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Biomedicines
Volume 11
Issue 5
10.3390/biomedicines11051238
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Review

Increasing Specificity of Targeted DNA Methylation Editing by Non-Enzymatic CRISPR/dCas9-Based Steric Hindrance

by
Daniel M. Sapozhnikov
and
Moshe Szyf
*
Department of Pharmacology and Therapeutics, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC H3G 1Y6, Canada
*
Author to whom correspondence should be addressed.
Biomedicines 2023, 11(5), 1238; https://doi.org/10.3390/biomedicines11051238
Submission received: 31 March 2023 / Revised: 19 April 2023 / Accepted: 20 April 2023 / Published: 22 April 2023
(This article belongs to the Special Issue Molecular Tools for Epigenetic Engineering)
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Abstract

As advances in genome engineering inch the technology towards wider clinical use—slowed by technical and ethical hurdles—a newer offshoot, termed “epigenome engineering”, offers the ability to correct disease-causing changes in the DNA without changing its sequence and, thus, without some of the unfavorable correlates of doing so. In this review, we note some of the shortcomings of epigenetic editing technology—specifically the risks involved in the introduction of epigenetic enzymes—and highlight an alternative epigenetic editing strategy using physical occlusion to modify epigenetic marks at target sites without a requirement for any epigenetic enzyme. This may prove to be a safer alternative for more specific epigenetic editing.
Keywords: epigenetic editing; DNA methylation; CRISPR/Cas9; dCas9; DNMT1; DNMT3A; TET1; steric hindrance; demethylation epigenetic editing; DNA methylation; CRISPR/Cas9; dCas9; DNMT1; DNMT3A; TET1; steric hindrance; demethylation

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MDPI and ACS Style

Sapozhnikov, D.M.; Szyf, M. Increasing Specificity of Targeted DNA Methylation Editing by Non-Enzymatic CRISPR/dCas9-Based Steric Hindrance. Biomedicines 2023, 11, 1238. https://doi.org/10.3390/biomedicines11051238

AMA Style

Sapozhnikov DM, Szyf M. Increasing Specificity of Targeted DNA Methylation Editing by Non-Enzymatic CRISPR/dCas9-Based Steric Hindrance. Biomedicines. 2023; 11(5):1238. https://doi.org/10.3390/biomedicines11051238

Chicago/Turabian Style

Sapozhnikov, Daniel M., and Moshe Szyf. 2023. "Increasing Specificity of Targeted DNA Methylation Editing by Non-Enzymatic CRISPR/dCas9-Based Steric Hindrance" Biomedicines 11, no. 5: 1238. https://doi.org/10.3390/biomedicines11051238

APA Style

Sapozhnikov, D. M., & Szyf, M. (2023). Increasing Specificity of Targeted DNA Methylation Editing by Non-Enzymatic CRISPR/dCas9-Based Steric Hindrance. Biomedicines, 11(5), 1238. https://doi.org/10.3390/biomedicines11051238

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