Application of CoLD-CoP to Detecting Competitively and Cooperatively Binding Ligands
Abstract
:1. Introduction
2. Materials and Methods
3. Results
3.1. Detecting Competitive Binding to Tyrosinase
3.2. Detecting Cooperative Binding to Lysozyme
3.3. Validation of Cooperative Binding
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Mixture | Compound | Concentration (mM 1) |
---|---|---|
Tyrosinase Ligand Only | GlcNAc | 2 |
(TL) | Salicylic Acid | 3 |
Tartrate | 2 | |
Thiamine | 2 | |
Tris 2 | 10 | |
Tryptamine | 3 | |
ligand+protein | TL—Salicylic Acid 3 | (as above) |
(TLP) | Salicylic Acid | 1 |
Mushroom Tyrosinase | 4 μM | |
ligand+protein+known ligand | TLP | (as above) |
(TLPK) | 4-HCCA 4 | 8 |
Lysozyme ligand only | Caffeine | 50 |
(LL) | Citrate | 50 |
Imidazole | 100 | |
Tartrate | 50 | |
Tris 2 | 50 | |
Tryptamine | 50 | |
ligand+protein | LL | (as above) |
(LLP) | Hen Egg White Lysozyme | 1 |
ligand+protein+known ligand | LLP | (as above) |
(LLPK) | GlcNAc | 300 |
Parameters | GlcNAc 1 | Tris 1 | Ternary Complex 1 | Ternary Complex— (GlcNAc + Tris) 1 |
---|---|---|---|---|
PB1 | 5.41 | −5.32 | −5.34 | −5.43 |
PB3 | −6.89 | −16.99 | −27.33 | −3.45 |
PB4 | −17.16 | −25.67 | −42.35 | 0.48 |
GB1 | −23.13 | −28.59 | −49.64 | 2.08 |
GB2 | −25.42 | −27.1 | −55.25 | −2.73 |
GB5 2 | −29 | −28.05 | −59.96 | −2.91 |
GB6 | −20.25 | −26.19 | −50.8 | −4.36 |
GB7 2 | −34.67 | −26.31 | −68.03 | −7.05 |
GB8 2,3 | −21.77 | −20.27 | −45.05 | −3.01 |
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Patnala, S.V.; Robles, R.; Snyder, D.A. Application of CoLD-CoP to Detecting Competitively and Cooperatively Binding Ligands. Biomolecules 2024, 14, 1136. https://doi.org/10.3390/biom14091136
Patnala SV, Robles R, Snyder DA. Application of CoLD-CoP to Detecting Competitively and Cooperatively Binding Ligands. Biomolecules. 2024; 14(9):1136. https://doi.org/10.3390/biom14091136
Chicago/Turabian StylePatnala, Shiva V., Roberto Robles, and David A. Snyder. 2024. "Application of CoLD-CoP to Detecting Competitively and Cooperatively Binding Ligands" Biomolecules 14, no. 9: 1136. https://doi.org/10.3390/biom14091136