A Fast, Efficient, and Tissue-Culture-Independent Genetic Transformation Method for Panax notoginseng and Lilium regale
Abstract
:1. Introduction
2. Results
2.1. Establishment of Agrobacterium-Based Injection Rhizome Transformation Method in P. notoginseng
2.2. Agrobacterium-Based Injection in the Bulb Meristems Is a Fast and Efficient L. regale Genetic Transformation Method
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. A. tumefaciens Strain, Binary Vector, and Culture Preparation
4.3. Plant Preparation and Infection
4.4. PCR Screening of Positive Transgenic P. notoginseng and L. regale Plants
4.5. qRT-PCR-Based Detection of DsRed2 Expression
4.6. Analysis of Red Fluorescent Protein Expression through Western Blot
4.7. Observation of Specific Red Fluorescence in Different Organs of DsRed2 Transgenic Plants Using Laser Confocal Microscopy
4.8. Observing Red Fluorescence in Transgenic Plants Using a Handheld Fluorescence Detector
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Deng, J.; Li, W.; Li, X.; Liu, D.; Liu, G. A Fast, Efficient, and Tissue-Culture-Independent Genetic Transformation Method for Panax notoginseng and Lilium regale. Plants 2024, 13, 2509. https://doi.org/10.3390/plants13172509
Deng J, Li W, Li X, Liu D, Liu G. A Fast, Efficient, and Tissue-Culture-Independent Genetic Transformation Method for Panax notoginseng and Lilium regale. Plants. 2024; 13(17):2509. https://doi.org/10.3390/plants13172509
Chicago/Turabian StyleDeng, Jie, Wenyun Li, Xiaomin Li, Diqiu Liu, and Guanze Liu. 2024. "A Fast, Efficient, and Tissue-Culture-Independent Genetic Transformation Method for Panax notoginseng and Lilium regale" Plants 13, no. 17: 2509. https://doi.org/10.3390/plants13172509