1. Introduction
One of the most significant factors for success of any fixed dental prosthetic restoration is the accuracy of shade matching with the surrounding dentition [
1]. The importance of accurate color matching is further increased when restoring anterior teeth [
2]. Metal ceramic restorations are widely used because they provide composite of both strength and esthetic [
2,
3]. Multiple features of restorations such as shade, shape, and texture play an instrumental role in giving a natural appearance [
4]. Duplicating the exact shade of that of surrounding teeth is more challenging in the case of metal ceramic restorations due to the dark-colored metal underlying the translucent porcelain [
2,
5,
6,
7]. Factors other than the underlying metal that make the color matching more demanding include adequate tooth preparation, proper selection of appropriate shade in clinics, different brands of porcelain requiring variation in condensation techniques, translucency, fluorescence, opacity, thickness of porcelain layer, surface characteristics, time, and temperature of firing porcelain [
2,
4,
5,
7].
To attain best possible esthetics, the duplication of shades of porcelain is performed in two phases, shade selection followed by shade duplication [
4]. The first step is performed in the clinic and the second is a challenge for the laboratory (lab) [
4,
5]. In dental clinics, the shade selection is dependent on multiple factors such as observer, light source, surroundings such as makeup, color of walls, gloves, cleanliness of teeth, duration and time of shade selection, distance from teeth, and level of eyes of observer when noting the shade [
8]. The techniques for color matching are broadly categorized into instrumental or visual analysis [
3,
9]. Many methods are available for shade assessment such as objective analysis by spectrophotometer, three-dimensional guide, digital shade scanner, image analysis technique, and colorimeter [
4,
8]. Presently, the use of digital cameras for noting the shade and communicating it to the lab is also being practiced [
4]. After ensuring the accuracy of clinical technique for shade matching, effective and clear communication between clinic and laboratory personnel also plays an influential role in accurate shade duplication [
9].
The precision in color matching or reproduction in the final product may vary from natural teeth or even shade tab due to various reasons in the laboratory [
4]. The actual cause of the variation in color is still undetermined [
2]. The possible reason for variations in shades may be associated with various factors: optical changes and thickness of opaque layer after firing of porcelain, effect of metal oxides, different type of metal copings used underneath porcelain, and skills of the technician [
2,
10]. The change in color may also be due to use of shade guide of different brand from the brand of porcelain used in the laboratory [
4,
10]. In addition, changes in the surface texture due to glazing, the technique sensitivity, variability, and complexity in procedures to fabricate a restoration further add to the risk factors of increasing errors [
6].
With recent advances in prosthodontics regarding techniques, materials and technology, shade matching, and reproduction has been improvised [
4,
10]. The CIE LAB system aids in detailed measurement of shade as well as helps in recognizing any difference in value, chroma, or hue of the shade [
2,
4]. Still, there is room for improvement in replication of accurate shade of restoration and further evaluation of factors leading to the difference in shades when restorations are fabricated in different laboratories. This knowledge needs the realization that different shade production from color matching appointment is still an issue and needs to be addressed. Even though the dental professionals have been mindful of the fact that there are variations between shade tab and shade reproduced on the restorations since long ago, recently the need to improve this issue has been arising due to more awareness through media about esthetics and pressure to improve appearance or smile as well as the improving standard of the dental specialists and higher expectations from the patients [
3,
10]. Objective measurement of shade using the instrumental methods is considered more accurate compared to subjective analysis of shade [
8]. Spectrophotometry and the CIE LAB system aid in objective evaluation of the shade [
8]. Thus, the present study utilized a spectrophotometer for evaluation of shade with the aim to analyze the differences in color of metal ceramic restorations fabricated from different laboratories.
2. Materials and Methods
The research was carried out at the Department of Prosthodontics and CDRC, College of Dentistry, King Saud University, Riyadh, KSA.
2.1. Calculation of Sample Size for Each Shade and Total Sample Size
Six different laboratories were requested to prepare the samples of metal ceramic discs, ten samples of shade A1, ten of shade B1, and ten of shade C1. Each laboratory provided 30 samples, and total samples from all labs were 180 (N = 180). For the power analysis of the sample size, G-power software (G* Power 3.1.9.7, Germany) was used, keeping in view the sample sizes used in some of the previously reported research. The total sample size calculated was to be 180 specimens; 30 specimens each for the six dental laboratories included in the study. The analysis was carried out with the effect size (0.5), power (0.82), and the level of significance (p < 0.05).
2.2. Sample Fabrication
The samples for test groups were prepared in the shape of discs with standard dimensions of 2 mm thickness and 10 mm diameter, as described previously [
2]. No special instructions were given to the dental laboratories for using a specific brand of metal ceramic material. However, the dental laboratories were instructed to follow the manufacturers’ recommendations for fabrication of the specimens. The sequence used for fabrication of discs involved fabrication of the metal part of the disc followed by porcelain powder application over the metal in three phases, i.e., wash opaque, followed by opaque, and lastly build up porcelain. The specimens were prepared by skilled technicians and two specialists assessed the prepared metal ceramic discs (
Figure 1). Thickness was standardized and confirmed by using an electronic caliper device [
2].
