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Article

Genomic-Analysis-Oriented Drug Repurposing in the Search for Novel Antidepressants

by
Mohammad Hendra Setia Lesmana
1,
Nguyen Quoc Khanh Le
2,3,4,
Wei-Che Chiu
5,6,
Kuo-Hsuan Chung
7,8,
Chih-Yang Wang
9,10,
Lalu Muhammad Irham
11,* and
Min-Huey Chung
1,12,*
1
School of Nursing, College of Nursing, Taipei Medical University, Taipei 11031, Taiwan
2
Professional Master Program in Artificial Intelligence in Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
3
Research Center for Artificial Intelligence in Medicine, Taipei Medical University, Taipei 11031, Taiwan
4
Translational Imaging Research Center, Taipei Medical University Hospital, Taipei 11031, Taiwan
5
Department of Psychiatry, Cathay General Hospital, Taipei 10630, Taiwan
6
School of Medicine, Fu Jen Catholic University, New Taipei City 242062, Taiwan
7
Department of Psychiatry, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
8
Department of Psychiatry and Psychiatric Research Center, Taipei Medical University Hospital, Taipei Medical University, Taipei 11031, Taiwan
9
Ph.D. Program for Cancer Molecular Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University and Academia Sinica, Taipei 11031, Taiwan
10
Graduate Institute of Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan
11
Faculty of Pharmacy, University of Ahmad Dahlan, Yogyakarta 55164, Indonesia
12
Department of Nursing, Shuang Ho Hospital, Taipei Medical University, New Taipei City 23561, Taiwan
 
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*
Authors to whom correspondence should be addressed.
Biomedicines 2022, 10(8), 1947; https://doi.org/10.3390/biomedicines10081947
Submission received: 20 June 2022 / Revised: 7 August 2022 / Accepted: 8 August 2022 / Published: 11 August 2022
(This article belongs to the Section Drug Discovery, Development and Delivery)
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Abstract

:
From inadequate prior antidepressants that targeted monoamine neurotransmitter systems emerged the discovery of alternative drugs for depression. For instance, drugs targeted interleukin 6 receptor (IL6R) in inflammatory system. Genomic analysis-based drug repurposing using single nucleotide polymorphism (SNP) inclined a promising method for several diseases. However, none of the diseases was depression. Thus, we aimed to identify drug repurposing candidates for depression treatment by adopting a genomic-analysis-based approach. The 5885 SNPs obtained from the machine learning approach were annotated using HaploReg v4.1. Five sets of functional annotations were applied to determine the depression risk genes. The STRING database was used to expand the target genes and identify drug candidates from the DrugBank database. We validated the findings using the ClinicalTrial.gov and PubMed databases. Seven genes were observed to be strongly associated with depression (functional annotation score = 4). Interestingly, IL6R was auspicious as a target gene according to the validation outcome. We identified 20 drugs that were undergoing preclinical studies or clinical trials for depression. In addition, we identified sarilumab and satralizumab as drugs that exhibit strong potential for use in the treatment of depression. Our findings indicate that a genomic-analysis-based approach can facilitate the discovery of drugs that can be repurposed for treating depression.

