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Biomedicines
Volume 11
Issue 4
10.3390/biomedicines11041174
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Article
Peer-Review Record

Urine Dipstick Analysis on Automated Platforms: Is a Reliable Screening Tool for Proteinuria? An Experience from Umberto I Hospital in Rome

Biomedicines 2023, 11(4), 1174; https://doi.org/10.3390/biomedicines11041174
by Sergio Terracina 1,†, Antonio Pallaria 1,†, Marco Lucarelli 1, Antonio Angeloni 1, Annarita De Angelis 1, Flavio Maria Ceci 1, Brunella Caronti 2, Silvia Francati 1, Giovanna Blaconà 1, Marco Fiore 3,*,‡ and Giampiero Ferraguti 1,*,‡
Reviewer 1:
Thach N. Nguyen
Reviewer 2: Anonymous
Biomedicines 2023, 11(4), 1174; https://doi.org/10.3390/biomedicines11041174
Submission received: 17 March 2023 / Revised: 7 April 2023 / Accepted: 10 April 2023 / Published: 13 April 2023
(This article belongs to the Topic Proteomics and Metabolomics in Biomedicine)

Round 1

Reviewer 1 Report

This is a retrospective study of 249 patients. The aim was to verify the reliability of an automated urinalysis dipstick method comparing the results with the conventional quantitative test of creatinine and albumin performed on a clinical chemistry platform. The results showed a good correlation between the two assays, even the dipstick assessment tended to overestimate the Albumin creatinine ratio (ACR) with higher false positives. The study analyzed the results based on age (starting from pediatric to aged patients) and gender. The positive values needed to be confirmed with quantitative methods, especially in women and younger people. Patients with microalbuminuria (ACR 30-300mg/g) or with severe albumin urinary excretion (ACR>300mg/g) should be reanalyzed using quantitative methods to obtain a more reliable calculation of the ACR.

The methodology was great. The discussion was sufficient. The English writing was great

Author Response

We do thank the reviewer for the positive comments. As suggested, we performed a spellig check of the manuscript.

Reviewer 2 Report

Dear Authors, 

The research carried out in MS ´Urine dipstick analysis on automated platforms: is a reliable 2 screening tool for proteinuria? An experience from Umberto I 3 Hospital in Rome.´ is very important and significant to developing a quick testing kit for diagnostics. 

However, I have some difficulties understanding the MS. 

1- did you perform any surface modification or coating of material on dipsticks to enhance the adsorption of the protein of interest? 

2- it would be good if you can provide clear quantitative comparison data with other techniques and what percentage of similarity is achieved. 

3- what are the chances of false positives and negatives? 

4- there are some other similar products in kit available, can we compare it with any product which is in the market? 

5- A schematic diagram for sample preparation and test would also help readers to understand. 

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

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