Biology

16 pages, 2976 KiB

Open AccessArticle

Integrating Computational Analysis of In Vivo Investigation of Modulatory Effect of Fagonia cretica Plant Extract on Letrozole-Induced Polycystic Ovary Syndrome in Female Rats

by Ayesha Qasim, Hiram Calvo, Jesús Jaime Moreno Escobar and Zia-ud-din Akhtar

Biology 2025, 14(7), 903; https://doi.org/10.3390/biology14070903 - 21 Jul 2025

Abstract

Fagonia cretica, a medicinal herb from the Zygophyllaceae family, is traditionally utilized to treat various conditions such as hepatitis, gynecological disorders, tumors, urinary tract issues, and diabetes. The present study aimed to evaluate the therapeutic potential of Fagonia cretica in treating polycystic [...] Read more.

Fagonia cretica, a medicinal herb from the Zygophyllaceae family, is traditionally utilized to treat various conditions such as hepatitis, gynecological disorders, tumors, urinary tract issues, and diabetes. The present study aimed to evaluate the therapeutic potential of Fagonia cretica in treating polycystic ovarian syndrome (PCOS) induced in female rats. PCOS, a complex hormonal disorder, was experimentally induced by administering Letrozole (1 mg/kg) in combination with a high-fat diet for 21 days. The affected rats were then treated with hydro-alcoholic extracts of Fagonia cretica at doses of 100 mg/kg, 200 mg/kg, and 300 mg/kg for 20 days. Key biochemical parameters—including serum testosterone, insulin, fasting blood glucose, insulin resistance (HOMA-IR), cholesterol, triglycerides, and lipoprotein levels—were measured. Ultrasound imaging and histopathological analysis of ovarian tissues were also performed. The data were analyzed using computer-based statistical tools, including one-way ANOVA, Cohen’s d effect size, and Tukey’s HSD test, with graphical representations generated using Python 3.10 on the Kaggle platform. Results demonstrated a significant reduction in serum testosterone, insulin, cholesterol, and triglyceride levels (p < 0.05) in treated groups, along with improved ovarian morphology. These findings support the therapeutic potential of Fagonia cretica as a natural treatment for PCOS. Full article

20 pages, 2290 KiB

Open AccessArticle

Use of Bacillus pretiosus and Pseudomonas agronomica for the Synthesis of a Valorized Water Waste Treatment Plant Waste as a Biofertilizer Intended for Quercus pyrenaica L. Fertigation

by Diana Penalba-Iglesias, Marina Robas-Mora, Daniel González-Reguero, Vanesa M. Fernández-Pastrana, Agustín Probanza and Pedro A. Jiménez-Gómez

Biology 2025, 14(7), 902; https://doi.org/10.3390/biology14070902 - 21 Jul 2025

Abstract

The loss of hectares of forest areas has become a global issue that has worsened over recent years due to unsustainable human activities. In a context of limited availability of productive land, it is urgent to adopt efficient strategies to recover the affected [...] Read more.

The loss of hectares of forest areas has become a global issue that has worsened over recent years due to unsustainable human activities. In a context of limited availability of productive land, it is urgent to adopt efficient strategies to recover the affected natural areas. Actions based on a circular economy, such as the use of organic chemical matrices recovered from water waste treatment plant waste, have proven to be effective. In this regard, the addition of plant growth-promoting bacteria (PGPB), such as Bacillus pretiosus and Pseudomonas agronomica, can contribute to the chemical treatment, favoring the recovery of soils, accelerating the recovery of vegetation cover, and inducing an increase in biodiversity. In this research, the effect of bio-fertigation under controlled laboratory conditions in Quercus pyrenaica is evaluated. After a thirty-six-week trial, the biometric and nutritional parameters of the plants were harvested and measured, and the diversity and composition of the metagenomes of their rhizospheres were evaluated. As well, the cenoantibiogram and the metabolic diversity were measured. The results showed that the use of these biofertilizers increased the variables related to plant production, quality of plant composition as an indirect means of their resilience, as well as an increase in rhizospheric microbial diversity and a reduction in their MIC resistance to the most widely used antibiotics. For all these reasons, the use of the biofertilizer result of the combination of WWTP waste, Bacillus pretiosus, and Pseudomonas agronomica is postulated as an environmentally friendly strategy that can contribute to the recovery of potential oak forest areas. Full article

(This article belongs to the Collection Plant Growth-Promoting Bacteria: Mechanisms and Applications)

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24 pages, 2487 KiB

Open AccessArticle

Community Structure and Influencing Factors of Macro-Benthos in Bottom-Seeded Marine Pastures: A Case Study of Caofeidian, China

by Xiangping Xue, Long Yun, Zhaohui Sun, Jiangwei Zan, Xinjing Xu, Xia Liu, Song Gao, Guangyu Wang, Mingshuai Liu and Fei Si

Biology 2025, 14(7), 901; https://doi.org/10.3390/biology14070901 - 21 Jul 2025

Abstract

To accurately assess the water quality, ecosystem status, distribution of large benthic organisms, and ecological restoration under human intervention, an analysis of benthic organisms on Caofeidian in September and November 2023 and January and May of the following year was conducted in this [...] Read more.

