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Open AccessReview
The Utility of the Koala Scat: A Scoping Review
by
Stephen D. Johnston
Stephen D. Johnston
Prof. Stephen Johnston is a Reader in Wildlife Reproduction at the School of Environment at the of a [...]
Prof. Stephen Johnston is a Reader in Wildlife Reproduction at the School of Environment at the University of Queensland. Prof. Johnston is a zoologist specialising in the area of reproductive biology in a broad diversity of species ranging from prawns to tigers but with a major focus on Australian mammals. He was the first person in the world to produce a pouch young following artificial insemination in a marsupial, a task that he and his colleagues have now carried out successfully in the koala, a total of 34 times. He is also a specialist in the cryopreservation of marsupial spermatozoa and
the assessment of sperm DNA fragmentation, including human and domestic animal
spermatozoa. Prof. Johnston’s recent research interests and grant success include studies aimed at a better understanding of the effect of chlamydia on male koala reproduction, genetic and reproductive management of koalas, wombat captive reproduction, echidna captive breeding, and crocodile artificial
insemination. He was recently elected a Fellow of the Society for Reproductive Biology (2019).
1,2,*
,
Lyndal Hulse
Lyndal Hulse
Dr. Lyndal S Hulse is a Postdoctoral Research Fellow at The
University of Queensland with expertise [...]
Dr. Lyndal S Hulse is a Postdoctoral Research Fellow at The
University of Queensland with expertise in wildlife genomics and genetics.
Lyndal completed her PhD in 2021 and examined the effect of Chlamydia on male
koala fertility. Previous to being employed at The University of Queensland,
Lyndal was employed as a senior scientist and project manager within the
private sector veterinary pathology and biotechnology industries. She has
undergraduate degrees from The University of Queensland and a postgraduate
degree Master of Science in Agriculture from the University of New England
specialising in molecular biology and quantitative genetics. Lyndal has
extensive molecular and animal genetics experience and specializes in wildlife
genomics and the development and implementation of molecular diagnostic assays
within various animal industries.
1,2
,
Tamara Keeley
Tamara Keeley
Dr. Tamara Keeley received her Ph.D. in Philosophy from the
University of Sydney. Now she is a at a [...]
Dr. Tamara Keeley received her Ph.D. in Philosophy from the
University of Sydney. Now she is a Senior Lecturer at the University of Queensland,
School of Agriculture and Food Science, and a biologist, specializing in the
areas of Endocrinology, Reproductive Biology, Physiology, Captive Management,
Conservation Science, Behaviour, and Welfare. She has experience working with a
large variety of species ranging from livestock to endangered species (both in
situ and ex situ); including mammals, marsupials, amphibians, and reptiles.
Over the last decade and a half, Dr. Keeley has been involved in a wide range
of management and research-related collaborations and projects through which
she has gained experience and knowledge in the areas of animal management and
husbandry, reproductive management, and assisted reproductive technology,
animal physiology, animal behavior and welfare, genetics, ecology and wildlife
recovery and conservation programs. Dr. Keeley is known for her expert
knowledge of non-invasive hormone analysis techniques and their validation and
application to wildlife, companion, laboratory, and production animals for the
study of reproduction, welfare, and health.
1
,
Albano Mucci
Albano Mucci 1,
Jennifer Seddon
Jennifer Seddon
Professor Jenny Seddon is currently the Deputy Vice Chancellor Research at James Cook University, of [...]
Professor Jenny Seddon is currently the Deputy Vice Chancellor Research at James Cook University, Australia. Before her current appointment, she held the role of Associate
Dean Research and Deputy Executive Dean for the Faculty of Science at the
University of Queensland. Prof Seddon's research is in animal genetics and genomics, with much of her work in the areas of phylogeography and
population genomics to develop multidisciplinary, practical solutions
for the conservation of wildlife.
2,3
and
Sam Maynard
Sam Maynard 4
1
School of Environment, The University of Queensland, Gatton 4343, Australia
2
School of Veterinary Science, The University of Queensland, Gatton 4343, Australia
3
Research Division, James Cook University, Townsville 4811, Australia
4
Saunders Havill Group, Bowen Hills 4006, Australia
*
Author to whom correspondence should be addressed.
Submission received: 27 February 2024
/
Revised: 21 June 2024
/
Accepted: 5 July 2024
/
Published: 15 July 2024
Simple Summary
This review reports on the current and potential utility of the “koala scat” sample to provide a range of ecological and physiological assessments both at the population and individual animal level and do so in a non-invasive manner. DNA recovered from the scat sample provides useful information on koala distribution, diet, genetics and disease, whereas hormone metabolites can inform physiology. While there are still limitations with respect to the decay of quality DNA (host, microbiome, and pathogen) over time related to climate and sample handling, some of these issues can be overcome with timely sample collection. Other current limitations include an inability to detect and quantify particular hormone metabolites such as oestrogens and/or an appropriate biological interpretation of glucocorticoid metabolite secretion when measured in the faecal sample.
Abstract
The use of samples or scats to provide important ecological, genetic, disease and physiology details on free-range populations is gaining popularity as an alternative non-invasive methodology. Koala populations in SE Queensland and NSW have recently been listed as endangered and continue to face anthropomorphic and stochastic environmental impacts that could potentially lead to their extinction. This scoping review examines the current and potential utility of the koala scat to contribute data relevant to the assessment of koala conservation status and decision making. Although we demonstrate that there is great potential for this methodology in providing details for both individual wild animal and population biology (distribution, abundance, sex ratio, immigration/emigration, genetic diversity, evolutionary significant unit, disease epidemiology, nutrition, reproductive status and stress physiology), the calibre of this information is likely to be a function of the quality of the scat that is sampled.
Share and Cite
MDPI and ACS Style
Johnston, S.D.; Hulse, L.; Keeley, T.; Mucci, A.; Seddon, J.; Maynard, S.
The Utility of the Koala Scat: A Scoping Review. Biology 2024, 13, 523.
https://doi.org/10.3390/biology13070523
AMA Style
Johnston SD, Hulse L, Keeley T, Mucci A, Seddon J, Maynard S.
The Utility of the Koala Scat: A Scoping Review. Biology. 2024; 13(7):523.
https://doi.org/10.3390/biology13070523
Chicago/Turabian Style
Johnston, Stephen D., Lyndal Hulse, Tamara Keeley, Albano Mucci, Jennifer Seddon, and Sam Maynard.
2024. "The Utility of the Koala Scat: A Scoping Review" Biology 13, no. 7: 523.
https://doi.org/10.3390/biology13070523
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