Current Progress and Open Challenges for Combined Toxic Effects of Manufactured Nano-Sized Objects (MNO’s) on Soil Biota and Microbial Community
, Ammarah Hasnain 1,2, Jie Wang 1,*,†, Hanxian Zheng 1, Syed Atif Hasan Naqvi 3,*, Ram Prasad 4, Ateeq ur Rehman 3, Muhammad Amir Sohail 5, Muhammad Zeeshan Hassan 3, Muhammad Farhan 3, Muhammad Altaf Khan 6 and Mahmoud Moustafa 7,8
Round 1
Reviewer 1 Report
I want to suggest to the authors some recommendations trying to improve the article. First of all, I want to express my interest on the paper because I find this issue of great importance.
In the abstract, when using first-time MNOs (line 35), it is desirable t add previously manufactured nano-size objects to understand the meaning. After that, it is easy to understand this abbreviation along the text. I suggest to review in part the English style in the abstract.
The introduction gives enough information updated but, in my opinion, we have to distinguished the natural nano-size objects from those manufactured because their role and origin is very different. It seems not so clear in the text. Do authors considered soil microaggregates as nano-size objects with functions similar to those of the MNOs?
In several lines, try to avoid cutting the word at the end of the sentence in such way that makes it easy to read, if possible. For instance, lines 53-54, “devel-opment” maybe “deve-lopment”; and others like in line 74-75 “nan-ofibers”.
In line 107, please write correctly “Ca+2”.
Line 157, please use the citation instead of this sentence: “according to the authors of the reference [24]”
The position of tables and figures would be arranged to avoid have them cut between sheets. Congrats, the figures are so nice and interesting.
In general, I think that the article can be published and in my opinion, it is a review of great interest.
Author Response
Point by Point Response to Reviewer-I Comments and Suggestions for Authors
I want to suggest to the authors some recommendations trying to improve the article. First of all, I want to express my interest on the paper because I find this issue of great importance.
Response: Thank you very much for your encouraging remarks. The suggested directions have been implemented in the whole manuscript in order to improve the quality of the article.
In the abstract, when using first-time MNOs (line 35), it is desirable to add previously manufactured nano-size objects to understand the meaning. After that, it is easy to understand this abbreviation along the text.
Response: Thank you for your valuable suggestions, the correction has been made in the light of direction as the manufactured nano-size objects has been written once and then in the complete article MNO’s has been written.
I suggest to review in part the English style in the abstract.
Response: Thank you for your valuable suggestions, the correction of the language style has been rectified in the abstract to improve the quality of the article.
The introduction gives enough information updated but, in my opinion, we have to distinguished the natural nano-size objects from those manufactured because their role and origin is very different. It seems not so clear in the text. Do authors considered soil microaggregates as nano-size objects with functions similar to those of the MNOs?
Response: Thank you for your valuable comments, actually in the current review the authors have focused upon the manufactured nano-sized objects and its aggregation in the environment especially in the rhizosphere e.g., Many ecosystemic functions in agrosystems are provided by soil microbes, just like the ecosystem of soil the completion of cyclic activity of the vital nutrients like C, N, S and phosphorus is carried out by soil microorganisms. Soil microorganisms affect Carbon nanotubes (CNT), nanoparticles (NPs) and a nanopesticide basically called manufactured nano-objects that are added to the environment intentionally or reaches to the soil in the form of contaminants of nanomaterial. It's critical to assess the influence of MNOs on important plant-microbe symbiosis including mycorrhizas, which are critical for the health, function and sustainability of both natural and agricultural ecosyststems. Toxic compounds are released into rural and urban ecosystems as a result of anthropogenic contamination from industrial processes, agricultural practices, and consumer products.
In several lines, try to avoid cutting the word at the end of the sentence in such way that makes it easy to read, if possible. For instance, lines 53-54, “devel-opment” maybe “deve-lopment”; and others like in line 74-75 “nan-ofibers”.
Response: Thank you for your valuable comment, actually the draft was prepared on susy which automatically bifurcate the words which do not complete at the end of the line so there it shows a hypen and divides the word.
