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Volume 10
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Article

Human Hair Follicle-Derived Mesenchymal Stromal Cells from the Lower Dermal Sheath as a Competitive Alternative for Immunomodulation

by
Beatriz Hernaez-Estrada
1,2,
Ainhoa Gonzalez-Pujana
2,3,4,
Andoni Cuevas
5,
Ander Izeta
6,7,
Kara L. Spiller
1,
Manoli Igartua
2,3,4,
Edorta Santos-Vizcaino
2,3,4,*,† and
Rosa Maria Hernandez
2,3,4,*,†
1
School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104, USA
2
NanoBioCel Research Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain
3
Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Institute of Health Carlos III, 28029 Madrid, Spain
4
Bioaraba, NanoBioCel Research Group, 01006 Vitoria-Gasteiz, Spain
5
Clínica Ercilla, 48009 Bilbao, Spain
6
Tissue Engineering Group, Biodonostia Health Research Institute, 20014 Donostia-San Sebastián, Spain
7
Department of Biomedical Engineering and Sciences, School of Engineering, Tecnun-University of Navarra, 20009 Donostia-San Sebastián, Spain
*
Authors to whom correspondence should be addressed.
†
These authors equally contributed to this work.
Biomedicines 2022, 10(2), 253; https://doi.org/10.3390/biomedicines10020253
Submission received: 8 December 2021 / Revised: 1 January 2022 / Accepted: 14 January 2022 / Published: 24 January 2022
(This article belongs to the Section Cell Biology and Pathology)
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Abstract

Mesenchymal stromal cells (MSCs) have unique immunomodulatory capacities. We investigated hair follicle-derived MSCs (HF-MSCs) from the dermal sheath, which are advantageous as an alternative source because of their relatively painless and minimally risky extraction procedure. These cells expressed neural markers upon isolation and maintained stemness for a minimum of 10 passages. Furthermore, HF-MSCs showed responsiveness to pro-inflammatory environments by expressing type-II major histocompatibility complex antigens (MHC)-II to a lesser extent than adipose tissue-derived MSCs (AT-MSCs). HF-MSCs effectively inhibited the proliferation of peripheral blood mononuclear cells equivalently to AT-MSCs. Additionally, HF-MSCs promoted the induction of CD4+CD25+FOXP3+ regulatory T cells to the same extent as AT-MSCs. Finally, HF-MSCs, more so than AT-MSCs, skewed M0 and M1 macrophages towards M2 phenotypes, with upregulation of typical M2 markers CD163 and CD206 and downregulation of M1 markers such as CD64, CD86, and MHC-II. Thus, we conclude that HF-MSCs are a promising source for immunomodulation.
Keywords: immunomodulation; hair follicle; MSCs; macrophages; regulatory T cells; PBMC; immunoevasive immunomodulation; hair follicle; MSCs; macrophages; regulatory T cells; PBMC; immunoevasive
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MDPI and ACS Style

Hernaez-Estrada, B.; Gonzalez-Pujana, A.; Cuevas, A.; Izeta, A.; Spiller, K.L.; Igartua, M.; Santos-Vizcaino, E.; Hernandez, R.M. Human Hair Follicle-Derived Mesenchymal Stromal Cells from the Lower Dermal Sheath as a Competitive Alternative for Immunomodulation. Biomedicines 2022, 10, 253. https://doi.org/10.3390/biomedicines10020253

AMA Style

Hernaez-Estrada B, Gonzalez-Pujana A, Cuevas A, Izeta A, Spiller KL, Igartua M, Santos-Vizcaino E, Hernandez RM. Human Hair Follicle-Derived Mesenchymal Stromal Cells from the Lower Dermal Sheath as a Competitive Alternative for Immunomodulation. Biomedicines. 2022; 10(2):253. https://doi.org/10.3390/biomedicines10020253

Chicago/Turabian Style

Hernaez-Estrada, Beatriz, Ainhoa Gonzalez-Pujana, Andoni Cuevas, Ander Izeta, Kara L. Spiller, Manoli Igartua, Edorta Santos-Vizcaino, and Rosa Maria Hernandez. 2022. "Human Hair Follicle-Derived Mesenchymal Stromal Cells from the Lower Dermal Sheath as a Competitive Alternative for Immunomodulation" Biomedicines 10, no. 2: 253. https://doi.org/10.3390/biomedicines10020253

APA Style

Hernaez-Estrada, B., Gonzalez-Pujana, A., Cuevas, A., Izeta, A., Spiller, K. L., Igartua, M., Santos-Vizcaino, E., & Hernandez, R. M. (2022). Human Hair Follicle-Derived Mesenchymal Stromal Cells from the Lower Dermal Sheath as a Competitive Alternative for Immunomodulation. Biomedicines, 10(2), 253. https://doi.org/10.3390/biomedicines10020253

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