Graphical Abstract

1. Introduction

Depression is an emerging mental health problem affecting 322 million people around the world. Southeast Asia and the Western Pacific are the regions where depression is most prevalent [1]. Recent studies conducted in Taiwan reported that the prevalence of depression was 3.7–24.1% [2,3]. Some factors are classed as risk factors of depression, including genetic variation (single-nucleotide polymorphisms; SNPs) [4], female gender [5], chronic diseases [6], stressful life experiences [7], unemployment [8], low education level [8,9], poor physical exercise [10], lack of social support [11], and poor environment (high pollution) [12]. Furthermore, depression has been associated with poor quality of life, lack of well-being, disrupted daily activities, higher healthcare expenditure and utilization, and economic burden [13,14,15]. Thus, depression treatment remains one of the top public health priorities.
In terms of pathological neural substrates associated with depression, some brain regions have been discovered to be responsible for emotion and behavior [16]. The prefrontal cortex (PFC) is the highest level of the cerebral hierarchy, and is in charge of representing and carrying out actions [16]. The ventromedial cortex is a part of the PFC that has the function of controlling emotions resulting from autonomic and neuroendocrine system impulses [17]. A meta-analysis described that the roles of PFC, amygdala, and the hippocampus were crucial for depression development [18]. Increased amygdala activity was observed to be a precursor to depression [19]. In addition, prior studies have reported that the severity of depression is correlated with abnormal amygdala size and function [20,21]. Meanwhile, the hippocampus role has been excessively investigated due to its structure, which is rich in corticosteroid receptors, and its involvement in learning, memory, and neurogenesis processes [22,23]. A previous study described the association between the hippocampus and the hypothalamic–pituitary–adrenal (HPA) axis, in which negative impulses of stress lead to HPA axis activation by reducing neuronal plasticity in the hippocampus, resulting in a high level of cortisol, as one of the indicators of depression [24]. Moreover, according to Stawski et al., cortisol levels have been investigated in terms of their contribution to depression-related cognitive impairment [25]. Several studies also found that increasing cortisol secretion is associated with cognitive decline [26,27].
Barchas and Altemus proposed that the underlying pathomechanism for depression is related to the monoamine hypothesis [28]. This hypothesis postulates that the diminished availability of three key monoamine neurotransmitters (serotonin, norepinephrine, and dopamine) causes decreased neurotransmission and worsened cognitive function, both of which may contribute to depression [28]. Meanwhile, in the past decades, this hypothesis does not adequately explain the pathogenesis of depression [29]. Numerous studies point out the involvement of inflammatory processes in depression development [30,31,32]. The circulating of pro-inflammatory mediators in the brain is the consequence of the weakening of the semipermeable border of blood–brain barrier (BBB) due to chronical stress stimuli [33]. In depression, pro-inflammatory mediators such as IL6 have been linked to increasing the activity of the HPA axis by inducing the release of corticoliberin-releasing hormone (CRH) from the hypothalamus [34,35,36]. In term of serotonin synthesis, pro-inflammatory mediators (IL6, TNF, CRP) induced the activity of the HPA axis and an enzyme of tryptophan metabolism (indoleamine 2,3-dioxygenase), which led to lower levels of tryptophan and serotonin [36,37]. Prior studies have proposed that elevated IL6 and C-reactive protein (CRP) levels might predict the development of depressive symptoms [38,39,40]. Therefore, the exploration of the inflammatory hypothesis in depression raises the possibility of there being other biological processes that contribute to depression development, and opens the door to improving the treatment of depression.
The current treatment of depression is antidepressants, which was developed on the basis of monoamine neurotransmitter systems and target neural synapses [41]. Monoamine oxidase inhibitors (MAOIS) and tricyclic antidepressants are the first generation medications for depression; these produce serious adverse effects by blocking postsynaptic receptors [42]. For safety reasons, the second generation of antidepressants was developed, which includes selective serotonin reuptake inhibitors (SSRIs), selective noradrenaline reuptake inhibitors (SNRIs), dual serotonin and noradrenaline reuptake inhibitors, and multitarget antidepressants [42]. According to the National Institute for Clinical Excellence, SSRI medications are recommended to be the first-line treatment of depression [43]. However, SSRI medication often induces insufficient responses. Only 30% of individuals take commonly recommended antidepressant medications for depression remission [44], while 15–60% of depressed patients do not adequately response the medication [45]. Three in every ten patients with depression that are treated with antidepressants have reported treatment resistance [46]. Therefore, discovering alternative targets and potential medications for treating depression is urgent.
Drug repurposing is a common method for identifying potential new treatments using existing drugs [47,48]. The term “drug repurposing” refers to the repositioning of an existing medicine for a new indication [49]. For example, ketamine was originally approved by the United States Food and Drug Administration (USFDA) in 1970 for use as an intravenous anesthesia agent, but in 2019, it was approved for a new application: treatment-resistant depression [50,51]. Drug repurposing has some advantages over the conventional method of drug discovery; for example, drug repurposing candidates have already passed clinical trials for the original indication, and drug repurposing is faster and cheaper than the conventional method [7]. Furthermore, the mechanisms through which repurposed drugs affect the human body are usually already well established [47,52]. Therefore, the safety issues of repurposed drugs have been passed for the use in new medication.
Recent technological developments have encouraged researchers to consider common genetic variants, such as single-nucleotide polymorphisms (SNPs), in drug repurposing [53]. A popular method, established by Okada, et al. [54], involves utilizing a scoring system comprising eight functional annotations based on genomic analysis to prioritize target genes and discover drug repurposing candidates; the method was originally used to identify candidates for the treatment of rheumatoid arthritis according to SNP data collected from genome-wide association studies. Other studies have adapted Okada’s approach to use five sets of functional annotations to discover drug repurposing candidates for the treatment of atopic dermatitis [55] and asthma [56]. Functional annotations are considered crucial for evaluating diseases. Missense variants are nonsynonymous single-base changes that can cause changes in proteins [57]. Cis expression quantitative trait loci (cis-eQTL) are used to observed the variation in expressed genes in various tissues [58]. The Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations are based on observing genetic associations that have an important role in molecular pathways [58]. Molecular pathway analysis related with protein–protein interactions (PPIs) consists of observing gene contributions to molecular functions in an organism [59]. Knockout mouse phenotype (KO mice) annotations exhibit considerable overlap with mammalian phenotype (MP) ontology annotations [60]. Accordingly, we postulate that a genomic-analysis-based approach using functional annotations could facilitate the discovery of candidates for drug repurposing for the treatment of depression.
Few studies have used SNP data to discover new drugs and drug repurposing candidates for the treatment of depression. A previous study involving the development of new drugs for treating major depressive disorder (MDD) focused only on genetic drug–target networks [61]. However, no study has adopted the genomic-analysis-based approach using functional annotations to identify drug repurposing candidates for the treatment of depression. In the present study, we prioritized potential target genes and drug repurposing candidates for depression by integrating SNP data from the Taiwan Biobank database with a machine learning algorithm by adopting a genomic-analysis-based approach and five sets of functional annotations (missense variant, cis-eQTL, KEGG, PPI, and KO mice).

