Spheroids of FAP-Positive Cell Lines as a Model for Screening Drugs That Affect FAP Expression

Pleshkan, Victor V.; Zinovyeva, Marina V.; Antonova, Dina V.; Alekseenko, Irina V.

doi:10.3390/biomedicines11072017

Open AccessArticle

Spheroids of FAP-Positive Cell Lines as a Model for Screening Drugs That Affect FAP Expression

¹

Gene Immunooncotherapy Group, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia

²

National Research Center “Kurchatov Institute”, 123182 Moscow, Russia

³

Laboratory of Epigenetics, Institute of Oncogynecology and Mammology, National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov, Ministry of Healthcare of the Russian Federation, 117198 Moscow, Russia

^*

Author to whom correspondence should be addressed.

Biomedicines 2023, 11(7), 2017; https://doi.org/10.3390/biomedicines11072017

Submission received: 10 June 2023 / Revised: 13 July 2023 / Accepted: 15 July 2023 / Published: 18 July 2023

(This article belongs to the Special Issue Tumor Microenvironment: From Cellular Components to Therapeutic Perspectives)

Download

Browse Figures

Versions Notes

Abstract

Fibroblast activation protein has a unique expression profile that manifests mainly in wounds and tumors, which anticipates it as an encouraging and selective target for anticancer therapy. However, research of the therapeutic potential of FAP is limited both by legal restraints when working in vivo and by the difficulty of obtaining standardized primary cultures of FAP-positive cancer-associated fibroblasts due to their high heterogeneity. We found that 3D spheroids of FAP-positive cell lines could serve as robust and convenient models of FAP expression, in contrast to monolayers. By exposing such spheroids to various factors and compounds, it is possible to study changes in FAP expression, which are easily detected by confocal microscopy. FAP expression increases under the influence of the TGFβ, does not depend on pH, and decreases during hypoxia and starvation. We believe that the proposed model could be used to organize large-scale high-throughput screening of drugs that target FAP expression.

Keywords: fibroblast activation protein; cancer-associated fibroblasts; 3D spheroids

Share and Cite

MDPI and ACS Style

Pleshkan, V.V.; Zinovyeva, M.V.; Antonova, D.V.; Alekseenko, I.V. Spheroids of FAP-Positive Cell Lines as a Model for Screening Drugs That Affect FAP Expression. Biomedicines 2023, 11, 2017. https://doi.org/10.3390/biomedicines11072017

AMA Style

Pleshkan VV, Zinovyeva MV, Antonova DV, Alekseenko IV. Spheroids of FAP-Positive Cell Lines as a Model for Screening Drugs That Affect FAP Expression. Biomedicines. 2023; 11(7):2017. https://doi.org/10.3390/biomedicines11072017

Chicago/Turabian Style

Pleshkan, Victor V., Marina V. Zinovyeva, Dina V. Antonova, and Irina V. Alekseenko. 2023. "Spheroids of FAP-Positive Cell Lines as a Model for Screening Drugs That Affect FAP Expression" Biomedicines 11, no. 7: 2017. https://doi.org/10.3390/biomedicines11072017

APA Style

Pleshkan, V. V., Zinovyeva, M. V., Antonova, D. V., & Alekseenko, I. V. (2023). Spheroids of FAP-Positive Cell Lines as a Model for Screening Drugs That Affect FAP Expression. Biomedicines, 11(7), 2017. https://doi.org/10.3390/biomedicines11072017

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

Spheroids of FAP-Positive Cell Lines as a Model for Screening Drugs That Affect FAP Expression

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI