Identification of Differential N-Glycan Compositions in the Serum and Tissue of Colon Cancer Patients by Mass Spectrometry
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Chemical and Reagents
2.2. Patients and Controls
2.3. Samples
2.3.1. Serum
2.3.2. Tissue
2.4. Protein Extraction from Tissue Samples
2.5. N-Glycan Isolation and Derivatization
2.6. Determination of N-Glycan Profile from Blood Samples
2.7. Determination of N-Glycan Profile from Tissue Samples
2.8. Mass Spectrometry Acquisitions and Analysis
2.9. MALDI-TOF/MS
2.10. ESI-LC-MS/MS
3. Results
3.1. Patients with Colon Cancer Present Differential N-Glycans in Their Blood Serum in Relation to Normal Individuals
3.2. N-Glycan Profiles in CRC Tissue Are Heterogeneous, However, Some Glycan Compositions Are Consistently Altered in Patients
3.3. Correlation between Relative Levels of N-Glycans in Serum and Tissue of CRC Patients
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Patient | Genre | Age (years) | Location | Stage | CEA (ng/mL) | Tumor Diameter (cm) | Mutational Nras/Kras/Braf Status |
---|---|---|---|---|---|---|---|
1 | M | 37 | TRANSVERSE | II | 1.48 | 3.70 | Kras mutated |
2 | F | 36 | SIGMOID | II | 1.65 | 2.80 | No |
3 | M | 65 | CECUM | II | 1.60 | 4.30 | Kras mutated |
4 | M | 55 | DESCENDING | III | 5.30 | 2.60 | No |
5 | M | 60 | SIGMOID | II | 7.70 | 4.80 | No |
6 | F | 56 | SIGMOID | III | 2.10 | 3.70 | No |
7 | M | 67 | ASCENDING | III | 2.30 | 6.00 | Braf mutated |
8 | F | 64 | CECUM | III | 11.25 | 4.10 | No |
9 | F | 63 | CECUM | II | 6.51 | 6.20 | No |
10 | F | 54 | SIGMOID | III | 3.30 | 3.50 | Braf mutated |
11 | F | 74 | SIGMOID | III | 6.75 | 1.20 | No |
12 | M | 64 | CECUM | III | 5.20 | 7.20 | Kras mutated |
13 | F | 75 | SIGMOID | II | 1.35 | 4.60 | Kras mutated |
Nr. | Proposed Structure, Theoretical Mass, and Composition | Mass Used for Quantitation | Adduct | Heavy Reference | Structure Confirmed by MS/MS Data a | |
---|---|---|---|---|---|---|
LC-MS/MS | MALDI | |||||
6 | HexNac2Hex5 | 1579.7826 | [M + Na]+ | 52 | 6 | ✓ |
8 | HexNAc3Hex4 | 1620.8090 | [M + Na]+ | 19 | - | * |
10 | HexNAc2Hex6 | 903.4358 | [M + 2Na]2+ | 52 | 6 | ✓✓ |
14 | HexNAc4Hex4 | 1865.9355 | [M + Na]+ | 19 | 19 | ✓✓ |
17 | HexNAc2Hex7 | 1005.4857 * | [M + 2Na]2+ | 52 | 6 | ✓✓ |