To accurately assess the water quality, ecosystem status, distribution of large benthic organisms, and ecological restoration under human intervention, an analysis of benthic organisms on Caofeidian in September and November 2023 and January and May of the following year was conducted in this work. By performing CCA (canonical correspondence analysis) and cluster and correlation coefficient (Pearson) analyses, the temporal variation characteristics of benthic abundance, dominant species, community structure and biodiversity were analyzed. A total of 79 species of macro-benthic animals were found in four months, including 32 species of polychaetes, cnidarians, 1 species of Nemertean, 19 species of crustaceans, and 24 species of molluscs. The use of conventional grab-type mud collectors revealed that the Musculus senhousei dominated the survey (Y > 0.02). While only a small number of Ruditapes philippinarum were collected from bottom-dwelling species, a certain number of bottom-dwelling species (Ruditapes philippinarum and Scapharca subcrenata) were also collected during the trawl survey. Additionally, a significant population of Rapana venosa was found in the area. It is speculated that the dual effects of predation and competition are likely the primary reasons for the relatively low abundance of bottom-dwelling species. The density and biomass of macro-benthos were consistent over time, which was the highest in May, the second highest in January, and the lowest in September and November. The main environmental factors affecting the large benthic communities in the surveyed sea areas were pH, DO, NO₂⁻-N, T, SAL and PO₄³⁻-P. Combined with historical data, it was found that although the environmental condition in the Caofeidian sea area has improved, the Musculus senhousei has been dominant. In addition, the abundance of other species is much less than that of the Musculus senhousei, and the diversity of the benthic community is still reduced. Our work provides valuable data support for the management and improvement of bottom Marine pasture and promotes the transformation of Marine resources from resource plunder to a sustainable resource. Full article

(This article belongs to the Special Issue Global Fisheries Resources, Fisheries, and Carbon-Sink Fisheries)

18 pages, 1336 KiB

Open AccessArticle

Modeling Unveils How Kleptoplastidy Affects Mixotrophy Boosting Algal Blooms

by Irena V. Telesh, Gregory J. Rodin, Hendrik Schubert and Sergei O. Skarlato

Biology 2025, 14(7), 900; https://doi.org/10.3390/biology14070900 - 21 Jul 2025

Abstract

Kleptoplastidy is a nutrition mode in which cells of protists and some multicellular organisms acquire, maintain, and exploit chloroplasts of prey algae cells as photosynthesis reactors. It is an important aspect of the mixotrophic feeding strategy, which plays a role in the formation [...] Read more.

Kleptoplastidy is a nutrition mode in which cells of protists and some multicellular organisms acquire, maintain, and exploit chloroplasts of prey algae cells as photosynthesis reactors. It is an important aspect of the mixotrophic feeding strategy, which plays a role in the formation of harmful algae blooms (HABs). We developed a new mathematical model, in which kleptoplastidy is regarded as a mechanism of enhancing mixotrophy of protists. The model is constructed using three thought (theoretical) experiments and the concept of biological time. We propose to measure the contribution of kleptoplastidy to mixotrophy using a new ecological indicator: the kleptoplastidy index. This index is a function of two dimensionless variables, one representing the ratio of photosynthetic production of acquired chloroplasts versus native chloroplasts, and the other representing the balance between autotrophic and heterotrophic feeding modes. The index is tested by data for the globally distributed, bloom-forming potentially toxic mixotrophic dinoflagellates Prorocentrum cordatum. The model supports our hypothesis that kleptoplastidy can increase the division rate of algae significantly (by 40%), thus boosting their population growth and promoting blooms. The proposed model can contribute to advancements in ecological modeling aimed at forecasting and management of HABs that deteriorate marine coastal environments worldwide. Full article

(This article belongs to the Section Theoretical Biology and Biomathematics)

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22 pages, 5908 KiB

Open AccessArticle

MaxEnt Modeling of Future Habitat Shifts of Itea yunnanensis in China Under Climate Change Scenarios

by Jinxin Zhang, Xiaoju Li, Suhang Li, Qiong Yang, Yuan Li, Yangzhou Xiang and Bin Yao

Biology 2025, 14(7), 899; https://doi.org/10.3390/biology14070899 - 21 Jul 2025

Abstract

The distribution of Itea yunnanensis, a shrub species in the genus Itea of the family Iteaceae, is primarily concentrated in the Hengduan Mountains region of China, where it faces severe threats from global climate change. However, systematic research on the species’ [...] Read more.

The distribution of Itea yunnanensis, a shrub species in the genus Itea of the family Iteaceae, is primarily concentrated in the Hengduan Mountains region of China, where it faces severe threats from global climate change. However, systematic research on the species’ distribution patterns, climatic response mechanisms, and future suitable habitat dynamics remains insufficient. This study aims to assess the spatiotemporal evolution and driving mechanisms of I. yunnanensis-suitable habitats under current and future climate change scenarios to reveal the migration patterns of its distribution centroid and ecological thresholds, and to enhance the reliability and interpretability of predictions through model optimization. For MaxEnt modeling, we utilized 142 georeferenced occurrence records of I. yunnanensis alongside environmental data under current conditions and three future Shared Socioeconomic Pathways (SSPs: SSP1-2.6, SSP2-4.5, SSP5-8.5). Model parameter optimization (Regularization Multiplier, Feature Combination) was performed using the R (v4.2.1) package ‘ENMeval’. The optimized model (RM = 3.0, FC = QHPT) significantly reduced overfitting risk (ΔAICc = 0) and achieved high prediction accuracy (AUC = 0.968). Under current climate conditions, the total area of potential high-suitability habitats for I. yunnanensis is approximately 94.88 × 10⁴ km², accounting for 9.88% of China’s land area, with core areas located around the Hengduan Mountains. Under future climate change, the suitable habitats show significant divergence, area fluctuation and contraction under the SSP1-2.6 scenario, and continuous expansion under the SSP5-8.5 scenario. Meanwhile, the species’ distribution centroid exhibits an overall trend of northwestward migration. This study not only provides key spatial decision-making support for the in situ and ex situ conservation of I. yunnanensis, but also offers an important methodological reference for the adaptive research on other ecologically vulnerable species facing climate change through its optimized modeling framework. Full article