In line 107, please write correctly “Ca+2”.
Response: Thank you for your valuable comment, the suggested correction has been rectified and amended, thanks,
Line 157, please use the citation instead of this sentence: “according to the authors of the reference [24]”
Response: Thank you for your valuable comment, as per the policy of the journal it is suggested in the authors guide lines that reference must be started in such that the authors of the reference etc., so as per guidelines it was done, thanks
The position of tables and figures would be arranged to avoid have them cut between sheets. Congrats, the figures are so nice and interesting.
Response: Thank you for your valuable comment, the suggestion has been rectified in the manuscript and practiced wherever it was appropriate. Thanks.
In general, I think that the article can be published and in my opinion, it is a review of great interest.
Response: Thank you for your encouraging remarks, it has uplifts our moral,
Author Response File: 
Author Response.docx
Reviewer 2 Report
The goal of the review is description of impact of manufactured nano-sized objects on soil biota and microbial community. In this manuscript, authors provide information about effect of oxide metals in general. I have some comments.
Line 35 Abbreviation (MNO) should be defined on their first appearance in the text.
Line 52 The aim of first sentence is not clear.
Line 74 The decryption (NPs) is given twice.
Line 73-76 There are two sentences?
Line 101 Abbreviation (NOM) is not needed. It is given latter (line 103).
Line 146 section 3.3 goes after section 2.
Line 252-253 arbuscularmycorrhizal – what is it?
Line 288-292 Very long sentence. Perhaps, the authors forgot one dot.
Line 306, 383, 398 Bradyrhizobiumjaponicum, Shewanellaaneidensis Shewanellaoneidensis- space?
Line 319 caledoniumto – space?
Figure 3 ROS, SOD, POD etc. what is it?
Line 345 to cell or animal – may be to cell of animals?
Line 358 clorid e coverd – epactal space
Line 382 some word from capital letter
Line 383, 398 Shewanellaaneidensis Shewanellaoneidensis - different spelling
Figure 4 constrcted wetland – mistake
Line 487 PH
Line 538 Gammaproteo bacteria – together
The reference list is large, the authors should check it for compliance in the text.
Line 717: TiO2 can potentially be employed in agriculture [158].
In real, the reference 158 is about silver: 158. Kim, B., Park, C.-S., Murayama, M., Hochella Jr, M.F., 2010. Discovery and characterization of silver sulfide nanoparticles in final sewage sludge products. Environmental science & technology 44, 7509-7514.
Author Response
Point by Point Response to Reviewer-II Comments and Suggestions for Authors
The goal of the review is description of impact of manufactured nano-sized objects on soil biota and microbial community. In this manuscript, authors provide information about effect of oxide metals in general. I have some comments.
Response: Thank you for your encouraging remarks. The corrections has been rectified and amended in tarck changes for consideration please.
Line 35 Abbreviation (MNO) should be defined on their first appearance in the text.
Response: Thank you for your valuable comment, the correction has been made and definition has been written at its first appearance. Thanks,
Line 52 The aim of first sentence is not clear.
Response: Thank you for your valuable comment, the correction has been made and the sentence has been re written in order to make it clear for the readers and to improve the quality of the aticle. According to some scientists, nanoparticles and nanostructured materials may have been formed during the Big Bang and brought to Earth via meteorites. The term "nanotechnology" gained widespread attention in the 1990s due to advances in imaging technologies that enabled practical applications in various industries.
Line 74 The decryption (NPs) is given twice.
Response: Thank you for your valuable comment, the correction has been made. The similar words has been deleted.
Line 73-76 There are two sentences?
Response: Thank you for your valuable comment, this is a composite sentence from line 73 to 76. Thanks,
Line 101 Abbreviation (NOM) is not needed. It is given latter (line 103).
Response: Thank you for your valuable comment, the correction has been made and rectified in track changes as per the light of suggestions.
Line 146 section 3.3 goes after section 2.
Response: Thank you for your valuable comment, the correction has been made as per the light of comments, All the sequence of the sections and sub section has been gone through and rectified, thanks.
