Cumulus Cell and Oocyte Gene Expression in Prepubertal Gilts and Sows Identifies Cumulus Cells as a Prime Informative Parameter of Oocyte Quality
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Chemicals and Media
2.2. Animal Material and Ethics
2.3. Experimental Design
2.4. Selection of Target Genes
2.5. Cumulus–Oocyte Complex Collection and In Vitro Maturation
2.6. RNA Extraction and cDNA Synthesis
2.7. Quantitative PCR
2.8. Statistical Analysis
3. Results
3.1. Gene Expression in Porcine Cumulus Cells
3.2. Gene Expression in Porcine Oocytes
3.3. The Rate of the Pentose Phosphate Pathway Relative to Glycolysis
4. Discussion
4.1. Glucose Metabolism
4.2. Fatty Acid Oxidation
4.3. L-Carnitine Biosynthesis
4.4. Apoptosis
4.5. Gene Expression of PTGS2, GHR and EGFR
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
Gene Symbol | TaqMan® Assay | Accession Numbers of Transcript Variants |
---|---|---|
G6PD | Ss02690824_g1 | XM_021080744.1 (X1), XM_003360515.5 (X2) |
ALDOA | Ss06920688_m1 | XM_021087995.1 (X1), XM_021087996.1 (X2), XM_021087997.1 (X3), XM_021087998.1 (X4), XM_021088000.1 (X6), XM_021088001.1 (X7), XM_021088002.1 (X8), XM_021088004.1 (X9) |
TMLHE | Ss06886117_m1 | XM_003135511.4 (X1), XM_021080447.1 (X2), XM_021080448.1 (X3), XM_021080450.1 (X4), XM_005674022.3 (X5) |
BBOX1 | Ss06906097_m1 | XM_021083234.1 (X1), XM_021083235.1 (X2), XM_021083236.1 (X3) |
GHR | Ss03383662_u1 | XM_021076567.1 (X1), XM_013990636.2 (X2), XM_021076568.1 (X3), XM_021076569.1 (X4), XM_021076570.1 (X5), XM_021076571.1 (X6), XM_021076572.1 (X7), XM_021076573.1 (X8), XM_021076574.1 (X9), XM_013990637.2 (X10), XM_021076575.1 (X11) |
BAX | Ss03375842_u1 | XM_003127290.5 (X1), XM_013998624.2 (X2) |
BCL2L1 | Ss03383783_s1 | XM_021077292.1 (X1), XM_021077293.1 (X2), XM_021077294.1 (X3), XM_021077295.1 (X4), XM_021077296.1 (X5), XM_021077297.1 (X6), XM_021077298.1 (X7) |
ACTB | Ss03376563_uH | XM_003124280.5 (X1), XM_021086047.1 (X2) |
GAPDH | Ss03375629_u1 | XM_021091114.1 (X2) |
HMBS | Ss03388782_g1 | XM_021102144.1 (X1), XM_021102146.1 (X2), XM_021102147.1 (X3), XM_021102148.1 (X4) |
HPRT1 | Ss03388274_m1 | XM_021079503.1 (X1), XM_021079504.1 (X2) |
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Gene Symbol | Function | Accession Number | TaqMan® Assay |
---|---|---|---|
G6PD | Pentose phosphate pathway | XM_003360515.5 †† | Ss02690824_g1 |
PFKP | Glycolysis | XM_021065066.1 | Ss06887532_m1 |
ALDOA | Glycolysis | XM_021087995.1 †† | Ss06920688_m1 |
TMLHE | Carnitine biosynthesis | XM_003135511.4 †† | Ss06886117_m1 |
BBOX1 | Carnitine biosynthesis | XM_021083234.1 †† | Ss06906097_m1 |
CPT2 | Fatty acid metabolism | NM_001246243.1 | Ss04322743_m1 |
GHR † | Growth hormone receptor | NM_214254.2 †† | Ss03383662_u1 |
EGFR † | Epidermal growth factor receptor | NM_214007.1 | Ss03393423_u1 |
PTGS2 † | Cumulus cell expansion and function | NM_214321.1 | Ss03394694_m1 |
BAX † | Pro-apoptotic | XM_003127290.5 †† | Ss03375842_u1 |
BCL2L1 † | Anti-apoptotic | NM_214285.1 †† | Ss03383783_s1 |
ACTB | Cytoskeletal structural protein | XM_003124280.5 †† | Ss03376563_uH |
GAPDH | Glycolysis | NM_001206359.1 †† | Ss03375629_u1 |
HMBS | Heme biosynthesis | NM_001097412.1 †† | Ss03388782_g1 |
HPRT1 | Recycling of purines | NM_001032376.2 | Ss03388274_m1 |
YWHAG | Signal transduction | XM_005661962.3 | Ss06938931_s1 |
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Haug, L.M.; Wilson, R.C.; Gaustad, A.H.; Jochems, R.; Kommisrud, E.; Grindflek, E.; Alm-Kristiansen, A.H. Cumulus Cell and Oocyte Gene Expression in Prepubertal Gilts and Sows Identifies Cumulus Cells as a Prime Informative Parameter of Oocyte Quality. Biology 2023, 12, 1484. https://doi.org/10.3390/biology12121484
Haug LM, Wilson RC, Gaustad AH, Jochems R, Kommisrud E, Grindflek E, Alm-Kristiansen AH. Cumulus Cell and Oocyte Gene Expression in Prepubertal Gilts and Sows Identifies Cumulus Cells as a Prime Informative Parameter of Oocyte Quality. Biology. 2023; 12(12):1484. https://doi.org/10.3390/biology12121484
Chicago/Turabian StyleHaug, Linda Marijke, Robert C. Wilson, Ann Helen Gaustad, Reina Jochems, Elisabeth Kommisrud, Eli Grindflek, and Anne Hege Alm-Kristiansen. 2023. "Cumulus Cell and Oocyte Gene Expression in Prepubertal Gilts and Sows Identifies Cumulus Cells as a Prime Informative Parameter of Oocyte Quality" Biology 12, no. 12: 1484. https://doi.org/10.3390/biology12121484