(This article belongs to the Section Conservation Biology and Biodiversity)

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16 pages, 4761 KiB

Open AccessArticle

Metabolic Alterations in Crassostrea Gigas After Feeding Selenium-Enriched Yeast Based on Transcriptomic Analysis

by Yancheng Zhao, Xiaojing Jiang, Liming Jiang, Yongjie Wang, Cuiju Cui, Xiumei Liu, Zan Li, Weijun Wang and Jianmin Yang

Biology 2025, 14(7), 898; https://doi.org/10.3390/biology14070898 - 21 Jul 2025

Abstract

This study explores the effects of selenium-enriched yeast supplementation on growth-related and immune-related gene expression in C. gigas, aiming to support feed optimization in oyster aquaculture. Selenium, an essential trace element, is vital for growth, immune function, and metabolism in animals. Selenium-enriched [...] Read more.

This study explores the effects of selenium-enriched yeast supplementation on growth-related and immune-related gene expression in C. gigas, aiming to support feed optimization in oyster aquaculture. Selenium, an essential trace element, is vital for growth, immune function, and metabolism in animals. Selenium-enriched yeast, an organic form, offers superior bioavailability, enabling efficient absorption and utilization. C. gigas, a commercially significant marine shellfish, is rich in protein and nutrients, but the effects of selenium on mollusks remain insufficiently explored. In this study, oysters were divided into three groups: a control group without selenium (THNP), a 2 ppm selenium group (THMP), and a 4 ppm selenium group (THHP). Transcriptome sequencing yielded 388,679,026 clean reads. GO and KEGG enrichment analyses identified key metabolic signaling pathways, and a PPI analysis was performed on the translation products of DEGs involved in the KEGG pathways. qRT-PCR validated the expression of principal DEGs. The combined results of enrichment and PPI analyses highlighted pathways such as glutathione metabolism and collagen signaling. Additionally, three hub genes—FASN, HRAS, and ABCG5—were identified as central to the selenium response. These findings enhance the understanding of selenium’s molecular impact on oysters and support its application in aquaculture. Full article

(This article belongs to the Special Issue Aquatic Economic Animal Breeding and Healthy Farming)

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18 pages, 6196 KiB

Open AccessArticle

Long-Term Monitoring Reveals Changes in the Small Mammal Community Composition and Co-Occurrence Patterns in the Diannan Area of Yunnan, China

by Jinyu Yang, Ting Jia, Wanlong Zhu and Xiaomi Yang

Biology 2025, 14(7), 897; https://doi.org/10.3390/biology14070897 - 21 Jul 2025

Abstract

Long-term monitoring of small mammal communities provides critical insights into biodiversity conservation by detecting ecosystem degradation and quantifying anthropogenic impacts. Using 13 years (2005–2017) of standardized live-trapping data from the Diannan area, China, we analyzed 22 small mammal species to assess population dynamics [...] Read more.

Long-term monitoring of small mammal communities provides critical insights into biodiversity conservation by detecting ecosystem degradation and quantifying anthropogenic impacts. Using 13 years (2005–2017) of standardized live-trapping data from the Diannan area, China, we analyzed 22 small mammal species to assess population dynamics and community restructuring through co-occurrence network analysis, species composition trends, and multi-index diversity evaluation (Shannon–Wiener, Margalef, Simpson, and Pielou). The research results showed that, except for the two dominant species, Eothenomys miletus and Apodemus chevrieri, whose populations showed an increasing trend during the survey period, the populations of all other species showed a decreasing trend, and some species even faced local extinction. The species diversity index significantly decreased, and the complexity of the co-occurrence network structure also appeared at the local level. We observed a decrease in the diversity of small mammals and the interactions between species. Pearson correlation and redundancy analysis (RDA) revealed that temperature, precipitation, and sunshine duration were the primary environmental drivers of the observed temporal variations in small mammal community structure. These results emphasize the necessity of further conservation efforts to protect local ecosystems and mitigate the negative impact of human activities on the environment. Full article

(This article belongs to the Section Conservation Biology and Biodiversity)

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24 pages, 7124 KiB

Open AccessArticle

In Silico Discovery of a Novel Potential Allosteric PI3Kα Inhibitor Incorporating 3-(2-Chloro-5-fluorophenyl)isoindolin-1-one to Target Head and Neck Squamous Cell Carcinoma

by Wenqing Jia and Xianchao Cheng

Biology 2025, 14(7), 896; https://doi.org/10.3390/biology14070896 - 21 Jul 2025

Abstract

Phosphatidylinositol 3-kinase alpha (PI3Kα) is frequently mutated in head and neck squamous cell carcinoma (HNSCC), leading to the constitutive activation of the PI3K/Akt pathway, which promotes tumor cell proliferation, survival, and metastasis. PI3Kα allosteric inhibitors demonstrate therapeutic potential as both monotherapy and combination [...] Read more.

