Retinal Changes in Astrocytes and Müller Glia in a Mouse Model of Laser-Induced Glaucoma: A Time-Course Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Experimental Groups
2.3. Anesthetics
2.4. Laser Treatment and Measurement of IOP
2.5. Immunohistochemistry
2.6. Quantitative Retinal Analysis
2.6.1. GFAP-RA
2.6.2. GFAP-IRI and MHC-II-IRI
2.7. Statistical Analysis
3. Results
3.1. Intraocular Pressure (IOP)
3.2. General Characteristics of Astrocytes and Müller Glia in the Naïve Retina
3.3. Morphological Changes in Retinal Macroglia of Contralateral Eyes at Different Time Points after OHT Induction
3.4. Morphological Changes in Retinal Macroglia of OHT Eyes at Different Time Points after OHT Induction
3.5. GFAP-Labeled Retinal Area at Different Time Points after OHT Induction
3.5.1. GFAP-RA Total Value
3.5.2. GFAP-RA Values by Retinal Sectors
- -
- Superior retina: (i) the S-OD at 1, 3, 5 and 8 (all p < 0.01) days; (ii) the S-IR at 1, 3, 5, 8 (all p < 0.01) and 15 (p < 0.05) days; (iii) the S-PR at all the time points analyzed (p < 0.01).
- -
- Inferior retina: (i) the I-OD at 1 (p < 0.05), 5 (p < 0.01) and 8 (p < 0.05) days; (ii) the I-IR at 1, 3 (p < 0.05), 5 (p < 0.01) and 8 (p < 0.05) days; (iii) the I-PR at 1, 3, 5 (p < 0.05) and 8 (p < 0.01) days.
- -
- Nasal retina: (i) the N-OD at 1 (p < 0.01), 3 (p < 0.05), 5 (p < 0.01) and 8 (p < 0.05) days; (ii) the N-IR at 1, 3, 5, 8 (p < 0.01) and 15 (p < 0.05) days; (iii) the N-PR at 1, 3, 5 (p < 0.01) and 8 (p < 0.05) days.
- -
- Temporal retina: (i) the T-OD only at 5 days (p < 0.05); (ii) the T-IR at 1, 3, 5 and 8 (p < 0.01) days; (iii) the T-PR at 1, 3, 5 and 8 (p < 0.01) days.
- -
- Superior retina: (i) the S-OD at 1, 3, 5 (all p < 0.01) and 8 (p < 0.05) days; (ii) the S-IR at 1, 3, 5 and 8 days (all p < 0.01); (iii) the S-PR at 1, 3, 5 and 8 (p < 0.01) days.
- -
- Inferior retina: (i) the I-OD at 3 days (p < 0.01); (ii) the I-IR at 1 and 3 days (both p < 0.01) and at 5 and 8 days (p < 0.05); (iii) the I-PR at 3 (p < 0.01), 5 and 8 (both p < 0.05) days.
- -
- Nasal retina: (i) the N-OD at 1 (p < 0.05), 3 (p < 0.01) and 5 (p < 0.05) days; (ii) the N-IR at 1, 3, 5 and 8 days (all p < 0.01); (iii) the N-PR at 1, 3 (both p < 0.01), 5 (p < 0.05) and 8 (p < 0.01) days. At 15 days, in the N-IR, we found a significant decrease in GFAP-RA (p < 0.05).
- -
- Temporal retina: (i) the T-OD only at Day 3 (p < 0.01); (ii) the T-IR at 1, 3, 5 and 8 days (all p < 0.01); (iii) the T-PR at 1 (p < 0.05), 3, 5 and 8 (all p < 0.01) days.
3.6. Intensity of GFAP Immunoreactivity at Different Time Points after OHT Induction
- -
- Superior retina: (i) the S-IR at 3, 5 and 8 days (all p < 0.05); (ii) the S-PR at 1 (p < 0.05), 3, 5, 8 (all p < 0.01) and 15 (p < 0.05) days.
- -
- Inferior retina: (i) the I-OD at 15 days (p < 0.05); (ii) the I-IR at 1, 3 and 8 days (all p < 0.05); (iii) the I-PR at all the time points analyzed (p < 0.05).
- -
- Nasal retina: (i) the N-IR at 1, 3, and 8 days (all p < 0.05); (ii) the N-PR at all the time points analyzed (p < 0.05).
- -
- Temporal retina: (i) the T-OD at 15 days (p < 0.05); (ii) the T-PR at 3, 5, (p < 0.01), 8 (p < 0.05) and 15 (p < 0.01) days.