22 | HexNAc5Hex4 | 1067.0255 | [M + 2Na]2+ | 19 | - | * |
23 | HexNAc6Hex3 | 1087.5388 * | [M + 2Na]2+ | 13 | - | ✓ |
25 | HexNAc2Hex8 | 1107.5356 | [M + 2Na]2+ | 52 | 6 | ✓✓ |
26 | HexNAc4Hex4NeuAc1 | 1125.0492 | [M + 2Na]2+ | 26 | 35 | ✓✓ |
28 | HexNAc5Hex4Fuc1 | 1154.0701 | [M + 2Na]2+ | 19 | 19 | ✓ |
31 | HexNAc3Hex5Fuc1NeuAc1 | 1191.5805 | [M + 2Na]2+ | 27 | - | ✓✓ |
33 | HexNAc2Hex9 | 1209.5855 | [M + 2Na]2+ | 52 | 6 | ✓✓ |
36 | HexNAc5Hex4NeuAc1 | 1247.6123 | [M + 2Na]2+ | 52 | - | ✓✓ |
40 | HexNAc3Hex6Fuc1NeuAc1 | 1293.6304 * | [M + 2Na]2+ | 27 | - | ✓✓ |
41 | HexNAc7Hex3Fuc1 | 1297.1465 | [M + 2Na]2+ | 75 | - | * |
42 | HexNAc2Hex10 | 1311.6353 | [M + 2Na]2+ | 52 | - | ✓ |
43 | HexNAc7Hex4 | 1312.1518 | [M + 2Na]2+ | 72 | - | ✓ |
44 | HexNAc4Hex5Fuc1NeuAc1 | 1314.1437 | [M + 2Na]2+ | 44 | 58 | ✓✓ |
49 | HexNAc5Hex5NeuAc1 | 907.4379 | [M + 3Na]3+ | 35 | ✓ | |
51 | HexNAc6Hex6 | 936.7887 | [M + 3Na]3+ | 52 | 35 | ✓ |
52 | HexNAc4Hex5NeuAc2 | 1407.6859 | [M + 2Na]2+ | 52 | 52 | ✓✓ |
55 | HexNAc5Hex6NeuAc1 | 975.4711 | [M + 3Na]3+ | 72 | 35 | ✓✓ |
56 | HexNAc6Hex5Fuc2 | 984.8149 | [M + 3Na]3+ | 27 | - | ✓ |
57 | HexNAc6Hex6Fuc1 | 994.8184 | [M + 3Na]3+ | 27 | - | ✓ |
58 | HexNAc4Hex5Fuc1NeuAc2 | 1004.1501 | [M + 3Na]3+ | 58 | 58 | ✓✓ |
61 | HexNAc5Hex6Fuc1NeuAc1 | 1033.5009 * | [M + 3Na]3+ | 27 | - | ✓ |
64 | HexNAc5Hex5Fuc1NeuAc2 | 1617.2937 | [M + 2Na]2+ | 27 | 64 | ✓ |
66 | HexNAc6Hex6Fuc1NeuAc1 | 1115.2096 | [M + 3Na]3+ | 27 | - | ✓ |
67 | HexNAc6Hex7Fuc2 | 1669.8275 * | [M + 2Na]2+ | 27 | - | ✓ |
79 | HexNAc6Hex7NeuAc3 | 1030.2440 | [M + 4Na]4+ | 80 | 52 | ✓ |
85 | HexNAc7Hex8NeuAc4 | 1232.8439 * | [M + 4Na]4+ | 80 | - | * |
86 | HexNAc7Hex8Fuc1NeuAc4 | 1276.3663 * | [M + 4Na]4+ | 80 | - | ✓ |
87 | HexNAc4Hex3Fuc2 | 1016.5017 | [M + 2Na]2+ | 13 | - | ✓✓ |
Nr | N-Glycan Composition | Median Controls (n = 11) | Median Cancer Patients (n = 13) | Alteration in Cancer Patients | Z | Wilcoxon, 2-Sample Test, Normal Approximation, Prob>|Z| |
---|---|---|---|---|---|---|
MALDI-TOF/MS data | ||||||
49 | HexNAc5Hex5NeuAc1 | 0 | 0.022 | ↑ | −3.09 | 0.0020 |
LC-MS/MS data | ||||||
8 | HexNAc3Hex4 | 0.140 | 0.012 | ↓ | 3.13 | 0.0018 |
14 | HexNAc4Hex4 | 0.685 | 0.023 | ↓ | 3.03 | 0.001 |
17 | HexNAc2Hex7 | 0.000 | 0.026 | ↑ | −3.53 | 0.0004 |