Phosphatidylinositol 3-kinase alpha (PI3Kα) is frequently mutated in head and neck squamous cell carcinoma (HNSCC), leading to the constitutive activation of the PI3K/Akt pathway, which promotes tumor cell proliferation, survival, and metastasis. PI3Kα allosteric inhibitors demonstrate therapeutic potential as both monotherapy and combination therapy, particularly in patients with PIK3CA mutations or resistance to immunotherapy, through the precise targeting of mutant PI3Kα. Compared to ATP-competitive PI3Kα inhibitors such as Alpelisib, the allosteric inhibitor RLY-2608 exhibits enhanced selectivity for mutant PI3Kα while minimizing the inhibition of wild-type PI3Kα, thereby reducing side effects such as hyperglycemia. To date, no allosteric PI3Kα inhibitors have been approved for clinical use. To develop novel PI3Kα inhibitors with improved safety and efficacy, we employed a scaffold hopping approach to structurally modify RLY-2608 and constructed a compound library. Based on the structural information of the PI3Kα allosteric site, we conducted the systematic virtual screening of 11,550 molecules from databases to identify lead compounds. Through integrated approaches, including molecular docking studies, target validation, druggability evaluation, molecular dynamics simulations, and metabolic pathway and metabolite analyses, we successfully identified a promising novel allosteric PI3Kα inhibitor, H-18 (3-(2-chloro-5-fluorophenyl)isoindolin-1-one). H-18 has not been previously reported as a PI3Kα inhibitor, and provides an excellent foundation for subsequent lead optimization, offering a significant starting point for the development of more potent PI3Kα allosteric inhibitors. Full article

(This article belongs to the Special Issue Protein Kinases: Key Players in Carcinogenesis)

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17 pages, 4255 KiB

Open AccessArticle

Exploring the Global and Regional Factors Influencing the Density of Trachurus japonicus in the South China Sea

by Mingshuai Sun, Yaquan Li, Zuozhi Chen, Youwei Xu, Yutao Yang, Yan Zhang, Yalan Peng and Haoda Zhou

Biology 2025, 14(7), 895; https://doi.org/10.3390/biology14070895 - 21 Jul 2025

Abstract

In this cross-disciplinary investigation, we uncover a suite of previously unexamined factors and their intricate interplay that hold causal relationships with the distribution of Trachurus japonicus in the northern reaches of the South China Sea, thereby extending the existing research paradigms. Leveraging advanced [...] Read more.

In this cross-disciplinary investigation, we uncover a suite of previously unexamined factors and their intricate interplay that hold causal relationships with the distribution of Trachurus japonicus in the northern reaches of the South China Sea, thereby extending the existing research paradigms. Leveraging advanced machine learning algorithms and causal inference, our robust experimental design uncovered nine key global and regional factors affecting the distribution of T. japonicus density. A robust experimental design identified nine key factors significantly influencing this density: mean sea-level pressure (msl-0, msl-4), surface pressure (sp-0, sp-4), Summit ozone concentration (Ozone_sum), F10.7 solar flux index (F10.7_index), nitrate concentration at 20 m depth (N3M20), sonar-detected effective vertical range beneath the surface (Height), and survey month (Month). Crucially, stable causal relationships were identified among Ozone_sum, F10.7_index, Height, and N3M20. Variations in Ozone_sum likely impact surface UV radiation levels, influencing plankton dynamics (a primary food source) and potentially larval/juvenile fish survival. The F10.7_index, reflecting solar activity, may affect geomagnetic fields, potentially influencing the migration and orientation behavior of T. japonicus. N3M20 directly modulates primary productivity by limiting phytoplankton growth, thereby shaping the availability and distribution of prey organisms throughout the food web. Height defines the vertical habitat range acoustically detectable, intrinsically linking directly to the vertical distribution and availability of the fish stock itself. Surface pressures (msl-0/sp-0) and their lagged effects (msl-4/sp-4) significantly influence sea surface temperature profiles, ocean currents, and stratification, all critical determinants of suitable habitats and prey aggregation. The strong influence of Month predominantly reflects seasonal changes in water temperature, reproductive cycles, and associated shifts in nutrient supply and plankton blooms. Rigorous robustness checks (Data Subset and Random Common Cause Refutation) confirmed the reliability and consistency of these causal findings. This elucidation of the distinct biological and physical pathways linking these diverse factors leading to T. japonicus density provides a significantly improved foundation for predicting distribution patterns globally and offers concrete scientific insights for sustainable fishery management strategies. Full article

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16 pages, 3066 KiB

Open AccessArticle

TLR4 Asp299Gly SNP (rs4986790) Protects from Periodontal Inflammatory Destruction by Altering TLR4 Susceptibility to LPS Stimulation

by Franco Cavalla, Claudia C. Biguetti, Ariadne Letra, Renato M. Silva, Alexandre R. Vieira, Franz J. Strauss and Gustavo P. Garlet

Biology 2025, 14(7), 894; https://doi.org/10.3390/biology14070894 - 21 Jul 2025

Abstract

Periodontitis is a multifactorial disease linked to host immune response and genetic predisposition. The TLR4 Asp299Gly single-nucleotide polymorphism (SNP, rs4986790) has been associated with altered responses to bacterial lipopolysaccharide (LPS) and may influence susceptibility to inflammatory diseases. Given the central role of TLR4 [...] Read more.

