Simultaneous Detection of Serotonin and 17β-Estradiol Using rGO/SPCE Modified with Cu(II) Complex: A Novel Approach for PMDD Diagnosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Apparatus
2.3. Fabrication of Cu(LNO2)2/rGO/SPCE
2.4. Electrochemical Measurements
3. Results
3.1. Morphological Characterization of Cu(LNO2)2/rGO/SPCE
3.2. Electrochemical Characterization of Cu(LNO2)2/rGO/SPCE
3.3. Electrochemical Performance of Cu(LNO2)2/rGO/SPCE
3.4. Optimization of Parameters for LSV Serotonin and 17β-Estradiol Simultaneous Detection
3.5. Analytical Performance of Cu(LNO2)2/rGO/SPCE
3.6. Repeatability, Stability, and Selectivity of the Cu(LNO2)2/rGO/SPCE
3.7. Evaluation of the Performance of Cu(LNO2)2/rGO/SPCE for Detection Serotonin and 17β-Estradiol
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Analyte | Electrode | Lineal Range µmol L−1 | LOD nmol L−1 | Sample | Ref. |
---|---|---|---|---|---|
Serotonin | FeC-AuNPs-MWCNT/SPCE | 0.05–20 | 17 | urine | [46] |
DGNW/SPGE | 1–500 | 280 | urine | [47] | |
TiO2NP/AuNP/PNBDES/SPCE | 0.3–20 | 2.47 | human blood serum | [28] | |
CDP-Choline/MCPE | 10–30 | 3960 | No reported | [48] | |
ZrO2–CuO–CeO2/Gr/CPE | 7.9–205 | 3.49 | human urine/plasma samples | [29] | |
ITO/CuO-Cu2O | 2–100 | 120 | human blood serum | [49] | |
p-AZ/MWCNT-GR/CFMEA | 0.1–20 | 14.2 | human serum | [50] | |
Cu(LNO2)2/rGO/SPCE | 2–42 | 42 | human serum/Urine | This Work | |
17β-estradiol | α-Fe2O3-CNT/GCE | 0.005–0.1 | 4.4 | pharmaceutical/lake water/synthetic urine | [51] |
rGO-AuNPs/CNT/SPE | 0.05–1.00 | 3 | drinking water | [30] | |
NiFe2O4-MC/GCE | 0.02–0.566 | 6.88 | estradiol in tablet | [52] | |
wMC/GCE | 0.05–10.0 | 8.3 | Milk/real water | [31] | |
CDs-PANI/GCE | 0.001–100 | 43 | human serum/different types of water | [35] | |
CeO2 NPs/CPE | 0.01–0.1 | 1.3/4.3 | underground and lake water | [53] | |
MIP-MWCNT/GCE | 0.05–5.00 | 10 | natural water samples | [54] | |
Cu(LNO2)2/rGO/SPCE | 2–42 | 53 | human serum/Urine | This work |
Sample | Analyte | Added, mmol L−1 | Found mmol L−1 | Recovery, % | RSD, % |
---|---|---|---|---|---|
Human Serum * | Serotonin | 0.15 | 0.134 | 89.3 | 2.8 |
17β-estradiol | 0.15 | 0.121 | 85.1 | 6.9 | |
Human Urine † | Serotonin | 0.15 | 0.145 | 96.7 | 1.9 |
17β-estradiol | 0.15 | 0.144 | 96.0 | 4.3 |
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Núñez, C.; Nelson, R.; Tabilo, G.; Pefaur, P.; Castillo, R.; Mestra, A. Simultaneous Detection of Serotonin and 17β-Estradiol Using rGO/SPCE Modified with Cu(II) Complex: A Novel Approach for PMDD Diagnosis. Chemosensors 2024, 12, 164. https://doi.org/10.3390/chemosensors12080164
Núñez C, Nelson R, Tabilo G, Pefaur P, Castillo R, Mestra A. Simultaneous Detection of Serotonin and 17β-Estradiol Using rGO/SPCE Modified with Cu(II) Complex: A Novel Approach for PMDD Diagnosis. Chemosensors. 2024; 12(8):164. https://doi.org/10.3390/chemosensors12080164
Chicago/Turabian StyleNúñez, Claudia, Ronald Nelson, Gerald Tabilo, Paulina Pefaur, Rodrigo Castillo, and Alifhers Mestra. 2024. "Simultaneous Detection of Serotonin and 17β-Estradiol Using rGO/SPCE Modified with Cu(II) Complex: A Novel Approach for PMDD Diagnosis" Chemosensors 12, no. 8: 164. https://doi.org/10.3390/chemosensors12080164