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Volume 11
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10.3390/proteomes11020015
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Article

Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer

by
Abraham Josué Nevárez-Ramírez
1,2,
Ana Laura Guzmán-Ortiz
1,
Pedro Cortes-Reynosa
3,
Eduardo Perez-Salazar
3,
Gustavo Alberto Jaimes-Ortega
1,4,
Ricardo Valle-Rios
1,4,
Álvaro Marín-Hernández
5,
José S. Rodríguez-Zavala
5,
Eliel Ruiz-May
6,
José Luis Castrejón-Flores
2 and
Héctor Quezada
1,*
1
Laboratorio de Investigación en Inmunología y Proteómica, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, Doctores, Mexico City 06720, Mexico
2
Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Av. Acueducto s/n, Barrio La Laguna, Mexico City 07340, Mexico
3
Departamento de Biología Celular, CINVESTAV-IPN, Av Instituto Politécnico Nacional 2508, San Pedro Zacatenco, Mexico City 07360, Mexico
4
División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Circuito interior, Av. Universidad 3000, Ciudad Universitaria, Coyoacán, Mexico City 04510, Mexico
5
Departamento de Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Belisario Domínguez—Sección XVI, Mexico City 14080, Mexico
6
Red de Estudios Moleculares Avanzados, Clúster Científico y Tecnológico BioMimic®, Instituto de Ecología A.C. (INECOL), Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Mexico
*
Author to whom correspondence should be addressed.
Proteomes 2023, 11(2), 15; https://doi.org/10.3390/proteomes11020015
Submission received: 27 January 2023 / Revised: 25 March 2023 / Accepted: 3 April 2023 / Published: 5 April 2023
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Abstract

Cellular interactions within the bone marrow microenvironment modulate the properties of subsets of leukemic cells leading to the development of drug-resistant phenotypes. The intercellular transfer of proteins and organelles contributes to this process but the set of transferred proteins and their effects in the receiving cells remain unclear. This study aimed to detect the intercellular protein transfer from mouse bone marrow stromal cells (OP9 cell line) to human T-lymphoblasts (CCRF-CEM cell line) using nanoLC-MS/MS-based shotgun proteomics in a 3D co-culture system. After 24 h of co-culture, 1513 and 67 proteins from human and mouse origin, respectively, were identified in CCRF-CEM cells. The presence of mouse proteins in the human cell line, detected by analyzing the differences in amino acid sequences of orthologous peptides, was interpreted as the result of intercellular transfer. The transferred proteins might have contributed to the observed resistance to vincristine, methotrexate, and hydrogen peroxide in the co-cultured leukemic cells. Our results suggest that shotgun proteomic analyses of co-cultured cells from different species could be a simple option to get a preliminary survey of the proteins exchanged among interacting cells.
Keywords: leukemia; tumor microenvironment; TSPO; spheroids; proteomics leukemia; tumor microenvironment; TSPO; spheroids; proteomics
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MDPI and ACS Style

Nevárez-Ramírez, A.J.; Guzmán-Ortiz, A.L.; Cortes-Reynosa, P.; Perez-Salazar, E.; Jaimes-Ortega, G.A.; Valle-Rios, R.; Marín-Hernández, Á.; Rodríguez-Zavala, J.S.; Ruiz-May, E.; Castrejón-Flores, J.L.; et al. Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer. Proteomes 2023, 11, 15. https://doi.org/10.3390/proteomes11020015

AMA Style

Nevárez-Ramírez AJ, Guzmán-Ortiz AL, Cortes-Reynosa P, Perez-Salazar E, Jaimes-Ortega GA, Valle-Rios R, Marín-Hernández Á, Rodríguez-Zavala JS, Ruiz-May E, Castrejón-Flores JL, et al. Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer. Proteomes. 2023; 11(2):15. https://doi.org/10.3390/proteomes11020015

Chicago/Turabian Style

Nevárez-Ramírez, Abraham Josué, Ana Laura Guzmán-Ortiz, Pedro Cortes-Reynosa, Eduardo Perez-Salazar, Gustavo Alberto Jaimes-Ortega, Ricardo Valle-Rios, Álvaro Marín-Hernández, José S. Rodríguez-Zavala, Eliel Ruiz-May, José Luis Castrejón-Flores, and et al. 2023. "Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer" Proteomes 11, no. 2: 15. https://doi.org/10.3390/proteomes11020015

APA Style

Nevárez-Ramírez, A. J., Guzmán-Ortiz, A. L., Cortes-Reynosa, P., Perez-Salazar, E., Jaimes-Ortega, G. A., Valle-Rios, R., Marín-Hernández, Á., Rodríguez-Zavala, J. S., Ruiz-May, E., Castrejón-Flores, J. L., & Quezada, H. (2023). Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer. Proteomes, 11(2), 15. https://doi.org/10.3390/proteomes11020015

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