Periodontitis is a multifactorial disease linked to host immune response and genetic predisposition. The TLR4 Asp299Gly single-nucleotide polymorphism (SNP, rs4986790) has been associated with altered responses to bacterial lipopolysaccharide (LPS) and may influence susceptibility to inflammatory diseases. Given the central role of TLR4 in innate immune recognition of periodontal pathogens, this study investigates the role of rs4986790 in modulating susceptibility to periodontal inflammatory destruction. A total of 1410 individuals from four populations were genotyped, with findings indicating a significant protective effect of the polymorphic allele. Functional assays demonstrated enhanced IL-8 secretion and increased sensitivity to CD14 inhibition in cells expressing the variant receptor. These results suggest that rs4986790 modifies the LPS response via TLR4, potentially offering protection against periodontal breakdown. Full article

(This article belongs to the Section Genetics and Genomics)

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16 pages, 2972 KiB

Open AccessArticle

Protective Effects of N-Acetylcysteine in Alleviating Cocaine-Mediated Microglial Activation and Neuroinflammation

by Uma Maheswari Deshetty, Abiola Oladapo, Yazhini Mohankumar, Elias Horanieh, Shilpa Buch and Palsamy Periyasamy

Biology 2025, 14(7), 893; https://doi.org/10.3390/biology14070893 - 20 Jul 2025

Abstract

Cocaine misuse induces microglial activation and neuroinflammation, contributing to neurodegeneration and behavioral impairments. Prior studies have shown that cocaine induces mitochondrial dysfunction, dysregulated mitophagy, and lysosomal impairment in microglia. Here, we investigated the therapeutic potential of N-acetylcysteine (NAC) in mitigating cocaine-induced microglial activation [...] Read more.

Cocaine misuse induces microglial activation and neuroinflammation, contributing to neurodegeneration and behavioral impairments. Prior studies have shown that cocaine induces mitochondrial dysfunction, dysregulated mitophagy, and lysosomal impairment in microglia. Here, we investigated the therapeutic potential of N-acetylcysteine (NAC) in mitigating cocaine-induced microglial activation and neuroinflammation. Mouse primary microglial cells (MPMs) were pretreated with NAC (5 mM) for 1 h prior to cocaine exposure (10 µM, 24 h) and analyzed for markers of microglial activation, mitophagy, and lysosomal integrity using Western blot, Seahorse assays, lysosomal pH, and membrane potential measurements. In vivo, C57BL/6N mice received NAC (200 mg/kg, i.p.) 1 h before daily cocaine injections (20 mg/kg, i.p.) for 7 days. Behavioral assays (open field, novel object recognition) and brain biomarker analyses (frontal cortex, hippocampus) were performed. Cocaine exposure elevated CD11b, mitophagy markers (PINK1, PARK, and DLP1), and autophagy proteins (Beclin1, and p62), while impairing mitochondrial and lysosomal functions. NAC pretreatment restored mitochondrial and lysosomal function, reduced reactive oxygen species, and normalized protein expression. In vivo, NAC also alleviated cocaine-induced microglial activation and behavioral deficits. These findings highlight NAC as a promising therapeutic agent to counteract cocaine-mediated neuroinflammation and neurotoxicity. Full article

(This article belongs to the Section Biochemistry and Molecular Biology)

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20 pages, 2031 KiB

Open AccessReview

Anti-Inflammatory Pathways Mediating tDCS’s Effects on Neuropathic Pain

by Haipeng Zhang, Xinyan Zheng and Binn Zhang

Biology 2025, 14(7), 892; https://doi.org/10.3390/biology14070892 - 20 Jul 2025

Abstract

Neuropathic pain (NP) is a prevalent clinical condition resulting from diseases or injuries affecting the somatosensory system. Conventional analgesics often exhibit limited efficacy, leading to suboptimal therapeutic outcomes. The pathogenesis of NP is complex and involves multiple mechanisms. The existing evidence suggests that [...] Read more.

Neuropathic pain (NP) is a prevalent clinical condition resulting from diseases or injuries affecting the somatosensory system. Conventional analgesics often exhibit limited efficacy, leading to suboptimal therapeutic outcomes. The pathogenesis of NP is complex and involves multiple mechanisms. The existing evidence suggests that maladaptive neuronal plasticity plays a central role in NP development. Additionally, emerging research highlights the contribution of neuroinflammatory responses mediated by glial cells in the onset of NP and associated sensory hypersensitivity. Among non-invasive neuromodulation techniques, transcranial direct current stimulation (tDCS) has gained prominence as a potential treatment for NP. Numerous studies have demonstrated its analgesic effects; however, the precise regulatory mechanisms remain unclear. The current evidence indicates that tDCS may alleviate NP by enhancing glial–neuronal interactions, which suppress nociceptive signaling pathways and reduce pain sensitivity. The reciprocal modulation between tDCS-mediated anti-inflammatory actions, as evidenced by decreased levels of pro-inflammatory cytokines and increased levels of anti-inflammatory mediators, and its facilitation of adaptive neural plasticity represents a particularly compelling therapeutic axis. This review elucidates inflammatory regulation by tDCS as a fundamental mechanism for NP alleviation, while delineating important unresolved questions regarding these complex interactions. Full article