22 | HexNAc5Hex4 | 0.882 | 0.020 | ↓ | 3.77 | 0.0002 |
23 | HexNAc6Hex3 | 0.466 | 0.005 | ↓ | 2.72 | 0.0065 |
25 | HexNAc2Hex8 | 0.149 | 0.051 | ↓ | 2.72 | 0.0065 |
26 | HexNAc4Hex4NeuAc1 | 1.619 | 0.160 | ↓ | 3.30 | 0.001 |
31 | HeXNAc3Hex5Fuc1NeuAc1 | 4.228 | 0.021 | ↓ | 3.07 | 0.0021 |
33 | HexNAc2Hex9 | 0.334 | 0.072 | ↓ | 3.01 | 0.0026 |
36 | HexNAc5Hex4NeuAc1 | 0.838 | 0.035 | ↓ | 3.13 | 0.0018 |
40 | HexNAc3Hex6Fuc1NeuAc1 | 0.413 | 0.000 | ↓ | 2.97 | 0.003 |
41 | HexNAc7Hex3Fuc1 | 1.533 | 0.092 | ↓ | 3.25 | 0.0012 |
42 | HexNAc2Hex10 | 0.147 | 0.008 | ↓ | 3.42 | 0.0006 |
43 | HexNAc7Hex4 | 2.705 | 0.025 | ↓ | 3.36 | 0.0008 |
44 | HexNAc4Hex5Fuc1NeuAc1 | 1.659 | 0.772 | ↓ | 3.01 | 0.0026 |
51 | HexNAc6Hex6 | 9.144 | 0.842 | ↓ | 3.42 | 0.0006 |
55 | HexNAc5Hex6NeuAc1 | 0.650 | 0.123 | ↓ | 2.66 | 0.0077 |
56 | HexNAc6Hex5Fuc2 | 0.221 | 0.004 | ↓ | 3.02 | 0.0026 |
57 | HexNAc6Hex6Fuc1 | 0.439 | 0.030 | ↓ | 2.66 | 0.0077 |
58 | HexNAc4Hex5Fuc1NeuAc2 | 0.315 | 0.987 | ↑ | −3.36 | 0.0008 |
61 | HexNAc5Hex6Fuc1NeuAc1 | 0.414 | 0.069 | ↓ | 2.84 | 0.0045 |
66 | HexNAc6Hex6Fuc1NeuAc1 | 0.633 | 0.050 | ↓ | 3.07 | 0.0021 |
79 | HexNAc6Hex7NeuAc3 | 0.001 | 0.226 | ↑ | −3.19 | 0.0014 |
85 | HexNAc7Hex8NeuAc4 | 1.188 | 0.000 | ↓ | 3.44 | 0.0006 |
86 | HexNAc7Hex8Fuc1NeuAc4 | 0.920 | 0.019 | ↓ | 2.95 | 0.0031 |
87 | HexNAc4Hex3Fuc2 | 0.379 | 0.006 | ↓ | 2.95 | 0.0031 |
Patient Nr. | |||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Nr. | N-Glycan Composition | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | Median | n/Total * | Binomial Probability |
MALDI-TOF/MS data (area ratio) | |||||||||||||||||
6 | HexNac2Hex5 | 29.41 | 62.5 | - | 6.88 | 15.33 | - | 7.48 | 20.8 | 0.2 | 3.09 | 14.63 | - | 27.09 | 14.98 | 9/10 | 0.0010 |
10 | HexNac2Hex6 | 42.63 | 7.25 | - | 8.71 | - | - | 21.94 | 4 | 0.06 | - | 7.48 | - | 23.4 | 8.10 | 7/8 | 0.0020 |
LC-MS/MS data (area ratio) | |||||||||||||||||
17 | HexNAc2Hex7 | - | - | - | 0.02 | 0.03 | 0.00 | 0.03 | 0.02 | 0.01 | 0.11 | 0.01 | - | - | 0.02 | 8/8 | 0.0000 |
22 | HexNAc5Hex4 | 167.48 | 53.17 | 31.47 | 38.23 | 84.42 | - | 7.42 | 110.19 | 1.44 | 4.61 | 6.39 | - | 136.41 | 38.23 | 11/11 | 0.0000 |
26 | HexNAc4Hex4NeuAc1 | 40.70 | 31.47 | 11.41 | 10.34 | 5.26 | - | 1.64 | 24.58 | - | - | 3.43 | - | 26.69 | 11.41 | 9/9 | 0.0000 |
