The Tomato Feruloyl Transferase FHT Promoter Is an Accurate Identifier of Early Development and Stress-Induced Suberization
Abstract
:1. Introduction
2. Results
2.1. FHT Proteins of Different Plant Species Have a Conserved HxxxD Motif Involved in Catalysis and a DFGWG Motif Located at the C-Terminal End
2.2. Putative Cis Elements Found in Tomato FHT Promoter
2.3. Induction of ProSlFHT:GUS in Tomato Tissues Undergoing Developmental Suberization
2.4. Induction of ProSlFHT:GUS in Tomato Tissues Undergoing Wound Healing
2.5. Induction of ProSlFHT::GUS in Vascular Suberization Response in Tomato against Pathogens
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. DNA Constructs
4.3. Stable Transformation of Tomato
4.4. Detection of SlFHT Promoter Activity
4.5. Histological Methods
4.6. Wounding in Leaves
4.7. Bacterial Inoculation in Plants
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Group | Motif Name | Sequence | Motif | Function |
---|---|---|---|---|
Nº | ||||
Stress | ||||
WBOXNTCHN48 | CTGACY | 1 | “W box.” Elicitor-responsive element | |
WBOXNTERF3 | TGACY | 5 | “W box.” Response to wounding | |
OSE1ROOTNODULE | AAAGAT | 5 | Organ-specific elements (OSE) in infected cells of root nodules | |
OSE2ROOTNODULE | CTCTT | 2 | Organ-specific elements (OSE) in infected cells of root nodules | |
PREATPRODH | ACTCAT | 1 | Pro- or hypo-osmolarity-responsive element | |
RAV1AAT | CAACA | 5 | RAV1 binding site, cold responsiveness | |
MYB1AT | WAACCA | 3 | Element involved in dehydration responsiveness | |
MYBCORE | CNGTTR | 2 | Element involved in response to water stress | |
GT1GMSCAM4 | GAAAAA | 9 | Pathogenesis and salt-induced element | |
CURECORECR | GTAC | 10 | Copper-response element | |
CCAATBOX1 | CCAAT | 5 | HSE (heat shock element) | |
ANAERO1CONSENSUS | AAACAAA | 2 | Response to anaerobiosis | |
ACGTATERD1 | ACGT | 4 | Involved in etiolation-induced dehydration | |
Hormones | ||||
ABA | WBBOXPCWRKY1 | TTTGACY | 1 | WRKY proteins binding site, responsive to ABA |
MYB2CONSENSUSAT | YAACKG | 1 | MYB recognition site involved in dehydration and ABA response | |
MYCATERD1 | CATGTG | 1 | MYC binding site involved in response to dehydration and ABA | |
MYCATRD22 | CACATG | 1 | MYC binding site involved in response to dehydration and ABA | |
MYCCONSENSUSAT | CANNTG | 6 | MYC binding site involved in response to dehydration and ABA | |
SORLIP1AT | GCCAC | 4 | Light-inducible, root-specific, and ABA-responsive element | |
Salicylic acid | WBOXATNPR1 | TTGAC | 3 | “W-box”; binding site for SA-induced WRKY transcription factor |
Ethylene | ERELEE4 CGTCA-motif | AWTTCAAA | 1 | Ethylene-responsive element |
Auxin | CATATGGMSAUR | CATATG | 2 | Element involved in auxin responsiveness |
Gibberellin | GARE2OSREP1 | TAACGTA | 1 | Gibberellin-responsive element (GARE) |
GAREAT | TAACAAR | 2 | GARE (GA-responsive element) | |
MYBGAHV | TAACAAA | 2 | Central element of gibberellin (GA) response complex (GARC) | |
PYRIMIDINEBOXOSRAMY1A | CCTTTT | 2 | Gibberellin-response cis element of GARE and pyrimidine | |
TATCCACHVAL21 | TATCCAC | 1 | Conserved cis-acting response complex (GARC) | |
PYRIMIDINEBOXHVEPB1 | TTTTTTCC | 2 | “Pyrimidine box”. Required for GA induction | |
Organ-/Tissue-/Cell type-specific | ||||
ROOTMOTIFTAPOX1 | ATATT | 12 | Root specific element from rolD gene | |
SP8BFIBSP8BIB | TACTATT | 3 | Tuberous root specific element | |
RHERPATEXPA7 | KCACGW | 1 | Right part of RHEs (root-hair-specific cis-elements) | |
EBOXBNNAPA | CANNTG | 6 | RRE element. Tissue-specific activation of phenylpropanoids biosynthesis genes | |
RYREPEATBNNAPA | CATGCA | 1 | Required for seed-specific expression | |
POLLEN1LELAT52 | AGAAA | 12 | Element required for pollen-specific expression | |
CACTFTPPCA1 | YACT | 28 | Element related to mesophyll expression | |
BOXIINTPATPB | ATAGAA | 3 | Conserved element in promoters of plastids genes. | |
TAAAGSTKST1 | TAAAG | 12 | Dof1 protein controlling guard cell-specific gene expression | |
Others | ||||
MYBPZM | CCWACC | 3 | Core of consensus myb homolog binding site | |
MYBST1 | GGATA | 2 | Core motif of MybSt1 (a potato MYB homolog) binding site | |
RYREPEATLEGUMINBOX | CATGCAY | 1 | RY repeat found in seed-storage protein genes |
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Kashyap, A.; Jiménez-Jiménez, Á.; Figueras, M.; Serra, O.; Valls, M.; Coll, N.S. The Tomato Feruloyl Transferase FHT Promoter Is an Accurate Identifier of Early Development and Stress-Induced Suberization. Plants 2023, 12, 1890. https://doi.org/10.3390/plants12091890
Kashyap A, Jiménez-Jiménez Á, Figueras M, Serra O, Valls M, Coll NS. The Tomato Feruloyl Transferase FHT Promoter Is an Accurate Identifier of Early Development and Stress-Induced Suberization. Plants. 2023; 12(9):1890. https://doi.org/10.3390/plants12091890
Chicago/Turabian StyleKashyap, Anurag, Álvaro Jiménez-Jiménez, Mercè Figueras, Olga Serra, Marc Valls, and Nuria S. Coll. 2023. "The Tomato Feruloyl Transferase FHT Promoter Is an Accurate Identifier of Early Development and Stress-Induced Suberization" Plants 12, no. 9: 1890. https://doi.org/10.3390/plants12091890