Persistence and Culturability of Escherichia coli under Induced Toxin Expression
Abstract
:1. Introduction
2. Results
2.1. Dynamic Changes in Persistence and Culturability under hipA Expression
2.2. Dynamic Changes in Culturability and Dormancy under hipA Induction
2.3. Dynamic Changes in Cell Counts Revealed More Information about Dormancy
2.4. Arabinose-Induced Expression of hipA
2.5. Corroborating the Results Using a Growth Reporter Strain
3. Discussion
4. Materials and Methods
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Primer Sequence |
---|---|
rrsA | FP: 5′-ctcttgccatcggatgtgccca-3′ RP: 5′-cagtgtggctggtcatcctctca-3′ |
hipA | FP: 5′-ggcggtacgggaatacacat-3′ RP: 5′-ccgggaatctcagcaccttt-3′ |
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Dhaouadi, Y.; Hashemi, M.J.; Ren, D. Persistence and Culturability of Escherichia coli under Induced Toxin Expression. Antibiotics 2024, 13, 863. https://doi.org/10.3390/antibiotics13090863
Dhaouadi Y, Hashemi MJ, Ren D. Persistence and Culturability of Escherichia coli under Induced Toxin Expression. Antibiotics. 2024; 13(9):863. https://doi.org/10.3390/antibiotics13090863
Chicago/Turabian StyleDhaouadi, Yousr, Mohamad Javad Hashemi, and Dacheng Ren. 2024. "Persistence and Culturability of Escherichia coli under Induced Toxin Expression" Antibiotics 13, no. 9: 863. https://doi.org/10.3390/antibiotics13090863