The Hippo Pathway Effectors YAP/TAZ Are Essential for Mineralized Tissue Homeostasis in the Alveolar Bone/Periodontal Complex
Abstract
:1. Introduction
2. Methods
2.1. Animal Model
2.2. Unopposed Molar Model
2.3. Isolation of Primary Periodontal Ligament Cells
2.4. Radiographic Imaging
2.5. Histology
2.6. Application of Mechanical Stress on Cells
2.7. siRNA Mediated Knockdown of YAP
2.8. Immunofluorescence Microscopy
2.9. RNA Extraction and Real-Time Quantitative PCR
2.10. Statistical Analysis
3. Results
3.1. Reduced Mineralization-Related Gene Expression, Incomplete Cellular Cementum Mineralization, and 3rd Molar Tooth Row Misalignment in Wnt1cre/YAP Flox/TAZ Flox Mice
3.2. Cyclic Strain Increased Nuclear YAP, and the Proteoglycan Agrin Partially Rescued the Effect of YAP Knockdown on Nuclear YAP Expression
3.3. Significant Reduction in Mineralization-Related Gene Expression following YAP1 Knockdown, and Partial Rescue with the Agrin Proteoglycan
3.4. Reduced Mineralization-Related Gene Expression and Loss of Tooth/PDL/Alveolar Bone Tissue Contours in the Wnt1Cre/YAP/TAZ Mutant Mice Dentoalveolar Complex following Unloading
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pandya, M.; Gopinathan, G.; Tillberg, C.; Wang, J.; Luan, X.; Diekwisch, T.G.H. The Hippo Pathway Effectors YAP/TAZ Are Essential for Mineralized Tissue Homeostasis in the Alveolar Bone/Periodontal Complex. J. Dev. Biol. 2022, 10, 14. https://doi.org/10.3390/jdb10010014
Pandya M, Gopinathan G, Tillberg C, Wang J, Luan X, Diekwisch TGH. The Hippo Pathway Effectors YAP/TAZ Are Essential for Mineralized Tissue Homeostasis in the Alveolar Bone/Periodontal Complex. Journal of Developmental Biology. 2022; 10(1):14. https://doi.org/10.3390/jdb10010014
Chicago/Turabian StylePandya, Mirali, Gokul Gopinathan, Connie Tillberg, Jun Wang, Xianghong Luan, and Thomas G. H. Diekwisch. 2022. "The Hippo Pathway Effectors YAP/TAZ Are Essential for Mineralized Tissue Homeostasis in the Alveolar Bone/Periodontal Complex" Journal of Developmental Biology 10, no. 1: 14. https://doi.org/10.3390/jdb10010014