Genetic Control of Effective Seedling Leaf Rust Resistance in Aegilops biuncialis Vis. Accessions from the VIR Collection
Abstract
:1. Introduction
2. Results
2.1. Parental Resistance
2.2. Genetic Control of Seedling Resistance to Pt
2.2.1. Hybridological Analysis
The Number of Genes for Pt Resistance
Allelic Relationship of Pt Resistance Genes in Goatgrass Accessions
2.2.2. Phytopathological Analysis
2.2.3. PCR Analysis
3. Discussion
4. Materials and Methods
4.1. Goatgrass Accessions
4.2. Pathogen Material
4.3. Hybridological Analysis
4.3.1. Crossing of the Accessions
4.3.2. Evaluation of Resistance
4.3.3. Statistical Analysis
4.4. Phytopathological Analysis
4.5. Analysis of Amplicons
- -
- Lr9—2 min at 92 °C; 35 cycles (1 min at 94 °C, 1 min at 4 °C, 1 min at 72 °C); final extension for 7 min at 72 °C [49];
- -
- Lr19—5 min at 94 °C; 40 cycles (30 s at 94 °C, 30 s at 60 °C, 1 min at 72 °C); final extension for 5 min at 72 °C [50];
- -
- Lr24—2 min at 95 °C; 35 cycles (1 min at 94 °C, 1 min at 60 °C, 1 min at 72 °C); final extension for 7 min at 72 °C [51];
- -
- Lr39—4 min at 94 °C; 30 cycles (30 s at 94 °C, 30 s at 55 °C, 30 s at 72 °C); final extension for 5 min at 72 °C [24];
- -
- Lr47—3 min at 94 °C; 7 cycles of touchdown (30 s at 94 °C for; in every cycle the annealing temperature steps down (70, 69, 68, 67, 66, 65, and 64 °C) for 30 s, 30 s at 72 °C); 35 cycles (30 s at 94 °C, 30 s at 63 °C, 30 s at 72 °C); final extension for 7 min at 72 °C [52].
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
- McIntosh, R.A.; Wellings, C.R.; Park, R.F. Wheat Rust—An Atlas of Resistance Genes; CSIRO Publications: Melbourne, Australia, 1995; pp. 1–200. [Google Scholar]
- Germán, S.; Barcellos, A.; Chaves, M.; Kohli, M.; Campos, P.; Viedma, L. The situation of common wheat rusts in the Southern Cone of America and perspectives for control. Austr. J. Agric. Res. 2007, 58, 620–630. [Google Scholar] [CrossRef]
- Prasad, P.; Savadi, S.; Bhardwaj, S.C.; Gupta, P.K. The progress of leaf rust research in wheat. Fungal Biol. 2020, 124, 537–550. [Google Scholar] [CrossRef]
- Huerta-Espino, J.; Singh, R.P.; Germán, S.; McCallum, B.D.; Park, R.F.; Chen, W.Q.; Bhardwaj, S.C.; Goyeau, H. Global status of wheat leaf rust caused by Puccinia triticina. Euphytica 2011, 179, 143–160. [Google Scholar] [CrossRef]
- Hassan, A.; Akram, M.U.; Hussain, M.A.; Bashir, M.A.; Mostafa, Y.S.; Alamri, S.A.M.; Hashem, M. Screening of different wheat genotypes against leaf rust and role of environmental factors affecting disease development. J. King Saud Univ. Sci. 2022, 34, 101991. [Google Scholar] [CrossRef]
- Kumar, K.; Jan, I.; Saripalli, G.; Sharma, P.K.; Mir, R.R.; Balyan, H.S.; Gupta, P.K. An update on resistance genes and their use in the development of leaf rust resistant cultivars in wheat. Front. Genet. 2022, 13, 816057. [Google Scholar] [CrossRef]
- Mathuria, R.C.; Singh, V.K.; Gogoi, R.; Aggarwal, R. Genetics of resistance to leaf rust pathogen in some Indian bread wheat cultivars. Indian Phytopath. 2016, 69, 197–202. [Google Scholar]
