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Biomedicines
Volume 11
Issue 3
10.3390/biomedicines11030863
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Article

Bag-1 Protects Nucleus Pulposus Cells from Oxidative Stress by Interacting with HSP70

by
Kaori Suyama
1,*,
Daisuke Sakai
2,
Shogo Hayashi
1,
Ning Qu
1,
Hayato Terayama
1,
Daisuke Kiyoshima
1,
Kenta Nagahori
1 and
Masahiko Watanabe
2
1
Department of Anatomy and Cellular Biology, Basic Medical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara 259-1193, Kanagawa, Japan
2
Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara 259-1193, Kanagawa, Japan
*
Author to whom correspondence should be addressed.
Biomedicines 2023, 11(3), 863; https://doi.org/10.3390/biomedicines11030863
Submission received: 18 February 2023 / Revised: 8 March 2023 / Accepted: 9 March 2023 / Published: 12 March 2023
(This article belongs to the Section Cell Biology and Pathology)
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Abstract

Bcl-2-associated athanogene 1 (Bag-1) is a multifunctional prosurvival protein that binds to several intracellular targets and promotes cell survival. HSP70 and Raf-1 are important targets of Bag-1; however, the protective function of Bag-1 in nucleus pulposus (NP) cells remains unclear. In this study, we determined the effects of Bag-1 on NP cells under oxidative stress induced by treatment with hydrogen peroxide (H2O2). We found that Bag-1 was bound to HSP70, but Bag-1–Raf1 binding did not occur in NP cells. Bag-1 overexpression in NP cells enhanced cell viability and mitochondrial function and significantly suppressed p38/MAPKs phosphorylation during oxidative stress, although NP cells treated with a Bag-1 C-terminal inhibitor, which is the binding site of HSP70 and Raf-1, decreased cell viability and mitochondrial function during oxidative stress. Furthermore, the phosphorylation of the ERK/MAPKs was significantly increased in Bag-1 C-terminal inhibitor-treated NP cells without H2O2 treatment but did not change with H2O2 exposure. The phosphorylation of Raf-1 was not influenced by Bag-1 overexpression or Bag-1 C-terminal binding site inhibition. Overall, the results suggest that Bag-1 preferentially interacts with HSP70, rather than Raf-1, to protect NP cells against oxidative stress.
Keywords: Bag-1; nucleus pulposus; oxidative stress; HSP70 Bag-1; nucleus pulposus; oxidative stress; HSP70

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MDPI and ACS Style

Suyama, K.; Sakai, D.; Hayashi, S.; Qu, N.; Terayama, H.; Kiyoshima, D.; Nagahori, K.; Watanabe, M. Bag-1 Protects Nucleus Pulposus Cells from Oxidative Stress by Interacting with HSP70. Biomedicines 2023, 11, 863. https://doi.org/10.3390/biomedicines11030863

AMA Style

Suyama K, Sakai D, Hayashi S, Qu N, Terayama H, Kiyoshima D, Nagahori K, Watanabe M. Bag-1 Protects Nucleus Pulposus Cells from Oxidative Stress by Interacting with HSP70. Biomedicines. 2023; 11(3):863. https://doi.org/10.3390/biomedicines11030863

Chicago/Turabian Style

Suyama, Kaori, Daisuke Sakai, Shogo Hayashi, Ning Qu, Hayato Terayama, Daisuke Kiyoshima, Kenta Nagahori, and Masahiko Watanabe. 2023. "Bag-1 Protects Nucleus Pulposus Cells from Oxidative Stress by Interacting with HSP70" Biomedicines 11, no. 3: 863. https://doi.org/10.3390/biomedicines11030863

APA Style

Suyama, K., Sakai, D., Hayashi, S., Qu, N., Terayama, H., Kiyoshima, D., Nagahori, K., & Watanabe, M. (2023). Bag-1 Protects Nucleus Pulposus Cells from Oxidative Stress by Interacting with HSP70. Biomedicines, 11(3), 863. https://doi.org/10.3390/biomedicines11030863

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