2. Materials and Methods

2.1. Study Design

A descriptive schematic of the present study is presented in Figure 1. The SNPs were queried from the Taiwan Biobank dataset using an Extreme Gradient Boost (XGBoost) machine learning algorithm. SNPs connected to other SNPs in the network were retained. Next, we performed functional annotation of the SNPs according to the five aforementioned sets of functional annotations (missense, cis-eQTL, KEGG, PPI, and KO mice) using HaploReg V4.1. The prioritization of depression-associated genes was based on a scoring system comprising the five sets of functional annotations. The genes that were prioritized and identified as depression risk genes were converted and extended using the STRING database. Thereafter, overlapping of gene targets and drugs was identified using the DrugBank database. Finally, validation was performed using ClinicalTrials.gov and PubMed for drugs that were undergoing clinical trials and preclinical (in vitro and in vivo) studies, respectively.

2.2. Genes Associated with Depression

The SNPs identified using the machine learning algorithm were input into HaploReg v4.1 for functional annotation [62]. HaploReg v4.1 provides thorough information regarding genomic variants and changes in proteins by integrating various functional annotations [62]. Accordingly, the SNPs that encoded the genes for depression were obtained, and the list of the genes was used in subsequent analyses.

2.3. Five Sets of Functional Annotations for Prioritizing Genes Associated with Depression

A scoring system indicating the most promising target genes integrating the five sets of functional annotations was constructed. The sets of functional annotations were as follows: (i) Missense, to conduct missense functional annotations, we used RStudio v3.4.3 and the HaploR package [63], which contains annotations of functional consequences from a database of SNPs (dbSNPs). Because changes in the amino acid sequences might alter protein function, missense or nonsense variants can be considered as one of the important functional annotations. The genes with missense SNPs associated with depression were assigned 1 point. (ii) cis-eQTLs, a cis-eQTL SNP affects the expression of the gene at the location of the SNP [64]. The SNP is linked to a shift in gene expression in the target tissue, which has physiological consequences. Any gene with a cis-eQTL SNP associated with depression expressed in whole blood was given 1 point. (iii) KEGG, the KEGG, an online biochemical route database, was used to perform molecular pathway enrichment analysis [65]. Genes that were abundant in the KEGG pathway (false discovery rate (FDR) of 0.05) were each assigned 1 point [66]. (iv) PPI, the biological process category of gene ontology was used as a data source. An FDR of 0.05 was established as the threshold for significance [66]. (v) KO mice, to query the mouse phenotype, BioMart was used to convert the human gene ensemble IDs to mouse gene ensemble IDs [67]. The Mammalian Phenotype Ontology Browser, which includes information on mice and other mammalian phenotypes, was used as a data source. The gene set was considered significant when the FDR in the enrichment analysis was <0.05.
According to our functional annotations, genes with one functional annotation were assigned 1 point, and genes with a score of ≥2 points were identified as biological depression risk genes.