- Bolton, M.D.; Kolmer, J.A.; Garvin, D.F. Wheat leaf rust caused by Puccinia triticina. Mol. Plant Pathol. 2008, 9, 563–575. [Google Scholar] [CrossRef]
- Mapuranga, J.; Chang, J.; Zhao, J.; Liang, M.; Li, R.; Wu, Y.; Zhang, N.; Zhang, L.; Yang, W. The underexplored mechanisms of wheat resistance to leaf rust. Plants 2023, 12, 3996. [Google Scholar] [CrossRef] [PubMed]
- Tyryshkin, L.G.; Syukov, V.V.; Zaharov, V.G.; Zuev, E.V.; Gashimov, M.E.; Kolesova, M.A.; Chikida, N.N.; Ershova, M.A.; Belousova, M.H. Sources of effective resistance to fungal diseases in wheat and its relatives—Search, creation and use in breeding. Proc. Appl. Bot. Genet. Breed. 2012, 170, 187–201. (In Russian) [Google Scholar]
- Tyryshkin, L.G. Genetic Diversity of Wheat and Barley for Effective Diseases Resistance and the Possibility of Its Broadening. Doctoral Dissertation, N.I. Vavilov All-Russian Institute of Plant Industry, Saint Petersburg, Russia, 2007. (In Russian). [Google Scholar]
- Tyryshkin, L.G.; Lysenko, N.S.; Kolesova, M.A. Effective resistance to four fungal foliar diseases in samples of wild Triticum L. species from the VIR (N.I. Vavilov All-Russian Institute of Plant Genetic Resources) collection: View from Vavilov’s concepts of plant immunity. Plants 2022, 11, 3467. [Google Scholar] [CrossRef]
- El-Orabey, W.M. Virulence of some Puccinia triticina races to the effective wheat leaf rust resistant genes Lr9 and Lr19 under Egyptian field conditions. Physiol. Mol. Plant Pathol. 2018, 102, 163–172. [Google Scholar] [CrossRef]
- Bhardwaj, S.C.; Prashar, M.; Kumar, S.; Jain, S.K.; Datta, D. Lr19 resistance in wheat becomes susceptible to Puccinia triticina in India. Plant Dis. 2005, 89, 1360. [Google Scholar] [CrossRef]
- Boshoff, W.H.P.; Labuschagne, R.; Terefe, T.; Pretorius, Z.A.; Visser, B. New Puccinia triticina races on wheat in South Africa. Australas. Plant Pathol. 2018, 47, 325–334. [Google Scholar] [CrossRef]
- Figlan, S.; Ntushelo, K.; Mwadzingeni, L.; Terefe, T.; Tsilo, T.J.; Shimelis, H. Breeding wheat for durable leaf rust resistance in southern Africa: Variability, distribution, current control strategies, challenges, and future prospects. Front. Plant Sci. 2020, 11, 549. [Google Scholar] [CrossRef]
- Hanzalová, A.; Bartoš, P. The virulence spectrum of the wheat leaf rust population analyzed in the Czech Republic from 2002 to 2011. Czech J. Genet. Plant Breed. 2014, 50, 288–292. [Google Scholar] [CrossRef]
- Huerta-Espino, J.; Singh, R.P.; Reyna-Martinez, J. First detection of virulence to genes Lr9 and Lr25 conferring resistance to leaf rust of wheat caused by Puccinia triticina in Mexico. Plant Dis. 2008, 92, 311. [Google Scholar] [CrossRef]
- Huerta-Espino, J.; Singh, R.P. First report of virulence to wheat with leaf rust resistance gene Lr19 in Mexico. Plant Dis. 1994, 78, 640. [Google Scholar] [CrossRef]
- McCallum, B.D.; Seto-Goh, P.; Xue, A. Physiologic specialization of Puccinia triticina, the causal agent of wheat leaf rust, in Canada in 2008. Can. J. Plant. Pathol. 2011, 33, 541–549. [Google Scholar] [CrossRef]
