Next Article in Journal
Cosmetology in the Era of Artificial Intelligence
Previous Article in Journal
Positive Effect of Peptides Obtained from Nile Tilapia (Oreochromis niloticus) on Inflammation Regulation and Wound Healing
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Protective Effects of Recombined Mussel Adhesive Protein against AD Skin Inflammation in Mice

1
Department of Pharmacology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China
2
Health Science Center, Department of Pharmacology, Hubei Minzu University, Enshi 445000, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Cosmetics 2024, 11(4), 134; https://doi.org/10.3390/cosmetics11040134
Submission received: 23 May 2024 / Revised: 22 July 2024 / Accepted: 1 August 2024 / Published: 9 August 2024
(This article belongs to the Section Cosmetic Dermatology)

Abstract

(1) Background: Atopic dermatitis (AD) is characterized as a chronic inflammatory skin disease with a significant incidence rate. The pathophysiological mechanisms underlying AD remain incompletely understood. However, extensive research demonstrates that a complex interplay among genetic, immune, and environmental factors contributes to the disruption of skin barrier function. Inflammation is identified as one of the pathological mechanisms in AD. Recombined mussel adhesive protein exhibits anti-inflammatory properties. However, recombinant mussel adhesive protein has been used less frequently for AD, so we explored the therapeutic effect of recombinant mussel adhesive protein for AD and the potential mechanism. (2) Methods: We established a mice model of AD in vivo and an LPS-induced inflammation model in HaCaT cells in vitro. Through assessment of skin lesion scores, itch frequency, transepidermal water loss, skin microcirculation, HE staining, Elisa assays for IL-6, IL-12, IL-13, IL-4, IL-5, IFN-γ, IgE, and TNF-α, immunohistochemical staining for filaggrin and CK14, Masson staining, and Western blot analysis of NF-κB p65, P-P65, Keap1, and Nrf2, the effects of recombined mussel adhesive protein on AD symptoms, pathology, inflammation, and its mechanisms are investigated. (3) Results: The recombined mussel adhesive protein significantly improved the compromised skin barrier, reduced scratching frequency in mice, decreased transepidermal water loss, and lowered the expression of inflammatory factors, thus ameliorating skin inflammation damage. Mechanistically, recombined mussel adhesive protein downregulated the expression of P-p65/p65 and Keap1 while upregulating the level of Nrf2. (4) Conclusions: Overall, our results demonstrate the effectiveness of recombined mussel adhesive protein in attenuating DNFB-induced AD by inhibiting NF-κB and activating the Keap1/Nrf2 signaling pathway. Thus, recombined mussel adhesive protein is a promising therapeutic candidate for the treatment of AD.
Keywords: atopic dermatitis; recombined mussel adhesive protein; inflammation; NF-κB; Keap1/Nrf2 atopic dermatitis; recombined mussel adhesive protein; inflammation; NF-κB; Keap1/Nrf2

Share and Cite

MDPI and ACS Style

Wu, Y.; Li, F.; Gong, Y.; Wan, X.; Zhou, L.-M. Protective Effects of Recombined Mussel Adhesive Protein against AD Skin Inflammation in Mice. Cosmetics 2024, 11, 134. https://doi.org/10.3390/cosmetics11040134

AMA Style

Wu Y, Li F, Gong Y, Wan X, Zhou L-M. Protective Effects of Recombined Mussel Adhesive Protein against AD Skin Inflammation in Mice. Cosmetics. 2024; 11(4):134. https://doi.org/10.3390/cosmetics11040134

Chicago/Turabian Style

Wu, Yu, Feng Li, Yan Gong, Xing Wan, and Li-Ming Zhou. 2024. "Protective Effects of Recombined Mussel Adhesive Protein against AD Skin Inflammation in Mice" Cosmetics 11, no. 4: 134. https://doi.org/10.3390/cosmetics11040134

APA Style

Wu, Y., Li, F., Gong, Y., Wan, X., & Zhou, L.-M. (2024). Protective Effects of Recombined Mussel Adhesive Protein against AD Skin Inflammation in Mice. Cosmetics, 11(4), 134. https://doi.org/10.3390/cosmetics11040134

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop