Factors Affecting Cell Viability during the Enzymatic Dissociation of Human Endocrine Tumor Tissues
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Tissue Sampling
2.2. Histopathological Examination
2.3. Enzymatic Dissociation
2.4. Statistical Data Analysis
3. Results and Discussion
3.1. Influence of Morphological Features on the Dissociation Process and Cell Viability
3.2. Influence of Enzymatic Activity on Cell Viability
3.3. Influence Dissociation Time on Cell Viability
3.4. Influence of Additional Post-Dissociation Purification Procedures on Cell Viability
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Shcherbakova, A.; Utkina, M.; Valyaeva, A.; Pachuashvili, N.; Bondarenko, E.; Urusova, L.; Popov, S.; Mokrysheva, N. Factors Affecting Cell Viability during the Enzymatic Dissociation of Human Endocrine Tumor Tissues. Biology 2024, 13, 665. https://doi.org/10.3390/biology13090665
Shcherbakova A, Utkina M, Valyaeva A, Pachuashvili N, Bondarenko E, Urusova L, Popov S, Mokrysheva N. Factors Affecting Cell Viability during the Enzymatic Dissociation of Human Endocrine Tumor Tissues. Biology. 2024; 13(9):665. https://doi.org/10.3390/biology13090665
Chicago/Turabian StyleShcherbakova, Anastasia, Marina Utkina, Anna Valyaeva, Nano Pachuashvili, Ekaterina Bondarenko, Liliya Urusova, Sergey Popov, and Natalya Mokrysheva. 2024. "Factors Affecting Cell Viability during the Enzymatic Dissociation of Human Endocrine Tumor Tissues" Biology 13, no. 9: 665. https://doi.org/10.3390/biology13090665