- Navar, S.K.; Jain, S.K.; Prashar, M.; Bhardwaj, S.C.; Kumar, S.; Menon, M.K. Appearance of new pathotype of Puccinia recondita tritici virulent on Lr9 in India. Indian Phytopath. 2003, 56, 196–198. [Google Scholar]
- Park, R.F. Breeding cereals for rust resistance in Australia. Plant Pathol. 2008, 57, 591–602. [Google Scholar] [CrossRef]
- Roelfs, A.P.; Singh, R.P.; Saari, E.E. Rust Diseases of Wheat: Concepts and Methods of Disease Management; CIMMYT: Mexico City, Mexico, 1992; pp. 1–81. [Google Scholar]
- Singh, S.; Franks, C.D.; Huang, L.; Brown-Guedira, G.L.; Marshall, D.S.; Gill, B.S.; Fritz, A. Lr41, Lr39, and a leaf rust resistance gene from Aegilops cylindrica may be allelic and are located on wheat chromosome 2DS. Theor. Appl. Genet. 2004, 108, 586–591. [Google Scholar] [CrossRef] [PubMed]
- Talebi, R.; Mahboubi, M.; Naji, A.M.; Mehrabi, R. Physiological specialization of Puccinia triticina and genome-wide association mapping provide insights into the genetics of wheat leaf rust resistance in Iran. Sci. Rep. 2023, 13, 4398. [Google Scholar] [CrossRef] [PubMed]
- Yang, H.L.; Diao, W.D.; Yan, X.C.; Gebrewahid, T.-W.; Li, Z.F.; Yao, Z.J. Identification of genes for leaf rust resistance in seedlings of wheat cultivars from the Yellow-Huai Basin in China and slow rusting observations in field trials. Czech J. Genet. Plant Breed. 2023, 59, 219–234. [Google Scholar] [CrossRef]
- Agapova, V.D.; Vaganova, O.F.; Volkova, G.V. The efficiency of juvenile genes of orange leaf rust resistance of winter wheat during the germinal phase in the climate of the Russian south. Int. Res. J. 2020, 8, 163–167. (In Russian) [Google Scholar]
- Leonova, I.N.; Skolotneva, E.S.; Salina, E.A. Genome-wide association study of leaf rust resistance in Russian spring wheat varieties. BMC Plant Biol. 2020, 20, 135. [Google Scholar] [CrossRef] [PubMed]
- Markelova, T.S. Study of the structure and variability of the wheat brown rust population in the Volga region. AgroXXI 2007, 4–6, 37–40. (In Russian) [Google Scholar]
- Meshkova, L.V.; Rosseeva, L.P.; Korenyuk, E.A.; Belan, I.A. Dynamics of distribution of the wheat leaf rust pathotypes virulent to the cultivars with Lr9 gene in Omsk region. Mikol. Fitopatol. 2012, 46, 397–400. (In Russian) [Google Scholar]
- Plotnikova, L.Y.; Meshkova, L.V.; Gultyaeva, E.I.; Mitrofanova, O.P.; Lapochkina, I.F. A tendency towards leaf rust resistance decrease in common wheat introgression lines with genetic material from Aegilops speltoides Tausch. Vavilov J. Genet. Breed. 2018, 22, 560–567. (In Russian) [Google Scholar] [CrossRef]
- Sibikeev, S.N.; Konkova, E.A.; Salmova, M.F. Characteristic of the bread wheat leaf rust pathogen virulence in the Saratov region conditions. Agrar. Sci. J. 2020, 9, 40–44. (In Russian) [Google Scholar] [CrossRef]
- Skolotneva, E.S.; Leonova, I.N.; Bukatich, E.Y.; Boiko, N.I.; Piskarev, V.V.; Salina, E.A. Effectiveness of leaf rust resistance genes against Puccinia triticina populations in Western Siberia during 2008–2017. J. Plant Dis. Prot. 2018, 125, 549–555. [Google Scholar] [CrossRef]