2.4. STRING and DrugBank Analysis

The STRING database provides information related to gene-encoded proteins. The identified depression risk genes were subjected to STRING analysis according to the proteins that they encoded [68]. The proteins encoded by the identified genes were considered potential drug targets, and were subjected to further analysis conducted using DrugBank, a large database (https://www.drugbank.ca/, accessed on 17 February 2022) with data for over 17,000 drug targets and 10,000 drug compounds [69].

2.5. Validation of Target Genes for Depression

The drugs identified from DrugBank were confirmed through two databases: ClincalTrial.gov (https://clinicaltrials.gov/, accessed on 19 February 2022) was used for the drugs undergoing human trials, and PubMed (https://pubmed.ncbi.nlm.nih.gov/, accessed on 19 February 2022) was used for the drugs undergoing preclinical (in vitro and in vivo) studies.

3. Results

We identified 5885 SNPs associated with depression (Supplementary Table S1), 632 of which were unique. The genes with the identified SNPs were identified as depression-associated genes (Supplementary Table S2).

3.1. Depression Risk Genes Identified Using Functional Annotations

We assigned each of the 632 unique depression-associated genes a score according to their functional annotations. The distribution of the functional annotations is illustrated in Figure 2. We used the missense variant and cis-eQTL annotations as the first and second criteria, respectively, for identifying and prioritizing the depression-associated genes. Overall, 34 and 68 of the depression-associated genes had missense and cis-eQTL SNPs, respectively. The third set of criteria for consideration, in terms of a depression-associated gene, were the gene ontology annotations. We identified 87 depression-associated genes. The fourth set of criteria were the PPI annotations. We identified 59 genes that overlapped with the depression-associated genes. The fifth set of criteria, the KEGG annotations, were used to perform an enrichment analysis on the molecular pathways. Sixteen depression-associated genes were identified in the KEGG-annotated pathways according to the enrichment analysis.
We compiled the scores of each of the genes (from 0 to 4 points) according to their functional annotations (Figure 3). The largest proportion of the genes (460 genes) had scores of 0 points. A total of 65 genes had scores ≥2, and were thus identified as depression risk genes (Table 1). Only seven of the genes—Interleukin 4 (IL4), Interleukin 18 Receptor 1 (IL18R1), Interleukin 6 Receptor (IL6R), Signal Transducer and Activator of Transcription 6 (STAT6), SMAD Family Member 3 (SMAD3), Interleukin 13 (IL13), and Toll-Like Receptor 1 (TLR1)—had scores of 4 points.

3.2. STRING Database for Gene Set Expansion

The STRING database, which combines publicly available data on direct (physical) and indirect (functional) protein–protein interactions, was used to extend the gene set of the 65 depression risk genes. Fifty interactions were selected from the database, and ultimately, 115 genes were selected as target genes and used in subsequent analyses (Supplementary Table S3).

3.3. Prioritization of Drug Repurposing Candidates for Depression

The DrugBank database was used to identify the druggable genes from among the 115 genes identified in the STRING analysis. Unfortunately, not all of the depression risk genes were druggable; only 19 of the genes were identified as druggable, and were determined as able to bind with 58 drugs. Of the seven genes with a score of 4 points, only IL6R was determined as druggable. All the identified target genes and drugs are listed in Supplementary Table S4.
Intriguingly, of the 58 identified drugs, 20 were undergoing clinical trials or preclinical studies for depression (Table 2). The other 38 drugs were new drugs that had never been previously reported as being used for the treatment of depression.
The target genes were those reported in preclinical studies and clinical trial studies to be the most promising target genes for depression. We identified nine target genes, including CD3 delta subunit of the T-cell receptor complex (CD3D), CD247 molecule (CD247), adenosine A1 receptor (ADORA1), cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), protein kinase C epsilon (PRKCE), ferritin light chain (FTL), interleukin 5 (IL5), gamma aminobutyric acid type B receptor subunit 1 (GABBR1), and IL6R. Of the 38 new drugs, the following 15 targeted six of the most promising target genes: sodium ferric gluconate complex, ferric pyrophosphate citrate, blinatumomab, reslizumab, sarilumab, satralizumab, aminophylline, oxtriphylline, metocurine iodide, doxacurium, tubocurarine, decamethonium, metocurine, pancuronium, and pipecuronium (Table 3). Of these, we highlight sarilumab and satralizumab as exhibiting the most potential as drug repurposing candidates for depression because they target IL6R, which was identified as the gene exhibiting the strongest potential as a target gene according to the functional annotation scoring system and the validation conducted using the ClinicalTrials.gov and PubMed databases (Table 3).