(This article belongs to the Section Immunology)

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20 pages, 19327 KiB

Open AccessArticle

Genome-Wide Identification of the SRS Gene Family in Cucurbitaceae: Clade Identification and Expression Analysis of CmSRS Genes Under Drought and Salt Stress

by Haozhe Min, Kexiang Wang, Yao Guo, Junyan Yang, Xuhui Wang, Miao He, Tao Lin, Jiancai Mao and Zhengying Xuan

Biology 2025, 14(7), 891; https://doi.org/10.3390/biology14070891 - 20 Jul 2025

Abstract

Background: The short strand-related sequence (SRS) gene family is a class of plant-specific transcription factors related to a group of genes known as the short internode (SHI) or SRS/STY gene family, which plays important roles in regulating plant growth and development and stress [...] Read more.

Background: The short strand-related sequence (SRS) gene family is a class of plant-specific transcription factors related to a group of genes known as the short internode (SHI) or SRS/STY gene family, which plays important roles in regulating plant growth and development and stress responses. Although the SRS genes have been studied in many plants, in cucurbit crops, they have thus far only been identified in cucumber. Methods: In the Cucurbitaceae database from melon (Cucumis melo), cucumber (Cucumis sativus), watermelon (Citrullus lanatus), bottle gourd (Lagenaria siceraria), wax gourd (Benincasa hispida), moschata pumpkin (Cucurbita moschata), and pumpkin (Cucurbita maxima), a total of 60 SRS genes were identified in seven Cucurbitaceae crops, which were classified into three subfamilies. Results: The same subfamily showed conserved motifs and gene structures. The differences in the number of SRS genes in different Cucurbitaceae crops implied likely gene loss or duplication events during evolution. Analysis of promoter cis-regulatory elements indicated that these SRS genes may be involved in hormone response, growth and development, and biotic and abiotic stress responses in plants. Most of the CmSRS genes in melons were expressed in the roots, with a few expressed in the leaves and ovaries. In addition, CmSRS expression was induced by biotic (wilt and powdery mildew) and abiotic (drought and salt) stresses. Subcellular localization of CmSRS proteins showed predominant expression in the nucleus. Conclusions: A total of 60 Cucurbitaceae SRS genes are present in the genomes of seven Cucurbitaceae crops. These cucurbit SRS genes seem to have maintained similar characteristics and functions during the evolutionary process. These results lay the foundation for the study of biological functions of SRS genes in Cucurbitaceae crops. Full article

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22 pages, 6280 KiB

Open AccessArticle

The V5-Epitope Tag for Cell Engineering and Its Use in Immunohistochemistry and Quantitative Flow Cytometry

by Katja Fritschle, Marion Mielke, Olga J. Seelbach, Ulrike Mühlthaler, Milica Živanić, Tarik Bozoglu, Sarah Dötsch, Linda Warmuth, Dirk H. Busch, Arne Skerra, Christian Kupatt, Wolfgang A. Weber, Richard E. Randall, Katja Steiger and Volker Morath

Biology 2025, 14(7), 890; https://doi.org/10.3390/biology14070890 - 20 Jul 2025

Abstract

Synthetic biology has fundamentally advanced cell engineering and helped to develop effective therapeutics such as chimeric antigen receptor (CAR)-T cells. For these applications, the detection, localization, and quantification of heterologous fusion proteins assembled from interchangeable building blocks is of high importance. The V5 [...] Read more.

Synthetic biology has fundamentally advanced cell engineering and helped to develop effective therapeutics such as chimeric antigen receptor (CAR)-T cells. For these applications, the detection, localization, and quantification of heterologous fusion proteins assembled from interchangeable building blocks is of high importance. The V5 tag, a 14-residue epitope tag, offers promising characteristics for these applications but has only rarely been used in this context. Thus, we have systematically evaluated the murine anti-V5 tag antibody mu_SV5-Pk1 as well as its humanized version, hu_SV5-Pk1, to analyze cells expressing V5-tagged receptors in samples from various in vitro and in vivo experiments. We found that the V5 tag signal on cells is affected by certain fixation and detachment reagents. Immunohistochemistry (IHC) on formalin-fixed paraffin-embedded (FFPE) mouse tissue samples was performed to sensitively detect cells in tissue. We improved IHC by applying the hu_SV5-Pk1 monoclonal antibody (mAb) to avoid cross-reactivity within and unspecific background signals arising on fixed mouse tissue. Conversely, the absence of unspecific binding by the mu_SV5-Pk1 mAb was evaluated on 46 human normal or cancer tissues. Our findings present a robust toolbox for utilizing the V5 tag and cognate antibodies in synthetic biology applications. Full article

(This article belongs to the Section Biochemistry and Molecular Biology)

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25 pages, 2029 KiB

Open AccessArticle

Germination Enhances Phytochemical Profiles of Perilla Seeds and Promotes Hair Growth via 5α-Reductase Inhibition and Growth Factor Pathways

by Anurak Muangsanguan, Warintorn Ruksiriwanich, Pichchapa Linsaenkart, Pipat Tangjaidee, Korawan Sringarm, Chaiwat Arjin, Pornchai Rachtanapun, Sarana Rose Sommano, Korawit Chaisu, Apinya Satsook and Juan Manuel Castagnini

Biology 2025, 14(7), 889; https://doi.org/10.3390/biology14070889 - 20 Jul 2025

Abstract

Seed germination is recognized for enhancing the accumulation of bioactive compounds. Perilla frutescens (L.) Britt., commonly known as perilla seed, is rich in fatty acids that may be beneficial for anti-hair loss. This study investigated the hair regeneration potential of perilla seed extracts—non-germinated [...] Read more.