- Adonina, I.G.; Timonova, E.M.; Salina, E.A. Introgressive hybridization of common wheat: Results and prospects. Rus. J. Genet. 2021, 57, 390–407. [Google Scholar] [CrossRef]
- Anikster, Y.; Manisterski, J.; Long, D.L.; Leonard, K.J. Resistance to leaf rust, stripe rust, and stem rust in Aegilops spp. in Israel. Plant Dis. 2005, 89, 303–308. [Google Scholar] [CrossRef] [PubMed]
- Cox, T.S.; Raupp, W.J.; Wilson, D.L.; Gill, B.S.; Leath, S.; Bockus, W.W.; Browder, L.E. Resistance to foliar diseases in a collection of Triticum tauschii germ plasm. Plant Dis. 1992, 76, 1061–1064. [Google Scholar] [CrossRef]
- Assefa, S.; Fehrmann, H. Resistance in Aegilops species against leaf rust, stem rust, Septoria tritici blotch, eyespot and powdery mildew of wheat. J. Plant Dis. Prot. 1998, 105, 624–631. [Google Scholar]
- Farkas, A.; Gaál, E.; Ivanizs, L.; Blavet, N.; Said, M.; Holušová, K.; Szőke-Pázsi, K.; Spitkó, T.; Mikó, P.; Türkösi, E.; et al. Chromosome genomics facilitates the marker development and selection of wheat-Aegilops biuncialis addition, substitution and translocation lines. Sci. Rep. 2023, 13, 20499. [Google Scholar] [CrossRef]
- Dimov, A.; Zaharieva, M.; Mihova, S. Rusts and powdery mildew resistance in Aegilops accessions from Bulgaria. In Biodiversity and Wheat Improvement; Damania, A.B., Ed.; John Wiley and Sons: New York, NY, USA, 1993; pp. 165–169. [Google Scholar]
- Tan, F.; Zhou, J.; Yang, Z.; Zhang, Y.; Pan, L.; Ren, Z. Characterization of a new synthetic wheat-Aegilops biuncialis partial amphiploid. J. Biotechnol. 2009, 8, 3215–3218. [Google Scholar]
- Goncharov, N.P.; Boguslavsky, R.L.; Orlova, E.A.; Belousova, M.K.; Aminov, N.K.; Konovalov, A.A.; Kondratenko, E.Y.; Gultyaeva, E.I. Leaf rust resistance in wheat amphidiploids. Let. Vavilov J. Genet. Breed. 2020, 6, 95–106. (In Russian) [Google Scholar]
- McIntosh, R.A.; Yamazaki, Y.; Dubcovsky, J.; Rogers, J.; Morris, C.; Appels, R.; Xia, X.C. Catalogue of Gene Symbols for Wheat. 2013. Available online: https://shigen.nig.ac.jp/wheat/komugi/genes/macgene/2013/GeneSymbol.pdf (accessed on 27 April 2024).
- McIntosh, R.A.; Dubcovsky, J.; Rogers, W.J.; Morris, C.; Appels, R.; Xia, X.C. Catalogue of Gene Symbols for Wheat: 2015–2016 Supplement. 2015. Available online: https://shigen.nig.ac.jp/wheat/komugi/genes/macgene/supplement2015.pdf (accessed on 27 April 2024).
- Marais, F.; Marais, A.; McCallum, B.; Pretorius, Z. Transfer of leaf rust and stripe rust resistance genes Lr62 and Yr42 from Aegilops neglecta Req. ex Bertol. to common wheat. Crop Sci. 2009, 49, 871–879. [Google Scholar] [CrossRef]
- Poudel, R.S. The Acquisition of Useful Disease Resistance Genes for Hard Red Winter Wheat Improvement. Master’ Thesis, North Dakota State University, Fargo, ND, USA, 2015. [Google Scholar]
- Kuraparthy, V.; Sood, S.; Chhuneja, P.; Dhaliwal, H.S.; Kaur, S.; Bowden, R.L.; Gill, B.S. A cryptic wheat–Aegilops triuncialis translocation with leaf rust resistance gene Lr58. Crop Sci. 2007, 47, 1995–2003. [Google Scholar] [CrossRef]
- Kolesova, M.A. Characterization of Aegilops biuncialis Viz. samples for juvenile resistance to fungal diseases. Mod. Sci. Success 2017, 2, 126–129. (In Russian) [Google Scholar]
