The Combination of Decellularized Cartilage and Amniotic Membrane Matrix Enhances the Production of Extracellular Matrix Elements in Human Chondrocytes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Human Cartilage Collection and Preparation of Decellularized Articular Cartilage Matrix (dACM)
2.2. Amniotic Membrane Collection and Preparation of Decellularized Amniotic Membrane Matrix (dAMM)
2.3. Histological and Immunohistochemical Analyses
2.4. dACM and dAMM Microstructure Analysis
2.5. FT-IR Spectroscopy
2.6. Chondrocytes Isolation and Cuture
2.7. In Vitro Cell Culture Assays
2.8. Chondrogenic Gene Expression
2.9. Statistical Analysis
3. Results
3.1. Particle Sizes and Assays to Evaluate the Decellularized Process
3.2. Preservation of the Native Structure before and after Decellularized Process
3.3. Proliferative Effect of Individually Soluble Extracts from dACM and dAMM on Human
3.4. Evaluation of the Expression Level of Chondrogenesis-Related Markers
3.5. Analysis the Combination of dACM and dAMM on Cell Proliferation and Chondrogenic Gene Expression
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Rojas-Murillo, A.; Lara-Arias, J.; Leija-Gutiérrez, H.; Franco-Márquez, R.; Moncada-Saucedo, N.K.; Guzmán-López, A.; Vilchez-Cavazos, F.; Garza-Treviño, E.N.; Simental-Mendía, M. The Combination of Decellularized Cartilage and Amniotic Membrane Matrix Enhances the Production of Extracellular Matrix Elements in Human Chondrocytes. Coatings 2024, 14, 1083. https://doi.org/10.3390/coatings14091083
Rojas-Murillo A, Lara-Arias J, Leija-Gutiérrez H, Franco-Márquez R, Moncada-Saucedo NK, Guzmán-López A, Vilchez-Cavazos F, Garza-Treviño EN, Simental-Mendía M. The Combination of Decellularized Cartilage and Amniotic Membrane Matrix Enhances the Production of Extracellular Matrix Elements in Human Chondrocytes. Coatings. 2024; 14(9):1083. https://doi.org/10.3390/coatings14091083
Chicago/Turabian StyleRojas-Murillo, Antonio, Jorge Lara-Arias, Héctor Leija-Gutiérrez, Rodolfo Franco-Márquez, Nidia Karina Moncada-Saucedo, Abel Guzmán-López, Félix Vilchez-Cavazos, Elsa Nancy Garza-Treviño, and Mario Simental-Mendía. 2024. "The Combination of Decellularized Cartilage and Amniotic Membrane Matrix Enhances the Production of Extracellular Matrix Elements in Human Chondrocytes" Coatings 14, no. 9: 1083. https://doi.org/10.3390/coatings14091083