Quantitative Proteomics Analysis Reveals the Effect of a MarR Family Transcriptional Regulator AHA_2124 on Aeromonas hydrophila
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Bacteria Strains, Plasmids and Cultivation
2.2. Construction of the Aeromonas hydrophila AHA_2124 Deletion Mutant
2.3. Measurement of Hemolytic and Extracellular Proteolytic Activity
2.4. Bacterial Swimming and Swarming Motility Assays
2.5. Protein Sample Preparation
2.6. DIA-Based LC-MS/MS
2.7. Western Blot
2.8. Bioinformatics Analysis
3. Results
3.1. Construction and Phenotype Characterization of the AHA_2124 Deletion Strain in Aeromonas hydrophila
3.2. Proteomics Analysis of Protein Abundance between ΔAHA_2124 and Aeromonas hydrophila WT Strain
3.3. Validation of Proteomics Results
3.4. Functional Analysis of Differentially Abundant Proteins between Δ AHA_2124 and WT Strain
3.5. Protein–Protein Interaction Network of DAPs between WT and ΔAHA_2124 Strain
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primer Name | Primer Sequence (5′→3′) |
---|---|
P1 | CGATCCCAAGCTTCTTCTAGACCGACTGCCTGCTGCGTC |
P2 | TCAGGTCAGAGAAATGTGCGGTTGCG |
P3 | CGCACATTTCTCTGACCTGAACAACTTTGCCACCA |
P4 | CATGAATTCCCGGGAGAGCTCCTGCCGACCATCAGGCAG |
P5 | GGATCTTCCAGAGATGTGCATGTTCAGACAAACGGC |
P6 | CTGCCGTTCGACGATTCATGAGGGTCGCTCGCTAT |
P7 | GGATCTTCCAGAGATGAGCCGCTGTTCGACGCC |
P8 | CTGCCGTTCGACGATTGCCCAGGCCAAGGGCAT |
Accession | Gene | Descriptions | p-Value | log2 (ΔAHA_2124/WT) |
---|---|---|---|---|
A0KI69 | AHA_1431 | Pts system, fructose-specific iiabc component | 1.11 × 10−23 | 10.11 |
A0KFI7 | AHA_0480 | Uncharacterized protein | 6.83 × 10−10 | 7.95 |
A0KK36 | polB | DNA polymerase | 6.49 × 10−9 | 7.64 |
A0KLW8 | AHA_2761 | 4Fe-4S binding domain protein | 1.96 × 10−14 | 7.18 |
A0KEP9 | ubiA | 4-hydroxybenzoate octaprenyltransferase | 9.89 × 10−15 | 6.94 |
A0KQJ3 | AHA_4120 | Probable nitrite transporter | 2.23 × 10−11 | 5.90 |
A0KFS4 | AHA_0567 | Heat shock protein 15 | 7.26 × 10−8 | 3.78 |
A0KK31 | lpxM | Lipid A biosynthesis myristoyltransferase | 2.49 × 10−6 | 3.77 |
A0KM07 | AHA_2803 | Uncharacterized protein | 7.30 × 10−4 | 3.67 |
A0KLP4 | AHA_2687 | Microbial serine proteinase | 2.54 × 10−3 | 3.27 |
A0KQ31 | AHA_3948 | Uncharacterized protein | 1.55 × 10−9 | 2.72 |
A0KL71 | selB | Selenocysteine-specific translation elongation factor | 1.49 × 10−6 | 2.58 |
A0KL58 | fdhA | Formate dehydrogenase, alpha subunit, selenocysteine-containing | 4.54 × 10−4 | 2.55 |
A0KLH0 | AHA_2612 | Oligopeptide ABC transporter, permease protein OppB | 1.80 × 10−10 | −7.84 |
A0KKJ5 | AHA_2273 | Phospholipase, patatin family | 7.18 × 10−7 | −8.19 |
A0KEY3 | AHA_0271 | Uncharacterized protein | 6.46 × 10−13 | −8.39 |
A0KQT0 | AHA_4209 | Phosphate acyltransferase family protein | 1.13 × 10−5 | −8.55 |
A0KIW0 | AHA_1675 | Uncharacterized protein | 3.20 × 10−5 | −8.85 |
A0KLV6 | AHA_2749 | Putative membrane protein | 6.70 × 10−3 | −9.41 |
A0KFG4 | AHA_0456 | Acetyltransferase | 2.07 × 10−8 | −9.55 |
A0KK46 | AHA_2123 | Uncharacterized protein | 2.83 × 10−12 | −9.69 |
A0KLG9 | AHA_2611 | Oligopeptide ABC transporter, permease protein OppC | 1.44 × 10−9 | −9.72 |
A0KLT3 | AHA_2726 | Na+/H+ antiporter family protein | 4.95 × 10−5 | −12.40 |
A0KLQ5 | AHA_2698 | Diguanylate cyclase/phosphodiesterase domain 1 | 1.25 × 10−11 | −13.02 |
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Li, Z.; Li, W.; Lu, J.; Liu, Z.; Lin, X.; Liu, Y. Quantitative Proteomics Analysis Reveals the Effect of a MarR Family Transcriptional Regulator AHA_2124 on Aeromonas hydrophila. Biology 2023, 12, 1473. https://doi.org/10.3390/biology12121473
Li Z, Li W, Lu J, Liu Z, Lin X, Liu Y. Quantitative Proteomics Analysis Reveals the Effect of a MarR Family Transcriptional Regulator AHA_2124 on Aeromonas hydrophila. Biology. 2023; 12(12):1473. https://doi.org/10.3390/biology12121473
Chicago/Turabian StyleLi, Zhen, Wanxin Li, Jinlian Lu, Ziqiu Liu, Xiangmin Lin, and Yanling Liu. 2023. "Quantitative Proteomics Analysis Reveals the Effect of a MarR Family Transcriptional Regulator AHA_2124 on Aeromonas hydrophila" Biology 12, no. 12: 1473. https://doi.org/10.3390/biology12121473