- Kolesova, M.A.; Lysenko, N.S.; Tyryshkin, L.G. Resistance to diseases in samples of rare wheat species from the N.I. Vavilov All-Russian Institute of Plant Genetic Resources. Cereal Res. Comm. 2022, 50, 287–296. [Google Scholar] [CrossRef]
- Gupta, S.K.; Charpe, A.; Koul, S.; Prabhu, K.V.; Haq, Q.M.R. Development and validation of molecular markers linked to an Aegilops umbellulata-derived leaf-rust-resistance gene, Lr9, for marker-assisted selection in bread wheat. Genome 2005, 48, 823–830. [Google Scholar] [CrossRef] [PubMed]
- Prins, R.; Groenewald, J.Z.; Marais, G.F.; Snape, J.W.; Koebner, R.M.D. AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat. Theor. Appl. Genet. 2001, 103, 618–624. [Google Scholar] [CrossRef]
- Gupta, S.K.; Charpe, A.; Koul, S.; Haque, Q.M.R.; Prabhu, K.V. Development and validation of SCAR markers co-segregating with an Agropyron elongatum derived leaf rust resistance gene Lr24 in wheat. Euphytica 2006, 150, 233–240. [Google Scholar] [CrossRef]
- Helguera, M.; Khan, I.A.; Dubcovsky, J. Development of PCR markers for the wheat leaf rust resistance gene Lr47. Theor. Appl. Genet. 2000, 100, 1137–1143. [Google Scholar] [CrossRef]
- Keed, B.R.; White, N.H. Quantitative effects of leaf and stem rust on yield and quality of wheat. Aust. J. Exp. Agric. 1971, 11, 550–555. [Google Scholar] [CrossRef]
- Schneider, A.; Molnár, I.; Molnár-Láng, M. Utilization of Aegilops (goatgrass) species to widen the genetic diversity of cultivated wheat. Euphytica 2008, 163, 1–19. [Google Scholar] [CrossRef]
- Kolesova, M.A.; Tyryshkin, L.G. Characterization of samples of Aegilops L. species by effective resistance to diseases. Achiev. Sci. Technol. Agribus. 2015, 29, 20–23. (In Russian) [Google Scholar]
- Tyryshkin, L.G.; Kolesova, M.A. The use of molecular-genetic and phytopathological methods to identify genes for effective leaf rust resistance in Aegilops accessions. Proc. Appl. Bot. Genet. Breed. 2020, 181, 87–95. (In Russian) [Google Scholar] [CrossRef]
- Khan, M.K.; Pandey, A.; Hamurcu, M.; Avsaroglu, Z.Z.; Ozbek, M.; Omay, A.H.; Elbasan, F.; Omay, M.R.; Gokmen, F.; Topal, A.; et al. Variability in physiological traits reveals boron toxicity tolerance in Aegilops species. Front. Plant Sci. 2021, 12, 736614. [Google Scholar] [CrossRef]
- Khan, M.K.; Pandey, A.; Hamurcu, M.; Germ, M.; Yilmaz, F.G.; Ozbek, M.; Avsaroglu, Z.Z.; Topal, A.; Gezgin, S. Nutrient homeostasis of Aegilops accessions differing in B tolerance level under boron toxic growth conditions. Biology 2022, 11, 1094. [Google Scholar] [CrossRef] [PubMed]
- Darko, E.; Khalil, R.; Dobi, Z.; Kovács, V.; Szalai, G.; Janda, T.; Molnár, I. Addition of Aegilops biuncialis chromosomes 2M or 3M improves the salt tolerance of wheat in different way. Sci. Rep. 2020, 10, 22327. [Google Scholar] [CrossRef] [PubMed]
- Molnár, I.; Gáspár, L.; Sárvári, É.; Dulai, S.; Hoffmann, B.; Molnár-Láng, M.; Galiba, G. Physiological and morphological responses to water stress in Aegilops biuncialis and Triticum aestivum genotypes with differing tolerance to drought. Funct. Plant Biol. 2004, 31, 1149–1159. [Google Scholar] [CrossRef] [PubMed]
