The Roles of the Wnt-Antagonists Axin and Lrp4 during Embryogenesis of the Red Flour Beetle Tribolium castaneum
Abstract
:1. Introduction
2. Materials and Methods
3. Results
3.1. The Wnt-Inhibitor Tc-axin Is Asymmetrically Expressed in the Egg and Dynamically with Distinct Patterns during Further Embryogenesis
3.2. Reduction of Tc-axin Function Results in Anterior Truncated, Mild Bicaudal and Symmetric Double Abdomen Embryos
3.3. Structure, Expression and Functional Analysis of Tc-lrp4
3.4. Pair-Rule and Segment-Polarity Gene Expression in Tc-lrp4 RNAi Embryos Reveal Instability of the Segment Boundary
4. Discussion
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Numbers (N =) | axinRNAi_20 | axinRNAi_50 | iB-04108-1 | iB-04108-2 | axinRNAi GFP-Strain | axin/Wnt8 Double RNAi | axin/Arrow Double RNAi |
---|---|---|---|---|---|---|---|
WT | 93 | 91 | 44 | - | 167 | 126 | 25 |
K-T-A | 15 | 8 | 4 | - | 7 | 19 | 1 |
T-A (−) | 6 | - | 6 | - | 4 | 9 | 10 |
A (−) | 7 | 5 | 15 | 1 | 9 | 12 | 34 |
part. cuticle | 12 | 4 | 24 | 6 | 9 | 80 | 39 |
T-A (+) | 5 | 7 | 8 | - | 3 | 5 | - |
A (+) | 5 | 3 | 18 | 3 | 10 | 15 | 12 |
A (+2 hg) | 9 | 6 | 11 | 11 | 6 | 62 | 21 |
cuticles with polarity reversal | 19 | 16 | 37 | 14 | 19 | 82 | 33 |
affected cuticles | 59 | 33 | 86 | 21 | 48 | 202 | 117 |
all cuticles | 152 | 124 | 130 | 21 | 215 | 328 | 142 |
empty eggs | 39 | 58 | 17 | 22 | 17 | 76 | 97 |
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Prühs, R.; Beermann, A.; Schröder, R. The Roles of the Wnt-Antagonists Axin and Lrp4 during Embryogenesis of the Red Flour Beetle Tribolium castaneum. J. Dev. Biol. 2017, 5, 10. https://doi.org/10.3390/jdb5040010
Prühs R, Beermann A, Schröder R. The Roles of the Wnt-Antagonists Axin and Lrp4 during Embryogenesis of the Red Flour Beetle Tribolium castaneum. Journal of Developmental Biology. 2017; 5(4):10. https://doi.org/10.3390/jdb5040010
Chicago/Turabian StylePrühs, Romy, Anke Beermann, and Reinhard Schröder. 2017. "The Roles of the Wnt-Antagonists Axin and Lrp4 during Embryogenesis of the Red Flour Beetle Tribolium castaneum" Journal of Developmental Biology 5, no. 4: 10. https://doi.org/10.3390/jdb5040010