Testicular Macrophages Produce Progesterone De Novo Promoted by cAMP and Inhibited by M1 Polarization Inducers
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Propagation of Tissue-Resident Mø by Mixed Culturing with Interstitial Cells Obtained from the Testes
2.3. Separation of Testicular Tissue-Resident Mø Propagated by Mixed Culture from Interstitial Cells
2.4. Phagocytosis Analysis with Fluorescent Beads
2.5. Flow Cytometry
2.6. Immunofluorescence Staining
2.7. Total RNA Extraction and RT-PCR Analyses
2.8. P4 Measurement
2.9. qPCR Analysis
3. Results
3.1. Propagation of Testicular Tissue-Resident Mø in Mixed Culture with Interstitial Cells Rich in Leydig Cells and Segregation of Mø by Adhesion to the Bacteriological Petri Dish
3.2. Mø Marker Expression Profiles by Flow Cytometry in Propagated Testicular Mø with the Mixed Culture
3.3. Expression Profiles of Transcription Factors Shaping Mø and Molecules Involved in Spermatogenesis and Mø Properties in Propagated Testicular Mø
3.4. Properties of Testicular Interstitial Cells Propagated by Mixed Culture with Testicular Mø
3.5. Localization of Testicular Mø Exhibiting Properties of Steroid Production
3.6. P4 production in Testicular Mø Propagated in Mixed Culture
3.7. P4 Production Upregulation in Testicular Mø by cAMP and Iso
3.8. P4 Production in Testicular Mø by M1 Polarization Inducers
4. Discussion
4.1. Propagation of Testicular Interstitial Mø in Mixed Culture
4.2. De Novo P4 Production by Testicular Interstitial Mø
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yamauchi, S.; Yamamoto, K.; Ogawa, K. Testicular Macrophages Produce Progesterone De Novo Promoted by cAMP and Inhibited by M1 Polarization Inducers. Biomedicines 2022, 10, 487. https://doi.org/10.3390/biomedicines10020487
Yamauchi S, Yamamoto K, Ogawa K. Testicular Macrophages Produce Progesterone De Novo Promoted by cAMP and Inhibited by M1 Polarization Inducers. Biomedicines. 2022; 10(2):487. https://doi.org/10.3390/biomedicines10020487
Chicago/Turabian StyleYamauchi, Sawako, Kousuke Yamamoto, and Kazushige Ogawa. 2022. "Testicular Macrophages Produce Progesterone De Novo Promoted by cAMP and Inhibited by M1 Polarization Inducers" Biomedicines 10, no. 2: 487. https://doi.org/10.3390/biomedicines10020487