- Olivera, P.D.; Rouse, M.N.; Jin, Y. Identification of new sources of resistance to wheat stem rust in Aegilops spp. in the tertiary genepool of wheat. Front. Plant Sci. 2018, 9, 1719. [Google Scholar] [CrossRef] [PubMed]
- Tyryshkin, L.G. The Study of Genetic Control of Resistance to Diseases in Self-Pollinating Crops: Methods; VIR: St. Petersburg, Russia, 2010; pp. 1–35. (In Russian) [Google Scholar]
- Abou-Elseoud, M.S.; Kamara, A.M.; Alaa-Eldein, O.A.; El-Bebany, A.F.; Ashmawy, N.A.; Draz, I.S. Identification of leaf rust resistance genes in Egyptian wheat cultivars by multipathotypes and molecular markers. J. Plant Sci. 2014, 2, 145–151. [Google Scholar] [CrossRef]
- Gultyaeva, E.I.; Shaydayuk, E.L.; Veselova, V.V.; Smirnova, R.E.; Zuev, E.V.; Khakimova, A.G.; Mitrofanova, O.P. Diversity of new Russian bread wheat cultivars according to leaf rust resistance genes. Proc. Appl. Bot. Genet. Breed. 2022, 183, 208–218. (In Russian) [Google Scholar] [CrossRef]
- Kokhmetova, A.; Rsaliyev, S.; Atishova, M.; Kumarbayeva, M.; Malysheva, A.; Keishilov, Z.; Zhanuzak, D.; Bolatbekova, A. Evaluation of wheat germplasm for resistance to leaf rust (Puccinia triticina) and identification of the sources of Lr resistance genes using molecular markers. Plants 2021, 10, 1484. [Google Scholar] [CrossRef] [PubMed]
- Liu, X.J.; Liu, X.C.; Sun, H.Y.; Hao, C.Y.; Wang, X.X.; Rong, Z.J.; Feng, Z.Y. Validation of CAPS marker WR003 for the leaf rust resistance gene Lr1 and the molecular evolution of Lr1 in wheat. Czech J. Genet. Plant Breed. 2022, 58, 223–232. [Google Scholar] [CrossRef]
- Dzhenin, S.V.; Lapochkina, I.F.; Zhemchuzhina, A.I.; Kovalenko, E.D. Donors of spring wheat resistant to the leaf rust with the genetic material of Aegilops speltoides L., Aegilops triuncialis L., Triticum kiharae Dorof. et Migusnh. Russ. Agric. Sci. 2009, 5, 3–7. (In Russian) [Google Scholar]
- Gajnullin, N.R.; Lapochkina, I.F.; Zhemchuzhina, A.I.; Kiseleva, M.I.; Kolomiets, T.M.; Kovalenko, E.D. Phytopathological and molecular genetic identification of leaf rust resistance genes in common wheat accessions with alien genetic material. Russ. J. Genet. 2007, 43, 875–881. [Google Scholar] [CrossRef]
- Koláriková, L.; Svobodová-Leišová, L.; Hanzalová, A.; Holubec, V.; Jungová, M.; Esimbekova, M. Leaf rust resistance genes in Aegilops genus: Occurrence and efficiency. Eur. J. Plant. Pathol. 2023, 167, 335–348. [Google Scholar] [CrossRef]
- Tyryshkin, L.G. About DNA-markers as sole criteria for postulation of Lr-genes of the Triticum aestivum L. resistance to Puccinia triticina Erikss: Critical essay. Agric. Biol. 2010, 45, 76–81. (In Russian) [Google Scholar]
- Kolesova, M.A.; Chikida, N.N.; Belousova, M.K.; Tyryshkin, L.G. Effective resistance to powdery mildew in Aegilops L. accessions. Proc. Appl. Bot. Genet. Breed. 2020, 181, 135–140. (In Russian) [Google Scholar] [CrossRef]
- Data Platform for Plant Genetic Resources. Available online: https://db.vir.nw.ru/virdb/maindb?lang=en (accessed on 20 July 2024).