4. Discussion

This study integrated machine learning and functional annotations to identify drug repurposing candidates for the treatment of depression. We identified seven key depression risk genes according to their highest functional annotation scores, and identified IL6R as the most promising target gene for depression according to clinical and preclinical evidence. In addition, we identified approximately 20 drugs undergoing clinical trials and preclinical studies for use in the treatment of depression, and 15 new drug repurposing candidates, including sarilumab and satralizumab, exhibiting strong potential for use in the treatment of depression. These findings indicate that adopting a genomic-analysis-based approach to drug repurposing can facilitate the discovery of new drugs for treating depression.
IL6R was one of the target genes with the highest functional annotation score and was a highly promising target in the treatment of depression. IL6R regulates systemic inflammation, which is associated with depression development [70,71,72]. Genetic variants of IL6R are associated with interleukin 6 (IL6) and C-reactive protein (CRP) regulation [73]. Furthermore, the upregulation and downregulation of IL6 and CRP affect depression severity [70,73]. Two major signaling pathways of IL6, classical signaling (anti-inflammatory) and trans-signaling (pro-inflammatory), were assumed to be related to depression development [74]. The classical signaling pathway occurs when IL6 binds with IL6R (a membrane-bound receptor) [75,76], while the trans-signaling pathway involves the attachment of IL6 to soluble interleukin 6 receptor (sIL6R), a non-membrane-bound receptor [76,77]. The activity of IL6 in the brain was often induced by trans-signaling [78,79,80,81]. A recent Mendelian randomization study showed that an increased number of sIL6Rs in the trans-signaling pathway significantly enhances the risk of depression [82]. In addition, high levels of sIL6R are associated with lower CRP production through classical signaling, which indicates a high risk of depression [82]. Tocilizumab, which is undergoing clinical studies under accession number NCT03787290, is a humanized monoclonal antibody that targets IL6R, thereby inhibiting IL6 classic signaling and trans-signaling pathways [83], and is effective at alleviating depressive symptoms [84]. An interventional study reported the benefit of tocilizumab in decreasing depression severity [85]. In addition, we identified two other drugs that target IL6R: sarilumab and satralizumab. Although no evidence regarding the use of these two drugs in the treatment of depression has yet been uncovered, they exhibit strong potential as drug repurposing candidates for depression.
IL5 encodes a cytokine that is an effector cytokine of activated Th2 cells; that is, IL5 activates Th cells after the cells are activated by IL4 [86]. Depression was associated with a lower IFN-γ level and an elevated IL13 level; the functions of IL13 have similarities with the role of IL5 [87]. Thus, IL5 might be associated with depression. This was supported by a gene set analysis study, in which IL5 was upregulated in the post-mortem brain tissue of a patient with MDD [88]. A study that investigated the association between IL5 and MDD in 116 participants (MDD = 58; control = 58) revealed that every 1-unit increase in serum IL5 level is associated with a 76% greater risk of MDD [64]. In addition, a study by Tzang et al. observed that IL5 level is associated with depression symptoms in cancer patients [89]. A possible mechanism has been proposed that increased amounts of cortisol in the circulation cause aberrant IL-5 cytokine production and secretion patterns, which in turn cause depressed symptoms [90]. Mepolizumab is a fully humanized recombinant IgG1 kappa monoclonal antibody against IL5, and has been approved for severe asthma [91]. In a previous study, mepolizumab administered for 6 months significantly reduced the occurrence of asthma exacerbations (from 48% to 38%) in patients with asthma and comorbid depression [92]. Mepolizumab is undergoing clinical trials for depression in patients with asthma (accession number: NCT-04680611). Another drug candidate identified in the present study is reslizumab, which targets IL5. We suggest that the mechanisms underlying the effect of reslizumab on the pathophysiology of depression involve IL5.
