Conformational Dynamics of the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein
Abstract
:1. Introduction
2. Materials and Methods
2.1. P4A1 and RBD Variants Production and Purification
2.2. Binding Kinetics
2.3. RBD Protein Models
2.4. Molecular Dynamics
2.5. Dihedral Angle Principal Component Analysis (dPCA)
3. Results and Discussion
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Mamchur, A.A.; Stanishneva-Konovalova, T.B.; Mokrushina, Y.A.; Abrikosova, V.A.; Guo, Y.; Zhang, H.; Terekhov, S.S.; Smirnov, I.V.; Yaroshevich, I.A. Conformational Dynamics of the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein. Biomedicines 2022, 10, 3233. https://doi.org/10.3390/biomedicines10123233
Mamchur AA, Stanishneva-Konovalova TB, Mokrushina YA, Abrikosova VA, Guo Y, Zhang H, Terekhov SS, Smirnov IV, Yaroshevich IA. Conformational Dynamics of the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein. Biomedicines. 2022; 10(12):3233. https://doi.org/10.3390/biomedicines10123233
Chicago/Turabian StyleMamchur, Aleksandra A., Tatiana B. Stanishneva-Konovalova, Yuliana A. Mokrushina, Viktoria A. Abrikosova, Yu Guo, Hongkai Zhang, Stanislav S. Terekhov, Ivan V. Smirnov, and Igor A. Yaroshevich. 2022. "Conformational Dynamics of the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein" Biomedicines 10, no. 12: 3233. https://doi.org/10.3390/biomedicines10123233