2.3. Thermocycling of Samples
To imitate the clinical condition or condition in oral cavity, the prepared samples were kept in distilled water at temperature of 37 °C for 24 h. Samples were then subjected to process of ageing by keeping the sample in a thermocycling machine (Huber, SD-Mechatronik-Thermocycler, Westerham, Germany) between temperatures 5 °C and 55 °C for 6000 rotations with a 30 s dwell time and a 5 s transfer time [
11].
2.4. Color Analysis of Samples
A spectrophotometer (LabScan-XE
®, Hunter-Lab, Sunset Hills Road, Reston, VA, USA) was used for measurements of color coordinates of all the test samples. This device is considered to be a reliable tool for determining the tooth shade and is considered the gold standard for the research studies related to tooth color [
9,
10,
11]. Each prepared sample was positioned over the spectrophotometer sensor, and three observations for every sample were noted. The instrument sent light waves into the test specimens and the light waves either refracted or reflected back to the receiver were quantified as L*a*b* color coordinates based on the opponent color theory assuming that receptors in the human eye perceive color as the following pairs of opposites. The “L” value for each scale indicated the level of light/dark, the “a” value redness/greenness, and the “b” value yellowness/blueness. All three values were obtained for complete description of the specimen’s color. Stability of color was evaluated by calculating delta E (ΔE) using L*a*b* values using the below mentioned formula [
9,
10,
11]:
2.5. Statistical Analysis
All the data was compiled and analyzed using Statistical Package for Social Sciences (SPSS; Version 23; SPSS Inc., Chicago, IL, USA). The mean values of L*a*b* color coordinates, calculation of ΔE from the L*a*b* values for each sample of the metal ceramic disks, were included in descriptive variables. The data were found to be normally distributed according to Kolmogorov–Smirnov test. The statistical analysis included comparisons of ΔE mean values with confidence interval of 95% for all samples from all labs using one-way analysis of variance (ANOVA) and Tukey’s post hoc tests for multiple comparisons. The level of significance was set at α < 0.05.
3. Results
A total sample of N = 180 porcelain fused to metal discs were collected from six labs. Each lab provided 10 samples (30 from each lab) for each shade, i.e., A1, B1, and C1. Each shade contributed equally (33.33%; 60/180) to the sample size.
Statistics for descriptive variables (standard deviation (S.D.) and mean) of values for L*a*b* were noted with the help of the spectrophotometer for all the samples (N = 180) as presented in
Table 1,
Table 2 and
Table 3. The minimum value for L* (lightness) was 67.98 ± 1.32 in shade A1 (lab 1), and maximum value for L* was 79.85 ± 0.12 in shade C1 (lab 2). The value for a* (redness–greenness) was minimum −2.2 ± 0.09 and maximum 1.19 ± 0.27 in shade B1 (lab 5) and A1 (lab 1), respectively. The value for b* (yellowness and blueness) was minimum 4.75 ± 0.18 and maximum 14.75 ± 0.51 in shade B1 (lab 1) and C1 (lab 4), respectively, as shown in
Table 1,
Table 2 and
Table 3.
The delta E calculated for each shade is also shown in
Table 1,
Table 2 and
Table 3. The P value for all shades was 0.000, which was highly significant. This validates that there is a difference between the similar shades when obtained by different labs. The maximum value for ΔE in shade A1 was 77.0 ± 0.065 by lab 5 and minimum value for ΔE noted for shade A1 was 69.19 ± 1.39 by lab 1. The values of ΔE for shade B1 were maximum 80.14 ± 0.16 for lab 5 and minimum 68.38 ± 0.40 for lab 1, and the values of ΔE for shade C1 were maximum 80.16 ± 0.14 by lab 2 and minimum 71.62 ± 1.23 by lab 4, respectively.
The multiple comparisons of ΔE as analyzed by post hoc Tukey HSD test are presented in
Table 4. Majority values showed statistically significant results. The mean variation between delta E values for shade A1 among different labs showed statistically significant difference in comparison between all labs except between labs 1 and 4, labs 2 and 5, and labs 3 and 6. The mean variation between delta E values for shade B1 among different labs showed statistically significant difference in comparison between all labs except between lab 4 and 6, and for shade C, all values were significant except the comparison between lab 1 with 3 and 4, lab 2 with 5, and lab 3 with 4, respectively, as shown in
Table 4. Twelve out of 15 comparisons for shade A1, 14 out of 15 comparisons for shade B1, and 11 out of 15 comparisons for shade C1 showed significant difference in shade matching. Thus, different labs showed variations in shade reproduction.