Line 252-253 arbuscularmycorrhizal – what is it?
Response: Arbuscular mycorrhiza (AM) is the most common symbiotic association of plants with microbes. AM fungi occur in the majority of natural habitats and they provide a range of important ecological services, in particular by improving plant nutrition, stress resistance and tolerance, soil structure and fertility.
Line 288-292 Very long sentence. Perhaps, the authors forgot one dot.
Response: Thank you for your valuable comment, the correction has been made as per the light of comments, thanks.
Line 306, 383, 398 Bradyrhizobiumjaponicum, Shewanellaaneidensis Shewanellaoneidensis- space?
Response: Thank you for your valuable comment, Bradyrhizobium japonicum (Bradyrhizobium japonicum is found in the root region of the soybean where it forms nodules and fixes nitrogen into a useable form for the soybean plant); Shewanella oneidensis (Shewanella oneidensis is a Gram-negative anaerobic bacteria which is predominantly found in deep sea anaerobic habitats; but can also reside in soil and sedentary habitats. S. oneidensis' ability to reduce and absorb heavy metals makes it a candidate for use in wastewater treatment. DSFO+ could possibly allow the bacteria to electrically communicate with an electrode and generate electricity in a wastewater application. The correction has been made and rectified.
Line 319 caledoniumto – space?
Response: Thank you for your valuable comment, the correction has been made and rectified.
Figure 3 ROS, SOD, POD etc. what is it?
Response: Thank you for your valuable comment, ROS stands for reactive oxygen species, SOD stands for superoxide dismutase and POD stands for peroxidases. These are antioxidants enzymes superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) apparently contributed to ROS accumulation in pod wall tissues.
Line 345 to cell or animal – may be to cell of animals?
Response: Thank you for your valuable comment, cells of animals. Thanks
Line 358 clorid e coverd – epactal space
Response: Thank you for your valuable comment, the correction has been made and rectified.
Line 382 some word from capital letter
Response: Thank you for your valuable comment, the correction has been made and rectified.
Line 383, 398 Shewanellaaneidensis Shewanellaoneidensis - different spelling
Response: Thank you for your valuable comment, the correct spellings are Shewanella oneidensis. The correction has been made and rectified,
Figure 4 constrcted wetland – mistake
Response: Thank you for your valuable comment, the correction has been made and rectified.
Line 487 PH
Response: Thank you for your valuable comment, the correction pH has been made and rectified.
Line 538 Gammaproteo bacteria – together
Response: Thank you for your valuable comment, it is written as Gammaproteobacteria; there is no space in it, correction has been made, thanks.
The reference list is large, the authors should check it for compliance in the text.
Response: Thank you for your valuable comment, the citations has been cross checked with the reference list and rectified, thanks.
Line 717: TiO2 can potentially be employed in agriculture [158].
Response: Thank you for your valuable comment, the correction TiO2 has been made and rectified.
In real, the reference 158 is about silver: 158. Kim, B., Park, C.-S., Murayama, M., Hochella Jr, M.F., 2010. Discovery and characterization of silver sulfide nanoparticles in final sewage sludge products. Environmental science & technology 44, 7509-7514.
Response: Thank you for your valuable comment, yes exactly this reference 158 is about silver sulfide nano particles but this particular sentence has been extracted from this article discussion section just to strengthen the article.
Author Response File: Author Response.docx
Reviewer 3 Report
Please add also what is happening with the addition of bulk agrochemicals and compare.
Write the scientific names in italics. For references, please read the instructions for authors.
Author Response
Point by Point Response to Reviewer-III Comments and Suggestions for Authors
Please add also what is happening with the addition of bulk agrochemicals and compare.
Response: Thank you very much for your valuable comment, the suggested lines has been added in the main manuscript and compared its role in the rhizosphere and overall in the environment.
Write the scientific names in italics. For references, please read the instructions for authors.
Response: Thank you very much for your valuable comment, the whole manuscript has been read carefully and all the scientific names has been italicized.
Author Response File: Author Response.docx