Seed germination is recognized for enhancing the accumulation of bioactive compounds. Perilla frutescens (L.) Britt., commonly known as perilla seed, is rich in fatty acids that may be beneficial for anti-hair loss. This study investigated the hair regeneration potential of perilla seed extracts—non-germinated (NG-PS) and germinated in distilled water (0 ppm selenium; G0-PS), and germinated with 80 ppm selenium (G80-PS)—obtained from supercritical fluid extraction (SFE) and screw compression (SC). SFE extracts exhibited significantly higher levels of polyphenols, tocopherols, and fatty acids compared to SC extracts. Among the germinated groups, G0-PS showed the highest bioactive compound content and antioxidant capacity. Remarkably, treatment with SFE-G0-PS led to a significant increase in the proliferation and migration of hair follicle cells, reaching 147.21 ± 2.11% (p < 0.05), and resulted in complete wound closure. In addition, its antioxidant and anti-inflammatory properties were reflected by a marked scavenging effect on TBARS (59.62 ± 0.66% of control) and suppressed nitrite amounts (0.44 ± 0.01 µM). Moreover, SFE-G0-PS markedly suppressed SRD5A1-3 gene expression—key regulators in androgenetic alopecia—in both DU-145 and HFDPCs, with approximately 2-fold and 1.5-fold greater inhibition compared to finasteride and minoxidil, respectively. Simultaneously, it upregulated the expression of hair growth-related genes, including CTNNB1, SHH, SMO, GLI1, and VEGF, by approximately 1.5-fold, demonstrating stronger activation than minoxidil. These findings suggest the potential of SFE-G0-PS as a natural therapeutic agent for promoting hair growth and preventing hair loss. Full article

(This article belongs to the Special Issue Nature's Pharmacy: Ethnobotanical Approaches to Medicinal Plant Research for the Discovery and Development of Potential Phytochemicals)

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31 pages, 2257 KiB

Open AccessArticle

Contrasted Ethnobotanical and Literature Knowledge of Anti-Mosquito Plants from Guadeloupe

by Yolène Duchaudé, Laura Brelle, Muriel Sylvestre, Anubis Vega-Rúa and Gerardo Cebrián-Torrejón

Biology 2025, 14(7), 888; https://doi.org/10.3390/biology14070888 - 19 Jul 2025

Abstract

The Aedes aegypti mosquito, vector of dengue, is a major public health threat in the Caribbean. In Guadeloupe, where dengue outbreaks occur frequently, traditional plant-based remedies are part of the local heritage but remain poorly documented. This study aimed to evaluate the anti-mosquito [...] Read more.

The Aedes aegypti mosquito, vector of dengue, is a major public health threat in the Caribbean. In Guadeloupe, where dengue outbreaks occur frequently, traditional plant-based remedies are part of the local heritage but remain poorly documented. This study aimed to evaluate the anti-mosquito potential of 38 Guadeloupean plants through an ethnobotanical survey. A semi-structured online questionnaire was conducted over five months, targeting the plant knowledge of residents. Inclusion/exclusion criteria were applied to identify and validate relevant species. Ethnobotanical indices such as Frequency of Citation (FC), Fidelity Level (FL), and Relative Frequency of Citation (RFC) were calculated. Out of the 38 surveyed plants, 22 were confirmed for their traditional anti-mosquito uses. The most cited species included Cymbopogon citratus (93.3%), Artocarpus altilis (25%), and Pimenta racemosa (18.3%). Comparative analysis with existing literature showed that 12 of these plants had not been previously reported for vector control. This highlights the value of ethnobotanical approaches for discovering alternative, eco-friendly vector control options and the importance of preserving traditional knowledge. The study reveals both the high potential of Guadeloupean flora and the risk of cultural erosion, supporting further research into the bioactive compounds of the most cited species. Full article

(This article belongs to the Special Issue Young Researchers in Plant Sciences)

16 pages, 2100 KiB

Open AccessReview

Romanian Dendrocoelidae Hallez, 1892 (Platyhelminthes, Tricladida, Dendrocoelidae) Revisited: A Tribute to Radu Codreanu and Doina Balcesco

by Anda Felicia Babalean

Biology 2025, 14(7), 887; https://doi.org/10.3390/biology14070887 - 19 Jul 2025

Abstract

This paper presents the current state of knowledge on the Romanian Dendrocoelidae as part of the European/Palearctic Dendrocoelidae, emphasizing the contributions of the Romanian zoologists Radu Codreanu and Doina Balcesco. The main objective of this work was to identify the knowledge gaps for [...] Read more.