4. Discussion
The present study conducted the in vitro analysis of shades of metal ceramic restoration samples prepared by different laboratories. The difference in shade of samples made by different labs was estimated using a digital spectrophotometer and CIE LAB system. The objective analysis of color of metal ceramic restoration by spectrophotometer is not influenced by variations in light source or observer [
8,
12,
13]. The reliability and repeatability of this technique is universally accepted as it aids in precise measurement of color of restoration [
13,
14,
15,
16]. It evaluates the absorption of light at different wavelengths [
13,
14,
15]. The technique sensitivity and expensive armamentarium of the spectrophotometer limits its frequent use in clinical settings [
14]. Ideally, the shade duplication from all labs should exhibit no color differences and must be standardized. Multiple factors lead to variations in color replication [
1]. The slight difference in color replication of the same shade by different labs can be explained as an overlapping area between black and white, i.e., grey zone [
1]. Thus, exact shade match should be given a range or minor margin of error instead of a definite value [
1].
The current study reported statistically significant difference in the same shades produced by different labs. This dismisses the null hypothesis of no variation in color duplication by different labs. The results of this study are in accordance with another study by Omar et al., who reported a significant difference in porcelain fused to metal crown shades when evaluated by a spectrophotometer [
17]. The present study also has similar results to the research by Douglas et al., where a significant difference was observed in the shade duplication among different labs [
1]. The similarity in these studies may be due to use of the same technique for evaluation in terms of objective methods of color analysis. The study provided detailed instructions for standardization at each step, thus reducing the chance of error [
1].
A study by Sam et al. found a significant difference in a* values but the difference was insignificant in L* and b* values [
7]. They finally concluded that although insignificant for some values and significant for a*, the variation was observed in all values [
7]. This might be due to the differences in the sample size, as Sam et al. used only 40 crowns for observation. In addition, the mode of analysis of shade was digital pictures and visual methods [
7]. This can be the reason for difference in significance of results. In addition, different parts of crown emit different wavelengths leading to variations in the optical conditions [
15]. This, in addition to different lighting conditions while taking digital photographs, might have led to differences in the results.
The findings of the current study are partially in contrast to research by Bin-Shuwaish et al., who reported that shade matching was good for cervical and middle third [
4]. Although this study also found that there was a difference in shade duplication in incisal third, this is in conformity with the present study [
4]. The partial difference might be due to smaller sample size of this study, and the partial similarity of this study might be due to the use of instrumental techniques to assess shade matching or use of appropriate aperture size of the colorimeter to reduce the chances of error due to edge loss [
4]. The other reasons for differences in results of different studies may be attributed to factors such as technique sensitivity of porcelain application, changes due to different number of firing cycles, glazing, thickness of porcelain in crowns, or use of different modes for assessment of shade, i.e., objective versus subjective analysis [
12,
18,
19]. The differences or similarities may also be due to use of different or similar brands of porcelain or difference in equipment owned by different labs [
4]. The variations may also be due to observation of different areas of curved crown as compared to analysis of flat surfaces of discs, as in the case of the present study [
18,
20]. According to the literature, the color changes in dental materials have been divided into three categories [
17]. The ΔE values greater than 3.7 depict a very poor shade match, and ΔE value of more than 2 depicts clinically unacceptable color match. The ΔE value of less than 1 means that color difference is not perceptible with eyes [
6,
17,
21,
22,
23]. Even though the results of the present study demonstrated significant findings for shade variation, the values fall under the third category, which means that color difference is not evident to unaided sight; thus, slight variation is acceptable clinically.
Although great effort was made to ensure standardization, some limitations are still unavoidable. There is always a margin for human error in preparation of samples or observation of reading [
4]. Other limitations of the study were that single porcelain brand was not specified to be used by all labs, which might have resulted in difference of shades due to difference in brands. Disc-shaped samples should have been compared with discs, or proper crowns should have been formed for more accurate analysis. Since this was in vitro analysis, the sample observed was dry, whereas for in vivo, the crown surface is wet. For future studies, it is recommended to include, assess, and compare parameters related to the materials, processing technologies, and surface finishing/glazing. Furthermore, using a thin layer of saliva or salivary simulant when performing in vitro analysis will be valuable.
5. Conclusions
The investigation demonstrated the following:
Similar tooth shades (A1, B1, and C1) when duplicated by different labs exhibited highly significant color variations.
According to ΔE calculations for each tested shade (A1, B1, and C1), significant variations were observed in shade duplication.
To avoid or limit the variations in shade matching, standardization methods should be considered, such as similar approaches for shade selection, and digital technology, rather than manual shade selection, should be used to reduce the human errors. Similarly, processing parameters including types of porcelain, fabrication technique, and firing cycles should be standardized. All the laboratory technical staff should be trained following the same curriculum and techniques for similarity in shade reproduction.