The Effect of Heat Treatment of β-Tricalcium Phosphate-Containing Silica-Based Bioactive Aerogels on the Cellular Metabolism and Proliferation of MG63 Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis of β-TCP-Modified Aerogels
2.2. Nitrogen Adsorption–Desorption Porosimetry
2.3. Grinding and Microscopic Study of β-TCP-Modified Aerogels (Aerogel Composites)
2.4. Determination of the Compressive Strength Values
2.5. Structural Study of as-Prepared and Heat-Treated Samples
2.6. Dissolution Dynamics and Ion-Release Properties of the Heat-Treated Composites
2.7. Cell Culture Studies
2.8. Scanning Electron Microscopy (SEM) Analysis of Structural Properties and Cell Growth
2.9. Cell Viability Assay
2.10. Alkaline Phosphatase Activity Assay
2.11. Gene Expression Analysis
2.12. Statistical Analysis
3. Results
3.1. Structural Studies
3.2. Cell Viability Assay
3.3. Alkaline Phosphatase Activity Assay
3.4. Gene Expression Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Temperature | BET Surface Area (m2/g) | C-Constant | Average Pore Diameter (nm) | Pore Volume (cm3/g) | Micropore Volume (cm3/g) |
---|---|---|---|---|---|
500 | 400.0 | 82.46 | 26.34 | 3.397 | 0.026 |
600 | 415.0 | 56.33 | 33.98 | 3.254 | 0.021 |
700 | 382.8 | 64.81 | 33.97 | 3.251 | 0.000 |
800 | 384.9 | 59.40 | 31.95 | 3.074 | 0.021 |
900 | 277.0 | 51.18 | 46.73 | 3.237 | 0.005 |
1000 | 184.4 | 52.03 | 29.59 | 1.363 | 0.010 |
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Hegedűs, C.; Czibulya, Z.; Tóth, F.; Dezső, B.; Hegedűs, V.; Boda, R.; Horváth, D.; Csík, A.; Fábián, I.; Tóth-Győri, E.; et al. The Effect of Heat Treatment of β-Tricalcium Phosphate-Containing Silica-Based Bioactive Aerogels on the Cellular Metabolism and Proliferation of MG63 Cells. Biomedicines 2022, 10, 662. https://doi.org/10.3390/biomedicines10030662
Hegedűs C, Czibulya Z, Tóth F, Dezső B, Hegedűs V, Boda R, Horváth D, Csík A, Fábián I, Tóth-Győri E, et al. The Effect of Heat Treatment of β-Tricalcium Phosphate-Containing Silica-Based Bioactive Aerogels on the Cellular Metabolism and Proliferation of MG63 Cells. Biomedicines. 2022; 10(3):662. https://doi.org/10.3390/biomedicines10030662
Chicago/Turabian StyleHegedűs, Csaba, Zsuzsanna Czibulya, Ferenc Tóth, Balázs Dezső, Viktória Hegedűs, Róbert Boda, Dóra Horváth, Attila Csík, István Fábián, Enikő Tóth-Győri, and et al. 2022. "The Effect of Heat Treatment of β-Tricalcium Phosphate-Containing Silica-Based Bioactive Aerogels on the Cellular Metabolism and Proliferation of MG63 Cells" Biomedicines 10, no. 3: 662. https://doi.org/10.3390/biomedicines10030662