Long-Term Environmental Hypoxia Exposure and Haematopoietic Prolyl Hydroxylase-1 Deletion Do Not Impact Experimental Crohn’s Like Ileitis
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Environmental Hypoxia Exposure to TNFΔare/+ Mice and Wildtype (WT) Littermates
2.3. Genotyping of the Included Mice
2.4. Blood Composition Analysis
2.5. Histological Assessment of Intestinal Inflammation and Tissue Hypoxia
2.6. Pimonidazole Staining
2.7. RNA Extraction and Quantitative Real-Time Polymerase Chain Reaction
2.8. Assessment of Circulatory Cytokine Expression
2.9. Statistics
3. Results
3.1. Environmental Hypoxia Does Not Influence the General Well-Being of WT Mice, nor the Clinical Course of Inflammation in a Model of Crohn’s Like Ileitis
3.2. Environmental Hypoxia Increases Systemic and Ileal Markers of Hypoxia
3.3. Environmental Hypoxia Induces Systemic Inflammation in WT Mice
3.4. Environmental Hypoxia Induces a ProInflammatory Microenvironment in the Small Intestine of WT Mice
3.5. Environmental Hypoxia Has No Impact on Chronic Ileitis
3.6. Haematopoietic Deletion of Phd1 Does Not Protect against Chronic Ileitis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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De Galan, C.; De Vos, M.; Hindryckx, P.; Laukens, D.; Van Welden, S. Long-Term Environmental Hypoxia Exposure and Haematopoietic Prolyl Hydroxylase-1 Deletion Do Not Impact Experimental Crohn’s Like Ileitis. Biology 2021, 10, 887. https://doi.org/10.3390/biology10090887
De Galan C, De Vos M, Hindryckx P, Laukens D, Van Welden S. Long-Term Environmental Hypoxia Exposure and Haematopoietic Prolyl Hydroxylase-1 Deletion Do Not Impact Experimental Crohn’s Like Ileitis. Biology. 2021; 10(9):887. https://doi.org/10.3390/biology10090887
Chicago/Turabian StyleDe Galan, Cara, Martine De Vos, Pieter Hindryckx, Debby Laukens, and Sophie Van Welden. 2021. "Long-Term Environmental Hypoxia Exposure and Haematopoietic Prolyl Hydroxylase-1 Deletion Do Not Impact Experimental Crohn’s Like Ileitis" Biology 10, no. 9: 887. https://doi.org/10.3390/biology10090887