- Tyryshkin, L.G. Modification Variability of Virulence and Aggressiveness in Phytopathogens of Cereal Crops: Conclusions, Consequences, Possibilities of Practical Application; Saint Petersburg State Agrarian University: Saint Petersburg, Russia, 2016; pp. 1–137. (In Russian) [Google Scholar]
- Merezhko, A.F.; Erokhin, L.M.; Yudin, A.E. The Effective Method of Cereals Pollination: Methods; VIR: Saint Petersburg, Russia, 1973; pp. 1–11. (In Russian) [Google Scholar]
- Mains, E.B.; Jackson, H.S. Physiological specialization in leaf rust of wheat, Puccinia triticina Erikss. Phytopathology 1926, 16, 89–120. [Google Scholar]
- Dospekhov, B.A. Methods of Field Experiment, 5th ed.; Agropromizdat: Moscow, Russia, 1985; pp. 1–351. (In Russian) [Google Scholar]
- Edwards, K.; Johnstone, C.; Thompson, C. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucl. Acids Res. 1991, 19, 1349. [Google Scholar] [CrossRef]
- Dorokhov, D.B.; Klocke, E. A rapid and economic technique for RAPD analysis of plant genomes. Russ. J. Genet. 1997, 33, 358–365. [Google Scholar]
VIR Catalogue №, k- | Infection Type after Inoculation with | Amplified Fragment Presence | |
---|---|---|---|
Complex Populations | Clones Virulent to Lr9 | ||
1145 | 0 | 0 | − |
2892 | 0 | 0 | − |
2900 | 0 | 0 | + |
2452 | 0 | 0 | − |
2531 | 0 | 0 | − |
3006 | 0 | 0 | − |
4157 | 0 | 0 | − |
4195 | 0 | 0 | − |
3003 | 3 | 3 | + |
‘Thatcher’ NIL Lr9 | 0 | 3 | + |
Hybrid Combination | Ratio of Phenotypes R:S * | χ2 | p | |
---|---|---|---|---|
Observed | Expected | |||
k-1145 × k-3003 | 73:28 | 3:1 (1 dominant gene) | 0.40 | 0.53 |
49:15 (1 dominant gene + 2 complementary recessive genes) | 1.03 | 0.31 | ||
43:21 (1 recessive gene + 2 complementary dominant genes) | 1.19 | 0.28 | ||
51:13 (1 dominant + 2 complementary: recessive and dominant) | 3.43 | 0.06 | ||
k-2892 × k-3003 | 79:33 | 3:1 | 1.19 | 0.31 |
49:15 | 2.27 | 0.13 | ||
43:21 | 0.57 | 0.45 | ||
k-2900 × k-3003 | 78:32 | 3:1 | 0.98 | 0.32 |
49:15 | 1.96 | 0.16 | ||
43:21 | 0.69 | 0.41 | ||
k-2452 × k-3003 | 75:27 | 3:1 | 0.12 | 0.73 |
49:15 | 0.52 | 0.47 | ||
43:21 | 1.86 | 0.17 | ||
51:13 | 2.39 | 0.12 | ||
k-2531 × k-3003 | 70: 25 | 3:1 | 0.09 | 0.76 |
49:15 | 0.44 | 0.51 | ||
43:21 | 1.82 | 0.18 | ||
51:13 | 2.12 | 0.15 | ||
13:3 (1 dominant gene + 1 recessive gene) | 3.57 | 0.06 | ||
k-3006 × k-3003 | 69:26 | 3:1 | 0.28 | 0.60 |
49:15 | 0.82 | 0.37 | ||
43:21 | 1.28 | 0.26 | ||
51:13 | 2.92 | 0.09 | ||
k-4157 × k-3003 | 72:28 | 3:1 | 0.48 | 0.49 |
49:15 | 1.16 | 0.28 | ||
43:21 | 1.05 | 0.31 | ||
51:13 | 3.65 | 0.06 | ||
k-4195 × k-3003 | 72:25 | 3:1 | 0.03 | 0.86 |
49:15 | 0.29 | 0.59 | ||
43:21 | 2.18 | 0.14 | ||
51:13 | 1.79 | 0.18 | ||
13:3 | 3.14 | 0.08 |
Hybrid Combination | Ratio of Families R:RS:S * | χ2 | p | |
---|---|---|---|---|
Observed | Expected | |||
k-1145 × k-3003 | 14:29:15 | 1:2:1 (1 gene) | 0.03 | 0.99 |
19:38:7 (1 gene + 2 complementary genes) | 13.27 | 0.001 | ||
k 2892 × k-3003 | 20:43:18 | 1:2:1 | 0.41 | 0.81 |
19:38:7 | 10.65 | 0.004 | ||
