Evaluation of the Antiwrinkle Activity of Enriched Isatidis Folium Extract and an HPLC–UV Method for the Quality Control of Its Cream Products
Abstract
:1. Introduction
2. Results and Discussion
2.1. Antiwrinkle Activity
2.2. Structural Characterization of the Main Compounds from Enriched Isatidis Folium Extract
2.3. Optimizing the Analytical Method
2.4. Method Validation
2.4.1. Linearity, LOD, and LOQ
2.4.2. Accuracy and Precision
2.4.3. Repeatability
2.4.4. Recoveries
2.5. Optimization of Sample Preparation for Extracting TMCA in Developed Antiwrinkle Cream Products
2.6. Method Application to Raw Materials and Products
3. Materials and Methods
3.1. Plant Material and Reagents
3.2. Preparing Enriched Isatidis Folium Extract and Cosmetic Creams
3.3. Preparation of Sample and Standard Solution
3.4. Optimizing Experimental Parameters
3.5. HPLC-PDA-ESI-MS/MS Analysis
3.6. Quality Control of Enriched Isatidis Folium Extract and Developed Antiwrinkle Cream Products
3.7. Cell Culture
3.8. Real-Time Reverse-Transcription Polymerase Chain Reaction (Real-Time RT-PCR)
3.9. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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No. | Rt a (min) | UV λ (nm) | Precursorion (m/z) | Production (m/z) | Molecular Formula | Identification |
---|---|---|---|---|---|---|
1 | 40.19 | 213, 269, 336 | 431.05 (M-H)− | 311.12 (M-H-C4H9O4)− | C21H20O10 | isovitexin |
2 | 40.82 | 213, 269, 338 | 592.65 (M-H)− | 311.12 (M-Glcb-C4H9O4-H)− 431.00 (M-Glc-H)− | C27H30O15 | isovitexin-3″-O-glucopyranoside |
3 | 41.78 | 209, 270, 346 | 461.20 (M-H)− | 341.28 (M-H-C4H9O4)− | C22H22O11 | isoscoparin |
4 | 42.35 | 208, 270, 348 | 624.03 (M-H)− | 341.28 (M-Glc-C4H9O4-H)− 461.12 (M-Glc–H)− | C28H32O16 | isoscoparin-3″-O-glucopyranoside |
5 | 62.43 | 229,302 | 239.00 (M-H)+ | 239.00 (M + H)+ | C12H14O5 | TMCAc |
Run | A: Sonication Time (min) | B: Methanol-to-Material Ratio (mL/g) | Content of TMCA Y (μg/g) |
---|---|---|---|
1 | 40 (0) | 7 (0) | 30.2 |
2 | 40 (0) | 4 (−1) | 27.3 |
3 | 40 (0) | 7 (0) | 29.7 |
4 | 40 (0) | 7 (0) | 29.6 |
5 | 40 (0) | 10 (1) | 25.2 |
6 | 50 (+1) | 10 (+1) | 21.3 |
7 | 40 (0) | 7 (0) | 29.4 |
8 | 50 (+1) | 4 (−1) | 20.8 |
9 | 40 (0) | 7 (0) | 29.7 |
10 | 30 (−1) | 10 (+1) | 21.9 |
11 | 50 (1) | 7(0) | 24.2 |
12 | 30 (−1) | 4 (−1) | 25.8 |
13 | 30 (−1) | 7 (0) | 26.0 |
Sample | Batch Number | Concentration |
---|---|---|
The powder of enriched Isatidis Folium extract | B-1 | 126.32 ± 0.32 mg/g |
B-2 | 127.45 ± 0.21 mg/g | |
B-3 | 126.61 ± 0.01 mg/g | |
Anti-wrinkle cream products | C-1 | 29.2 ± 0.01 μg/g |
C-2 | 28.9 ± 0.02 μg/g | |
C-3 | 29.6 ± 0.01 μg/g |
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Gao, D.; Cho, C.W.; Kim, C.T.; Jeong, W.S.; Kang, J.S. Evaluation of the Antiwrinkle Activity of Enriched Isatidis Folium Extract and an HPLC–UV Method for the Quality Control of Its Cream Products. Plants 2020, 9, 1586. https://doi.org/10.3390/plants9111586
Gao D, Cho CW, Kim CT, Jeong WS, Kang JS. Evaluation of the Antiwrinkle Activity of Enriched Isatidis Folium Extract and an HPLC–UV Method for the Quality Control of Its Cream Products. Plants. 2020; 9(11):1586. https://doi.org/10.3390/plants9111586
Chicago/Turabian StyleGao, Dan, Chong Woon Cho, Cheong Taek Kim, Won Seok Jeong, and Jong Seong Kang. 2020. "Evaluation of the Antiwrinkle Activity of Enriched Isatidis Folium Extract and an HPLC–UV Method for the Quality Control of Its Cream Products" Plants 9, no. 11: 1586. https://doi.org/10.3390/plants9111586