This paper presents the current state of knowledge on the Romanian Dendrocoelidae as part of the European/Palearctic Dendrocoelidae, emphasizing the contributions of the Romanian zoologists Radu Codreanu and Doina Balcesco. The main objective of this work was to identify the knowledge gaps for future alignment with current standards. This article presents the species inventory and a short historical overview of the classical phylogenetic system and discusses some morphological characters used in the systematics of the group. This study also analyzes the arguments (and hypotheses) put forward by Codreanu, Balcesco, and other authors regarding the phylogenetic value of various factors, including (a) the position of the oviducts between the male atrium and the bursal canal (typical for Paradendrocoelum); (b) the eyes and the penial flagellum in relation to the palaeogeographical context governed by the Quaternary Glaciation; and (c) the point of view of Codreanu and Balcesco on the origin and composition of the actual Romanian Dendrocoelidae fauna. The major key finding is that the Dendrocoelidae species in Romania should be reinvestigated in an integrative way, and specific research needs and future directions are suggested. Full article

(This article belongs to the Section Zoology)

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16 pages, 3609 KiB

Open AccessArticle

Will Wind Turbines Affect the Distribution of Alashan Ground Squirrel? Insights from Large-Scale Wind Farms in China

by Yuan Wang, Wenbin Yang, Qin Li, Min Zhao, Ying Yang, Xiangfeng Shi, Dazhi Zhang and Guijun Yang

Biology 2025, 14(7), 886; https://doi.org/10.3390/biology14070886 - 19 Jul 2025

Abstract

The wind energy resources in the northwestern desert and semi-desert grassland regions of China are abundant. However, the ramifications of large-scale centralized wind farm operations on terrestrial rodents remain incompletely understood. In May and September 2024, we employed a grid sampling method combined [...] Read more.

The wind energy resources in the northwestern desert and semi-desert grassland regions of China are abundant. However, the ramifications of large-scale centralized wind farm operations on terrestrial rodents remain incompletely understood. In May and September 2024, we employed a grid sampling method combined with burrow counting and kernel density analysis to investigate the spatial distribution of Alashan ground squirrel (Spermophilus alashanicus) burrows in different wind turbine power zones (control, 750 kW, 1500 kW, 2000 kW, and 2500 kW) at the Taiyangshan wind farm in China. Using generalized additive models and structural equation models, we analysed the relationship between burrow spatial distribution and environmental factors. The results revealed no significant linear correlation between burrow density and turbine layout density, but was significantly positively correlated with turbine power (p < 0.05). The highest burrow density was observed in the 2500 kW zone, with values of 24.43 ± 7.18 burrows/hm² in May and 21.29 ± 3.38 burrows/hm² in September (p < 0.05). The squirrels exhibited a tendency to avoid constructing burrows within the rotor sweeping areas of the turbines. The burrow density distribution exhibited a multinuclear clustering pattern in both May and September, with a northwest–southeast spatial orientation. Turbine power, aspect, and plan convexity had significant positive effects on burrow density, whereas vegetation height had a significant negative effect. Moreover, vegetation height indirectly influenced burrow density through its interactions with turbine power and relief degree. Under the combined influence of turbine power, topography, and vegetation, Alashan ground squirrels preferred habitats in low-density, high-power turbine zones with shorter vegetation, sunny slopes, convex landforms, and minimal disturbance. Full article

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31 pages, 2323 KiB

Open AccessArticle

Alpha-Lipoic Acid and Metformin Combination Therapy Synergistically Activate Nrf2-AMPK Signaling Pathways to Ameliorate Cognitive Dysfunction in Type 2 Diabetic Encephalopathy: A Preclinical Study

by Abdulmajeed F. Alrefaei and Mohamed E. Elbeeh

Biology 2025, 14(7), 885; https://doi.org/10.3390/biology14070885 - 18 Jul 2025

Abstract

Diabetic encephalopathy affects over 40% of diabetic patients globally, yet effective treatments remain critically limited. This study investigated the synergistic neuroprotective potential of alpha-lipoic acid (ALA) and metformin through the coordinated activation of Nrf2 and AMPK signaling pathways in type 2 diabetes mellitus [...] Read more.

Diabetic encephalopathy affects over 40% of diabetic patients globally, yet effective treatments remain critically limited. This study investigated the synergistic neuroprotective potential of alpha-lipoic acid (ALA) and metformin through the coordinated activation of Nrf2 and AMPK signaling pathways in type 2 diabetes mellitus (T2DM)-induced encephalopathy. Using a clinically relevant streptozotocin-nicotinamide-induced T2DM rat model, sixty male Sprague–Dawley rats were randomly assigned to five groups: control, diabetic, ALA-treated (300 mg/kg), metformin-treated (50 mg/kg), and combination-treated groups over eight weeks. Combination therapy produced statistically validated synergistic effects with significant interaction terms (p < 0.01) across all evaluated parameters. Nuclear Nrf2 translocation increased 3.9-fold and AMPK phosphorylation rose 3.2-fold compared to monotherapies, surpassing mathematical additivity. Mitochondrial function was remarkably restored, with ATP production increasing to 92% of control levels. Cognitive performance was normalized, with spatial memory approaching control values. Combination index analysis (CI < 1.0) confirmed true synergistic interactions across molecular, cellular, and behavioral endpoints. These findings establish a novel convergent mechanism providing compelling evidence for combination ALA–metformin therapy as an innovative treatment strategy for diabetes-associated neurodegeneration. Full article

(This article belongs to the Special Issue Animal Models of Neurodegenerative Diseases)

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