Method Development and Validation of Almotriptan in Human Plasma by HPLC Tandem Mass Spectrometry: Application to Pharmacokinetic Study

RAVIKUMAR, Konda; CHANDU, Babu Rao; CHALLA, Balasekhara Reddy; CHANDRASEKHAR, Kottapalli B.

doi:10.3797/scipharm.1112-01

Open AccessArticle

Method Development and Validation of Almotriptan in Human Plasma by HPLC Tandem Mass Spectrometry: Application to Pharmacokinetic Study

by

Konda RAVIKUMAR

^1,2,

Babu Rao CHANDU

³,

Balasekhara Reddy CHALLA

^4,* and

Kottapalli B. CHANDRASEKHAR

²

¹

Hindu college of Pharmacy, Amaravathi Road, Guntur, Andhrapradesh, 522002, India

²

Jawaharlal Nehru Technological University, Anantapur, 515002, India

³

Donbosco college of Pharmacy, Pulladigunta, Guntur, 522201, India

⁴

Nirmala college of Pharmacy, Madras road, Kadapa, Andhrapradesh, 516002, India

^*

Author to whom correspondence should be addressed.

Sci. Pharm. 2012, 80(2), 367-378; https://doi.org/10.3797/scipharm.1112-01

Submission received: 1 December 2011 / Accepted: 27 February 2012 / Published: 27 February 2012

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Abstract

A simple, sensitive and selective method has been developed for quantification of Almotriptan (AL) in human plasma using Almotriptan-d6 (ALD6) as an internal standard. Almotriptan and Almotriptan-d6 were detected with proton adducts at m/z 336.1→201.1 and 342.2→207.2 in multiple reaction monitoring (MRM) positive mode, respectively. The method was linear over a concentration range of 0.5–150.0 ng/mL. The limit of detection (LOD) and limit of quantification (LOQ) for Almotriptan were 0.2 pg/mL and 0.5 ng/mL, respectively. Liquid-liquid extraction was used followed by MS/MS (ion spray). The method was shown to be precise with an average within-run and between-run variation of 0.68 to 2.78% and 0.57 to 0.86%, respectively. The average within-run and between-run accuracy of the method throughout its linear range was 98.94 to 102.64% and 99.43 to 101.44%, respectively. The mean recovery of drug and internal standard from human plasma was 92.12 ± 4.32% and 89.62 ± 6.32%. It can be applied for clinical and pharmacokinetic studies.

Keywords: HPLC; MS/MS; Almotriptan; Human plasma; Pharmacokinetic study; LLE

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MDPI and ACS Style

RAVIKUMAR, K.; CHANDU, B.R.; CHALLA, B.R.; CHANDRASEKHAR, K.B. Method Development and Validation of Almotriptan in Human Plasma by HPLC Tandem Mass Spectrometry: Application to Pharmacokinetic Study. Sci. Pharm. 2012, 80, 367-378. https://doi.org/10.3797/scipharm.1112-01

AMA Style

RAVIKUMAR K, CHANDU BR, CHALLA BR, CHANDRASEKHAR KB. Method Development and Validation of Almotriptan in Human Plasma by HPLC Tandem Mass Spectrometry: Application to Pharmacokinetic Study. Scientia Pharmaceutica. 2012; 80(2):367-378. https://doi.org/10.3797/scipharm.1112-01

Chicago/Turabian Style

RAVIKUMAR, Konda, Babu Rao CHANDU, Balasekhara Reddy CHALLA, and Kottapalli B. CHANDRASEKHAR. 2012. "Method Development and Validation of Almotriptan in Human Plasma by HPLC Tandem Mass Spectrometry: Application to Pharmacokinetic Study" Scientia Pharmaceutica 80, no. 2: 367-378. https://doi.org/10.3797/scipharm.1112-01

APA Style

RAVIKUMAR, K., CHANDU, B. R., CHALLA, B. R., & CHANDRASEKHAR, K. B. (2012). Method Development and Validation of Almotriptan in Human Plasma by HPLC Tandem Mass Spectrometry: Application to Pharmacokinetic Study. Scientia Pharmaceutica, 80(2), 367-378. https://doi.org/10.3797/scipharm.1112-01

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Method Development and Validation of Almotriptan in Human Plasma by HPLC Tandem Mass Spectrometry: Application to Pharmacokinetic Study

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