Methanogenic Community Characteristics and Its Influencing Factors in Reservoir Sediments on the Northeastern Qinghai Plateau
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sampling Site
2.2. Sample Collection and Preservation
2.3. Environmental Data Collection and Determination
2.4. DNA Extraction and PCR Amplification of Methanogens
2.5. Statistical Analysis
3. Results
3.1. Composition of Methanogens
3.2. Diversity of Methanogens
3.3. Co-Occurrence Network of Methanogens
3.4. FAPROTAX Ecological Function Prediction of Methanogens
4. Discussion
4.1. Temporal and Spatial Characteristics of Methanogenic Composition and Diversity
4.2. Influencing Factors on the Composition and Diversity of Methanogens
4.3. Hydrogenotrophic Methanogenesis Was the Dominant Pathway
5. Conclusions
- (1)
- The dominant genus of methanogens in the reservoir sediments on the northeastern Qinghai Plateau are Methanobacterium (28.87%) and Methanosarcina (21.67%). The hydrogenotrophic pathway using CO2/H2 as substrates predominates in the production of methane in reservoir sediments.
- (2)
- The α-diversity of methanogenic communities is higher during the wet season compared to the dry season (p > 0.05). Additionally, the diversity of methanogens is greater in large reservoirs than in small reservoirs (p < 0.05). β-diversity exhibits significant differences between different seasons and river basins (p < 0.05). Furthermore, temperature and pH have a significant impact on the composition and diversity of methanogenic communities in the reservoir sediments on the northeastern Qinghai Plateau (p < 0.05).
- (3)
- The network stability of methanogens in the reservoir sediments on the northeastern Qinghai Plateau is robust, as indicated by a modularity index of 0.69. The main function within this network is cooperation, with a high positive correlation edge number of 99.82%.
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Liu, Z.; Mao, X.; Wu, Y.; Xia, L.; Yu, H.; Tang, W.; Qi, Y.; Zhang, Z.; Xiao, F.; Ji, H. Methanogenic Community Characteristics and Its Influencing Factors in Reservoir Sediments on the Northeastern Qinghai Plateau. Biology 2024, 13, 615. https://doi.org/10.3390/biology13080615
Liu Z, Mao X, Wu Y, Xia L, Yu H, Tang W, Qi Y, Zhang Z, Xiao F, Ji H. Methanogenic Community Characteristics and Its Influencing Factors in Reservoir Sediments on the Northeastern Qinghai Plateau. Biology. 2024; 13(8):615. https://doi.org/10.3390/biology13080615
Chicago/Turabian StyleLiu, Zebi, Xufeng Mao, Yi Wu, Liang Xia, Hongyan Yu, Wenjia Tang, Yanhong Qi, Ziping Zhang, Feng Xiao, and Haichuan Ji. 2024. "Methanogenic Community Characteristics and Its Influencing Factors in Reservoir Sediments on the Northeastern Qinghai Plateau" Biology 13, no. 8: 615. https://doi.org/10.3390/biology13080615