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Volume 80
Issue 2
10.3797/scipharm.1109-04
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Article

Characterization of Constituents and Anthelmintic Properties of Hagenia abyssinica

by
Henrieke THOMSEN
1,
Katrin REIDER
1,
Katrin FRANKE
1,
Ludger A. WESSJOHANN
1,
Jennifer KEISER
2,3,
Ermias DAGNE
4 and
Norbert ARNOLD
1,*
1
Leibniz Institute of Plant Biochemistry, Weinberg 3, D-06120 Halle (Saale), Germany
2
Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland
3
University of Basel, P.O. Box, CH-4003 Basel, Switzerland
4
University of Addis Ababa, P.O. Box 30270, Addis Ababa, Ethiopia
*
Author to whom correspondence should be addressed.
Sci. Pharm. 2012, 80(2), 433-446; https://doi.org/10.3797/scipharm.1109-04
Submission received: 16 September 2011 / Accepted: 2 March 2012 / Published: 2 March 2012
Versions Notes

Abstract

The dried female flowers of Hagenia abyssinica (Bruce) J. F. Gmel. (Rosaceae) are traditionally used as an anthelmintic remedy in Ethiopia and formerly were incorporated into the European Pharmacopoeia. One-, two- and tricyclic phloroglucinol derivatives (kosins) were suggested to be the active principles. However, polar constituents may also contribute to the activity. Therefore, we investigated for the first time the polar constituents. We isolated typical Rosaceae constituents such as quercetin 3-O-β-glucuronide, quercetin 3-O-β-glucoside and rutin. Polar kosin glycosides or derivatives could not be detected.
The anthelmintic activity of fractions of different polarity were tested against the blood fluke Schistosoma mansoni, the liver flukes Clonorchis sinensis and Fasciola hepatica and the intestinal fluke Echinostoma caproni. The anthelmintic activity decreased with increasing polarity of the tested fractions. ESI-MS investigations indicated the predominant occurrence of kosins in the active fractions.
Using the anthelmintic active extracts of Hagenia abyssinica we developed a simple, inexpensive bioassay against the non-parasitic nematode Caenorhabditis elegans, which can be used as an initial screening procedure for anthelmintic properties of crude extracts of plants or fungi. The anthelmintic activity of test extracts against the model organism was determined in a microtiter plate assay by enumeration of living and dead nematodes under a microscope.
Keywords: Chaenorhabditis elegans; Trematodes; Bioassay; Kosso; Bioactivity; Kosins Chaenorhabditis elegans; Trematodes; Bioassay; Kosso; Bioactivity; Kosins

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MDPI and ACS Style

THOMSEN, H.; REIDER, K.; FRANKE, K.; WESSJOHANN, L.A.; KEISER, J.; DAGNE, E.; ARNOLD, N. Characterization of Constituents and Anthelmintic Properties of Hagenia abyssinica. Sci. Pharm. 2012, 80, 433-446. https://doi.org/10.3797/scipharm.1109-04

AMA Style

THOMSEN H, REIDER K, FRANKE K, WESSJOHANN LA, KEISER J, DAGNE E, ARNOLD N. Characterization of Constituents and Anthelmintic Properties of Hagenia abyssinica. Scientia Pharmaceutica. 2012; 80(2):433-446. https://doi.org/10.3797/scipharm.1109-04

Chicago/Turabian Style

THOMSEN, Henrieke, Katrin REIDER, Katrin FRANKE, Ludger A. WESSJOHANN, Jennifer KEISER, Ermias DAGNE, and Norbert ARNOLD. 2012. "Characterization of Constituents and Anthelmintic Properties of Hagenia abyssinica" Scientia Pharmaceutica 80, no. 2: 433-446. https://doi.org/10.3797/scipharm.1109-04

APA Style

THOMSEN, H., REIDER, K., FRANKE, K., WESSJOHANN, L. A., KEISER, J., DAGNE, E., & ARNOLD, N. (2012). Characterization of Constituents and Anthelmintic Properties of Hagenia abyssinica. Scientia Pharmaceutica, 80(2), 433-446. https://doi.org/10.3797/scipharm.1109-04

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