CHRNA2 is a widely expressed subunit of nicotinic acetylcholine receptors, and is involved in neurocognitive disorders and nicotine dependence [93,94,95,96]. The position of CHRNA2 in chromosomes (in the 8p region) may be involved in neurodegenerative and psychiatric disorders [96]. A study on prenatal depression patients found that differentially methylated CHRNA2 related to antidepressant treatment [97]. In other words, CHRNA2 was considered to be involved in depression pathogenesis. In the present study, carbamoylcholine, cisatracurium, atracurium besylate, mivacurium, vecuronium, and two drugs of which the clinical efficacy was confirmed through clinical trials (mecamylamine and ruconium) were determined to target CHRNA2. The non-competitive antagonist mecamylamine, a widely used therapeutic agent that targets acetylcholine receptors, may be effective in depression treatment [98]. In addition, reconium, originally used as a muscle relaxant, may have antidepressant effects, and is an effective adjunctive treatment with electroconvulsive therapy (ECT) [99,100]. Rocunium has also been observed to reduce myalgia and headache, and shorten the awakening time (spontaneous respiration and opening the eyes in response to verbal stimuli) after ECT [100].
Another target gene that we identified in the present study was ADORA1, which regulates various biological functions, including the mechanisms underlying sleep [101,102], and psychiatric disorders including depression [103,104,105]. ADORA1 activation has been found to induce antidepressant-like effects [106,107]. In addition, the therapeutic effects of sleep deprivation [108] and ECT [109] are mediated by the activation or upregulation of ADORA1. Szopa et al. suggested a new approach in the treatment of people with depressive disorders that involves combining selective A1 and A2A receptor antagonists with magnesium or zinc [110]. Tramadol, a drug undergoing phase IV clinical trials for depression, was discovered to target ADORA1 in the present study. Bumpus [111] assessed patients’ perceptions of the effectiveness and safety of tramadol as an off-label antidepressant relative to 34 other antidepressants, and discovered that most (94.6%) of the patients viewed tramadol as an effective antidepressant. Tramadol is a mu-receptor opioid agonist that increases the concentrations of serotonin and noradrenaline in the limbic system, thereby exerting an antidepressant effect [112]. In addition to tramadol, we identified other drugs linked to ADORA1, including caffeine, theophylline, adenosine, and pentoxifylline, that were undergoing phase 1 and 2 clinical trials. Furthermore, we discovered other target genes and drug repurposing candidates for depression, the efficacies of which are supported by published evidence; for example, muromanab, which targets CD3D/CD247 [113,114], and taurine, which targets GABBR1 [115,116].
In addition, in terms of neuroinflammation, STAT6 was found to be associated with neurodegeneration diseases, including depression [117,118]. Interestingly, STAT6 exhibited one of the highest scores based on the five functional annotations in the present study. Several previous studies support the role of STAT6 in depression; these were validated in a preclinical investigation, in which STAT6 signaling was discovered to be involved in some of the brain’s mechanisms, such as the activity of neurons and neuroplasticity [119,120]. Previous studies using animal models emphasized that a deficiency of STAT6 decreases levels of dopamine and serotonin transporter; thus, STAT6 is suggested to play a pivotal role in the pathogenesis of depression through monoamine regulation in the hippocampus of the brain [119,121]. To date, this result has not been confirmed in clinical studies. Unfortunately, the drug target genes that we identified are not all involved in pharmacological activities (undruggable), including STAT6. However, we propose that STAT6 can be considered as a potential biomarker for depression.
Drug repurposing offers several advantages, such as a shorter time period, being cost effective, and imposing less risk compared to traditional drug discovery [122]. Additionally, in this study, drug repurposing by genomic analysis presents the strength of its ability to bridge the gap between genomic medicine and conventional personalized trials for the treatment of depression by offering new perspectives on pharmacogenomic-guided medication based on biological depression risk genes of depression patients. Despite the fact that our study demonstrates the feasibility and value of using SNP data to determine drug repurposing candidates for the treatment of depression, it still has some limitations. Not all SNPs are biologically significant, and not all the identified depression risk genes could be targeted by drugs. Moreover, due to the nature of present study, we were unable to investigate the therapeutic effects of our findings. For future research, more functional in vitro and in vivo investigations (in primary basic/preclinical research or clinical trials) and validations are required.