28 | HexNAc5Hex4Fuc1 | 51.49 | 17.16 | 46.90 | - | 4.20 | - | - | 43.48 | 0.79 | 1.84 | 5.27 | - | 19.55 | 17.16 | 8/9 | 0.0020 |
33 | HexNAc2Hex9 | 92.23 | 116.52 | 86.94 | 65.26 | 5.54 | - | 4.45 | 32.67 | 0.52 | - | 16.76 | - | 123.43 | 48.96 | 9/10 | 0.0010 |
36 | HexNAc5Hex4NeuAc1 | 140.70 | 113.15 | 59.98 | 142.75 | 17.23 | 0.06 | 1.98 | 35.80 | 0.78 | 1.70 | 56.29 | - | 587.41 | 46.05 | 10/12 | 0.0032 |
41 | HexNAc7Hex3Fuc1 | 20.10 | 26.13 | 21.01 | 10.27 | 27.09 | - | - | 20.10 | - | - | 4.52 | - | 59.21 | 20.55 | 8/8 | 0.0000 |
43 | HexNAc7Hex4 | 26.12 | 10.94 | 25.56 | 6.74 | - | 0.01 | 1.34 | 7.67 | 0.40 | 1.06 | 1.91 | - | 14.29 | 6.74 | 9/11 | 0.0059 |
57 | HexNAc6Hex6Fuc1 | - | 12.13 | 43.48 | 12.63 | 6.20 | 2.52 | 1.76 | 7.29 | 0.97 | 2.32 | 4.52 | 1.76 | 26.01 | 5.36 | 11/12 | 0.0002 |
64 | HexNAc5Hex5Fuc1NeuAc2 | 7.49 | 24.86 | 6.61 | 14.67 | - | 0.62 | - | - | 0.45 | 2.54 | 11.36 | - | 19.28 | 7.49 | 7/9 | 0.0195 |
67 | HexNAc6Hex7Fuc2 | 9.62 | 7.77 | 5.74 | 23.82 | 16.50 | - | - | 13.98 | - | - | - | - | 5.50 | 9.62 | 7/7 | 0.0000 |
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Coura, M.d.M.A.; Barbosa, E.A.; Brand, G.D.; Bloch, C., Jr.; de Sousa, J.B. Identification of Differential N-Glycan Compositions in the Serum and Tissue of Colon Cancer Patients by Mass Spectrometry. Biology 2021, 10, 343. https://doi.org/10.3390/biology10040343
Coura MdMA, Barbosa EA, Brand GD, Bloch C Jr., de Sousa JB. Identification of Differential N-Glycan Compositions in the Serum and Tissue of Colon Cancer Patients by Mass Spectrometry. Biology. 2021; 10(4):343. https://doi.org/10.3390/biology10040343
Chicago/Turabian StyleCoura, Marcelo de M.A., Eder A. Barbosa, Guilherme D. Brand, Carlos Bloch, Jr., and Joao B. de Sousa. 2021. "Identification of Differential N-Glycan Compositions in the Serum and Tissue of Colon Cancer Patients by Mass Spectrometry" Biology 10, no. 4: 343. https://doi.org/10.3390/biology10040343
APA StyleCoura, M. d. M. A., Barbosa, E. A., Brand, G. D., Bloch, C., Jr., & de Sousa, J. B. (2021). Identification of Differential N-Glycan Compositions in the Serum and Tissue of Colon Cancer Patients by Mass Spectrometry. Biology, 10(4), 343. https://doi.org/10.3390/biology10040343