k-2900 × k-3003 | 21:48:24 | 1:2:1 | 0.29 | 0.87 |
19: 38:7 | 21.32 | 0 | ||
k-2452 × k-3003 | 13:30:16 | 1:2:1 | 0.32 | 0.85 |
19:38:7 | 16.01 | 0 | ||
k-2531 × k-3003 | 21: 45:26 | 1:2:1 | 0.59 | 0.75 |
19:38:7 | 28.40 | 0 | ||
7:8:1 (2 genes) | 80.54 | 0 | ||
k-3006 × k-3003 | 16:35:18 | 1:2:1 | 0.13 | 0.94 |
19:38:7 | 16.33 | 0 | ||
k-4157 × k-3003 | 27:72:24 | 1:2:1 | 3.73 | 0.16 |
19:38:7 | 10.76 | 0.005 | ||
k-4195 × k-3003 | 26:50:20 | 1:2:1 | 0.92 | 0.63 |
19:38:7 | 9.67 | 0.007 | ||
7:8:1 | 38.85 | 0 |
Parents | ♀ | ||||||
---|---|---|---|---|---|---|---|
♂ | k-2892 | k-2900 | k-2452 | k-2531 | k-3006 | k-4157 | k-4195 |
k-1145 | 92:0 | 105:0 | 95:0 | 103:0 | 96:0 | 97:0 | 102:0 |
k-2892 | - | 99:0 | 98:0 | 98:0 | 107:0 | 95:0 | 108:0 |
k-2900 | - | - | 107:0 | 100:0 | 105:0 | 99:0 | 107:0 |
k-2452 | - | - | - | 97:0 | 108:0 | 94:0 | 106:0 |
k-2531 | - | - | - | - | 102:0 | 102:0 | 100:0 |
k-3006 | - | - | - | - | - | 100:0 | 102:0 |
k-4157 | - | - | - | - | - | - | 107:0 |
Catalog No. kk- | Origin | Collecting Site | Collecting Year | Longitude | Latitude |
---|---|---|---|---|---|
1145 | USSR | Nagorno-Karabakh, Gadrut district, Banazur village, surroundings | 1971 | E 47 | N 39 |
2452 | Greece | Attica, Athens, surroundings | 1986 | E 23 | N 37 |
2531 | USSR | Crimea, Ai-Donil village, surroundings, Yalta district | 1983 | E 34 | N 44 |
2892 | Bulgaria | Varna region, Albena-Batovo road, hills | 1990 | E 27 | N 43 |
2900 | Bulgaria | Burgas region, Yablochevo village, Dolgopol-Aitus road | 1990 | E 27 | N 42 |
3003 | USSR | Crimea, Bulganak Hill Field | 1988 | E 36 | N 45 |
3006 | USSR | Crimea, Arabat Bay coast, quarries | 1988 | E 35 | N 45 |
4157 | Greece | Thessaly | 2007 | E 21 | N 39 |
4195 | Greece | Unknown | 2007 | - | - |
Gene | Chromosome Localization | PCR Marker | Primer Sequence (5′-3′) | Fragment Size (bp) |
---|---|---|---|---|
Lr9 | 6B | SCS5550 | TGC GCC CTT CAA AGG AAG TGC GCC CTT CTG AAC TGT AT | 550 |
Lr19 | 7D | Gb | CAT CCT TGG GGA CCT C CCA GCT CGC ATA CAT CCA | 130 |
Lr24 | 3D | SCS1302607 | CGC AGG TTC CAA ATA CTT TTC CGC AGG TTC TAC CTA ATG CAA | 607 |
Lr39 | 1D | GDM35 | CCT GCT CTG CCC TAG ATA CG ATG TGA ATG TGA TGC ATG CA | 190 |
Lr47 | 7A | PS10 | GCT GAT GAC CCT GAC CGG T TCT TCA TGC CCG GTC GGG T | 282 |
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Kolesova, M.A.; Tyryshkin, L.G. Genetic Control of Effective Seedling Leaf Rust Resistance in Aegilops biuncialis Vis. Accessions from the VIR Collection. Plants 2024, 13, 2199. https://doi.org/10.3390/plants13162199
Kolesova MA, Tyryshkin LG. Genetic Control of Effective Seedling Leaf Rust Resistance in Aegilops biuncialis Vis. Accessions from the VIR Collection. Plants. 2024; 13(16):2199. https://doi.org/10.3390/plants13162199
Chicago/Turabian StyleKolesova, Maria A., and Lev G. Tyryshkin. 2024. "Genetic Control of Effective Seedling Leaf Rust Resistance in Aegilops biuncialis Vis. Accessions from the VIR Collection" Plants 13, no. 16: 2199. https://doi.org/10.3390/plants13162199