5. Conclusions

In this study, we reveal a schematic approach for the use of functional genomic variations to identify drug target genes and potential repurposed drugs prior to preclinical and clinical trial studies for depression. Our findings propose IL6R as the most promising target gene for depression due to IL6R exhibiting the highest functional annotation score and its validation in ClinicalTrial.gov and PubMed databases. In addition, we identified two candidate drugs (sarilumab and satralizumab) with strong potential use in the treatment of depression. In summary, this study indicates that using a genomic-analysis-based approach to discover drugs for treating depression is both time- and cost-effective. Furthermore, the findings of our study can serve as evidence of genes related to the inflammatory pathway, and provide new insight into pathomechanism for depression. Future studies need to investigate the role of IL6R in the pathogenesis of depression, as well as the interactions between IL6R and sarilumab or satralizumab.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/biomedicines10081947/s1, Table S1: SNP prioritization; Table S2: Scoring of five functional annotation; Table S3: Protein annotation STRING; Table S4: Druggable biogenes; Table S5: PubMed drug search.

Author Contributions

Conceptualized: M.H.S.L. and M.-H.C. Data curation: N.Q.K.L., K.-H.C., W.-C.C. and C.-Y.W. Data analysis: M.H.S.L. and L.M.I. Data interpretation: M.H.S.L., L.M.I. and M.-H.C. Original manuscript writing: M.H.S.L. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by Cathay General Hospital (107CGH-TMU-06) and Ministry of Education, Taiwan (104-R-0005).

Institutional Review Board Statement

This study was approved by Taipei Medical University-Joint Institutional Review Board (No. N201807007 and No. 201506009).

Informed Consent Statement

Informed consent was obtained from all subjects involved in the study.

Data Availability Statement

The data presented in this study are available on request from the corresponding author. The data are not publicly available due to privacy.

