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Biomedicines
Volume 10
Issue 3
10.3390/biomedicines10030633
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Article

Describing Characteristics and Differences of Neutrophils in Sepsis, Trauma, and Control Patients in Routinely Measured Hematology Data

by
Huibert-Jan Joosse
1,
Albert Huisman
1,
Wouter van Solinge
1,
Falco Hietbrink
2,
Imo Hoefer
1 and
Saskia Haitjema
1,*
1
Central Diagnostic Laboratory, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands
2
Department of Trauma Surgery, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands
*
Author to whom correspondence should be addressed.
Biomedicines 2022, 10(3), 633; https://doi.org/10.3390/biomedicines10030633
Submission received: 31 January 2022 / Revised: 1 March 2022 / Accepted: 7 March 2022 / Published: 9 March 2022
(This article belongs to the Special Issue Neutrophils, Fast and Strong)
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Abstract

Neutrophils have an important role in the immune response. These cells can be subjected to an impaired function and a shift in population depending on disease states. In sepsis, this shift is recognized and flagged by automated hematology analyzers, including the presence of band neutrophils, while these cells, although present, appear not to be detected in trauma patients. To better understand this suspected error in flagging, we set out to distinguish neutrophil populations of these two patient groups and compared these with controls. Different data-driven methods were used compared to standard algorithms used by the software of the analyzers. Using K-means clustering, we extracted neutrophils from raw hematology analyzer datafiles, and compared characteristics of these clusters between the patient groups. We observed an increased neutrophil size for both sepsis and trauma patients, but trauma patients had a smaller increase. Trauma patients also had a high proportion of cells with relatively high nuclear segmentation, which is contradictory with the presence of band neutrophils. This, in combination with the smaller size increase, might explain the inability to flag band neutrophils in trauma.
Keywords: neutrophils; sepsis; trauma; bands; clustering neutrophils; sepsis; trauma; bands; clustering

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MDPI and ACS Style

Joosse, H.-J.; Huisman, A.; van Solinge, W.; Hietbrink, F.; Hoefer, I.; Haitjema, S. Describing Characteristics and Differences of Neutrophils in Sepsis, Trauma, and Control Patients in Routinely Measured Hematology Data. Biomedicines 2022, 10, 633. https://doi.org/10.3390/biomedicines10030633

AMA Style

Joosse H-J, Huisman A, van Solinge W, Hietbrink F, Hoefer I, Haitjema S. Describing Characteristics and Differences of Neutrophils in Sepsis, Trauma, and Control Patients in Routinely Measured Hematology Data. Biomedicines. 2022; 10(3):633. https://doi.org/10.3390/biomedicines10030633

Chicago/Turabian Style

Joosse, Huibert-Jan, Albert Huisman, Wouter van Solinge, Falco Hietbrink, Imo Hoefer, and Saskia Haitjema. 2022. "Describing Characteristics and Differences of Neutrophils in Sepsis, Trauma, and Control Patients in Routinely Measured Hematology Data" Biomedicines 10, no. 3: 633. https://doi.org/10.3390/biomedicines10030633

APA Style

Joosse, H.-J., Huisman, A., van Solinge, W., Hietbrink, F., Hoefer, I., & Haitjema, S. (2022). Describing Characteristics and Differences of Neutrophils in Sepsis, Trauma, and Control Patients in Routinely Measured Hematology Data. Biomedicines, 10(3), 633. https://doi.org/10.3390/biomedicines10030633

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