biosynthesis of Bio-AgNPs
4.3. Characterization of Bio-AgNPs
4.4. Evaluation of the Biological Activity of Bio-AgNPs
4.4.1. Tested Strains
4.4.2. Antibacterial Activity
4.4.3. Hemolytic Activity Assay of Bio-AgNPs
5. Conclusions
6. Patents
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Tested Bacteria | MIC (µM) | MBC (µM) | AgNO3 MIC |
---|---|---|---|
Enterococcus faecium 700 | 39.06 | 156.25 | 31.25 |
Staphylococcus aureus ATCC 25923 | 78.12 | 156.25 | 62.5 |
Staphylococcus aureus BEC 9393 | 78.12 | 156.25 | 62.5 |
Klebsiella pneumoniae 3978 | 39.06 | 39.06 | 31.25 |
Acinetobacter baumannii 141 | ≤19.53 | ≤19.53 | 7.81 |
Pseudomonas aeruginosa 3167 | ≤19.53 | ≤19.53 | 7.81 |
Enterobacter cloacae 9434 | ≤19.53 | ≤19.53 | 31.25 |
Escherichia coli ATCC 25922 | 78.12 | 78.12 | 15.62 |
Escherichia coli ATCC 8739 | 78.12 | 156.25 | 15.62 |
Escherichia coli 5616 | 39.06 | 78.12 | 15.62 |
Bacterial Isolates | Resistance Profile |
---|---|
Enterococcus faecium 700 | Vancomycin-resistant |
Staphylococcus aureus BEC 9393 | Methicillin-resistant |
Klebsiella pneumoniae 3978 | Carbapenem-resistant * |
Acinetobacter baumannii 141 | Carbapenem-resistant * |
Pseudomonas aeruginosa 3167 | Carbapenem-resistant * |
Enterobacter cloacae 9434 | Carbapenem-resistant * |
Escherichia coli 5616 | Carbapenem-resistant * |
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Deonas, A.N.; Souza, L.M.d.S.; Andrade, G.J.S.; Germiniani-Cardozo, J.; Dahmer, D.; de Oliveira, A.G.; Nakazato, G.; Torezan, J.M.D.; Kobayashi, R.K.T. Green Synthesis of Silver Nanoparticle from Anadenanthera colubrina Extract and Its Antimicrobial Action against ESKAPEE Group Bacteria. Antibiotics 2024, 13, 777. https://doi.org/10.3390/antibiotics13080777
Deonas AN, Souza LMdS, Andrade GJS, Germiniani-Cardozo J, Dahmer D, de Oliveira AG, Nakazato G, Torezan JMD, Kobayashi RKT. Green Synthesis of Silver Nanoparticle from Anadenanthera colubrina Extract and Its Antimicrobial Action against ESKAPEE Group Bacteria. Antibiotics. 2024; 13(8):777. https://doi.org/10.3390/antibiotics13080777
Chicago/Turabian StyleDeonas, Anastácia Nikolaos, Lucas Marcelino dos Santos Souza, Gabriel Jonathan Sousa Andrade, Jennifer Germiniani-Cardozo, Débora Dahmer, Admilton Gonçalves de Oliveira, Gerson Nakazato, José Marcelo Domingues Torezan, and Renata Katsuko Takayama Kobayashi. 2024. "Green Synthesis of Silver Nanoparticle from Anadenanthera colubrina Extract and Its Antimicrobial Action against ESKAPEE Group Bacteria" Antibiotics 13, no. 8: 777. https://doi.org/10.3390/antibiotics13080777