Acknowledgments

We acknowledge the hospital administration for their assistance and Ismaila Sonko for English Editing.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. Overview of drug repurposing for depression. In this study design, SNPs were prioritized using a machine learning algorithm and various databases: HaploReg v4.1, STRING, DrugBank, ClinicalTrials.gov, and PubMed.
Figure 1. Overview of drug repurposing for depression. In this study design, SNPs were prioritized using a machine learning algorithm and various databases: HaploReg v4.1, STRING, DrugBank, ClinicalTrials.gov, and PubMed.
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Biomedicines 10 01947 g002 550
Figure 2. Histogram distribution of functional annotations.
Figure 2. Histogram distribution of functional annotations.
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Figure 3. Histogram distribution of gene scores: 460 and 111 genes had scores of 0 and 1, respectively; the 65 genes with total scores ≥2 were identified as “depression risk genes”.
Figure 3. Histogram distribution of gene scores: 460 and 111 genes had scores of 0 and 1, respectively; the 65 genes with total scores ≥2 were identified as “depression risk genes”.
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Table
Table 1. Five functional annotations applied to prioritize the depression risk genes.
Table 1. Five functional annotations applied to prioritize the depression risk genes.
GENCODE_IDGENCODE_NameMissense VariantCis-eQTLKEGGPPIKO MiceTotal Score
ENSG00000113520IL4011114
ENSG00000115604IL18R1011114
ENSG00000160712IL6R110114
ENSG00000166888STAT6011114
ENS G00000166949SMAD3011114
ENSG00000169194IL13101114
ENSG00000174125TLR1110114
ENSG00000020633RUNX3010113
ENSG00000069667RORA001113
ENSG00000107485GATA3001113
ENSG00000109471IL2001113
ENSG00000113525IL5001113
ENSG00000115602IL1RL1100113
ENSG00000117586TNFSF4010113
ENSG00000125347IRF1010113
ENSG00000134215VAV3010113
ENSG00000138684IL21001113
ENSG00000141736ERBB2100113
ENSG00000158869FCER1G010113
ENSG00000161405IKZF3010113
ENSG00000179344HLA-DQB1011013
ENSG00000204252HLA-DOA001113
ENSG00000204287HLA-DRA111003
ENSG00000231389HLA-DPA1011103
ENSG00000073605GSDMB110002
ENSG00000074047GLI2000112
ENSG00000079112CDH17000112
ENSG00000087086FTL000102
ENSG00000087088BAX000112
ENSG00000100385IL2RB010012
ENSG00000100902PSMA6010002
ENSG00000106571GLI3000112
ENSG00000107957SH3PXD2A100012
ENSG00000111145ELK3100 12
ENSG00000111335OAS2110 02
ENSG00000112130RNF8000112
ENSG00000112486CCR6000112
ENSG00000113522RAD50000012
ENSG00000120903CHRNA2010012
ENSG00000124107SLPI000112
ENSG00000131507NDFIP1000112
ENSG00000134460IL2RA000112
ENSG00000134470IL15RA010012
ENSG00000135905DOCK10000112
ENSG00000137033IL33000112
ENSG00000142556ZNF614110002
ENSG00000143631FLG100012
ENSG00000145777TSLP000112
ENSG00000162104ADCY9010012
ENSG00000163485ADORA1010012
ENSG00000165280VCP000112
ENSG00000167914GSDMA110002
ENSG00000171132PRKCE000112
ENSG00000171608PIK3CD000112
ENSG00000172057ORMDL3010102
ENSG00000174130TLR6000112
ENSG00000179588ZFPM1000112
ENSG00000180902D2HGDH110002
ENSG00000186265BTLA000112
ENSG00000186716BCR010102
ENSG00000196735HLA-DQA1011002
ENSG00000197746PSAP000112
ENSG00000198821CD247010012
ENSG00000204681GABBR1100012
ENSG00000215182MUC5AC100012
Note: The italic font in table indicates genes name; the colors of missense variant: orange, cis-eQTL: green, KEGG: red, PPI: blue, KO mice: purple meaning scored as 1; The darker of grey color in total score meaning higher scores.
Table
Table 2. Pharmacological therapies in development for the treatment of depression.
Table 2. Pharmacological therapies in development for the treatment of depression.
GeneDrugOriginal IndicationIdentifier * (NCT-0/PMID)
ClinicalTrials.gov
FTLIron DextranIron deficiency3373253
IL5MepolizumabEosinophilic granulomatosis with polyangiitis (EGPA)4680611
IL6RTocilizumabRheumatoid arthritis3787290
ADORA1TramadolModerate to severe pain3309163
ADORA1CaffeineMigraine0025792
ADORA1TheophyllineChronic asthma1263106
ADORA1AdenosineTachycardia2902601
ADORA1PentoxifyllineIntermittent claudication4417049
PRKCETamoxifenBreast cancer0667121
CHRNA2MecamylamineHypertension0593879
CHRNA2RocuroniumGeneral anesthesia4565730
GABBR1TaurineTotal parenteral nutrition0217165
PubMed
CD3DMuromonabPrevention of organ rejection24257035
CD247MuromonabPrevention of organ rejection24257035
ADORA1DyphyllineAsthma10064181
CHRNA2CarbamoylcholineOpen-angle glaucoma23603524
CHRNA2CisatracuriumGeneral anesthesia22092267
CHRNA2Atracurium besylateGeneral anesthesia8442962
CHRNA2MivacuriumGeneral anesthesia8346843
CHRNA2VecuroniumMuscle relaxant8733812
Note: * Identifiers from ClinicalTrials.gov and PubMed database; the italic font indicates genes name.
Table
Table 3. Drug repurposing candidates for depression identified using a genomic-analysis-based approach.
Table 3. Drug repurposing candidates for depression identified using a genomic-analysis-based approach.
Biological GeneTarget DrugOriginal IndicationScore
IL6RSarilumabRheumatoid arthritis4
IL6RSatralizumabNeuromyelitis optica spectrum disorder (NMOSD)4
IL5ReslizumabSevere asthma3
sFTLSodium ferric gluconate complexIron deficiency anemia2
FTLFerric pyrophosphate citrateIron deficiency2
CD3DBlinatumomabAcute lymphoblastic leukemia (ALL)2
ADORA1AminophyllineAsthma2
ADORA1OxtriphyllineAsthma2
CHRNA2Metocurine iodideMuscle contractions2
CHRNA2DoxacuriumGeneral anesthesia2
CHRNA2TubocurarineGeneral anesthesia2
CHRNA2DecamethoniumMuscle relaxant2
CHRNA2MetocurineMuscle relaxant2
CHRNA2PancuroniumMuscle relaxant2
CHRNA2PipecuroniumMuscle relaxant2
Note: Scores were obtained from a scoring system based on five sets of functional annotations; the italic font indicates genes name.
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Lesmana, M.H.S.; Le, N.Q.K.; Chiu, W.-C.; Chung, K.-H.; Wang, C.-Y.; Irham, L.M.; Chung, M.-H. Genomic-Analysis-Oriented Drug Repurposing in the Search for Novel Antidepressants. Biomedicines 2022, 10, 1947. https://doi.org/10.3390/biomedicines10081947

AMA Style

Lesmana MHS, Le NQK, Chiu W-C, Chung K-H, Wang C-Y, Irham LM, Chung M-H. Genomic-Analysis-Oriented Drug Repurposing in the Search for Novel Antidepressants. Biomedicines. 2022; 10(8):1947. https://doi.org/10.3390/biomedicines10081947

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Lesmana, Mohammad Hendra Setia, Nguyen Quoc Khanh Le, Wei-Che Chiu, Kuo-Hsuan Chung, Chih-Yang Wang, Lalu Muhammad Irham, and Min-Huey Chung. 2022. "Genomic-Analysis-Oriented Drug Repurposing in the Search for Novel Antidepressants" Biomedicines 10, no. 8: 1947. https://doi.org/10.3390/biomedicines10081947

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