- -
- Superior retina: (i) a decrease in the S-OD at 1 day (p < 0.05); (ii) an increase in the S-IR at 1 and 3 (p < 0.05) days; (iii) an increase in the S-PR at 1, 3, 5 (p < 0.05) and 8 (p < 0.01) days.
- -
- Inferior retina: (i) a decrease in the I-OD at 5 days (p < 0.05); (ii) an increase in the I-IR at 8 (p < 0.05) days; (iii) an increase in the I-PR at 1, 3, 8 and 15 (p < 0.05) days.
- -
- Nasal retina: (i) an increase in the N-IR at 5 and 8 (p < 0.05) days; (ii) an increase in the N-PR at 1, 3, 5 and 8 (p < 0.05) days.
- -
- Temporal retina: (i) a decrease in the T-OD at 5 days (p < 0.05); (ii) an increase in the T-IR at 1 day (p < 0.05); (iii) an increase in the T-PR at 1 day (p < 0.01), 3 days (p < 0.05) and 8 days (p < 0.01).
3.7. Intensity of MHC-II Immunoreactivity at Different Time Points after OHT Induction
- -
- Superior retina: (i) the S-OD only at 3 days (p < 0.05); (ii) the S-IR at 1, 3, 5 (all p < 0.01) and 8 (p < 0.05) days; (iii) the S-PR at all the time points analyzed (all p < 0.01).
- -
- Inferior retina: (i) I-IR at 1 (p < 0.01), 3 (p < 0.05), 5 and 8 (p < 0.01) days; (ii) the I-PR at all the time points analyzed (all p < 0.01).
- -
- Nasal retina: (i) the N-IR at all the time points analyzed (all p < 0.01); (ii) the N-PR at all the time points analyzed (all p < 0.01).
- -
- Temporal retina: (i) the T-OD only at 8 days (p < 0.05); (ii) the T-IR at all the time points analyzed (all p < 0.01); (iii) the T-PR at all the time points analyzed (all p < 0.01).
- -
- Superior retina: (i) the S-IR at 1 (p < 0.05), 3, 5 (both p < 0.01) and 8 (p < 0.05) days; (ii) the S-PR at all the time points analyzed (all p < 0.01).
- -
- Inferior retina: (i) the I-IR at 3 (p < 0.01) and 8 (p < 0.05) days; (ii) the I-PR at 1 (p < 0.05), 3 (p < 0.01), 5 and 8 (both p < 0.05) days.
- -
- Nasal retina: (i) the N-IR at 3 (p < 0.01), 5 and 8 (both p < 0.05) days; (ii) the N-PR at 1, 3 (both p < 0.01), 5 and 8 (p < 0.05) days.
- -
- Temporal retina: (i) the T-IR at 3, 5 and 8 (all p < 0.05) days; (ii) the T-PR at 1, 3 (both p < 0.01), 5 (p < 0.05) and 8 (p < 0.01) days.
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Fernández-Albarral, J.A.; de Hoz, R.; Matamoros, J.A.; Chen, L.; López-Cuenca, I.; Salobrar-García, E.; Sánchez-Puebla, L.; Ramírez, J.M.; Triviño, A.; Salazar, J.J.; et al. Retinal Changes in Astrocytes and Müller Glia in a Mouse Model of Laser-Induced Glaucoma: A Time-Course Study. Biomedicines 2022, 10, 939. https://doi.org/10.3390/biomedicines10050939
Fernández-Albarral JA, de Hoz R, Matamoros JA, Chen L, López-Cuenca I, Salobrar-García E, Sánchez-Puebla L, Ramírez JM, Triviño A, Salazar JJ, et al. Retinal Changes in Astrocytes and Müller Glia in a Mouse Model of Laser-Induced Glaucoma: A Time-Course Study. Biomedicines. 2022; 10(5):939. https://doi.org/10.3390/biomedicines10050939
Chicago/Turabian StyleFernández-Albarral, Jose A., Rosa de Hoz, José A. Matamoros, Lejing Chen, Inés López-Cuenca, Elena Salobrar-García, Lidia Sánchez-Puebla, José M. Ramírez, Alberto Triviño, Juan J. Salazar, and et al. 2022. "Retinal Changes in Astrocytes and Müller Glia in a Mouse Model of Laser-Induced Glaucoma: A Time-Course Study" Biomedicines 10, no. 5: 939. https://doi.org/10.3390/biomedicines10050939