Epithelial Cell Adhesion Molecule (EpCAM) Expression Can Be Modulated via NFκB
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patient Samples
2.2. RNA Isolation
2.3. Cell Culture
2.4. Cell Viability Assay
2.5. In Vitro Treatment of Jurkat Cells with Costunolide
2.6. Quantitative Real Time PCR
2.7. Measurement of Telomere Length
2.8. Docking of Costunolide with EpCAM and Myc Proteins
2.9. Computational Analysis
2.10. Statistical Analysis
3. Results
3.1. Association of EpCAM Gene Expression with Demographic Data
3.2. Effect of Costunolide on Jurkat Cell Viability
3.3. Effect of Costunolide on Genes (EpCAM, Myc and TERT) Expression and Telomere Length
3.4. In-Silico Interaction of Costunolide with EpCAM and Myc
3.5. Identification of Transcription Factor Binding Sites within EpCAM, Myc, and TERT Promoter Region
3.6. Costunolide Reduced NFκB Expression
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cases vs. Control | ||||||
---|---|---|---|---|---|---|
Pediatric | Adults | |||||
Characteristics | Controls | Cases | p Value | Controls | Cases | p Value |
Subjects (N) | 50 | 153 | - | 39 | 62 | - |
Age (years) | 2.77 (3.25) | 8.58 (4.92) | 0.0026 | 30.0 (9.04) | 31.3 (9.12) | 0.5983 |
Sex (Male) (%) | 31 (62.0%) | 100 (65.4%) | 0.830 | 31 (79.5%) | 49 (79.0%) | 0.797 |
ALL immunophenotypes | ||||||
B-cell ALL117 (76.5%)45 (72.5%)0.3413 | ||||||
T-cell ALL36 (23.5%)17 (27.7%)0.9218 |
NFκB Subunits | EpCAM | Myc | TERT |
---|---|---|---|
NFκB1/p50 | −916, −691, −134 bps | −161, −7 bps | −742, −727, −694, −692 bps |
p65/RelA | −393 bps | −259 bps | −599, −598 bps |
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Zia, S.; Tehreem, K.; Batool, S.; Ishfaq, M.; Mirza, S.B.; Khan, S.; Almashjary, M.N.; Hazzazi, M.S.; Qanash, H.; Shaikh, A.; et al. Epithelial Cell Adhesion Molecule (EpCAM) Expression Can Be Modulated via NFκB. Biomedicines 2022, 10, 2985. https://doi.org/10.3390/biomedicines10112985
Zia S, Tehreem K, Batool S, Ishfaq M, Mirza SB, Khan S, Almashjary MN, Hazzazi MS, Qanash H, Shaikh A, et al. Epithelial Cell Adhesion Molecule (EpCAM) Expression Can Be Modulated via NFκB. Biomedicines. 2022; 10(11):2985. https://doi.org/10.3390/biomedicines10112985
Chicago/Turabian StyleZia, Saadiya, Komal Tehreem, Sidra Batool, Mehreen Ishfaq, Shaher Bano Mirza, Shahrukh Khan, Majed N. Almashjary, Mohannad S. Hazzazi, Husam Qanash, Ahmad Shaikh, and et al. 2022. "Epithelial Cell Adhesion Molecule (EpCAM) Expression Can Be Modulated via NFκB" Biomedicines 10, no. 11: 2985. https://doi.org/10.3390/biomedicines10112985