Role of Syndecan-4 in the Inhibition of Articular Cartilage Degeneration in Osteoarthritis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Animal Models
2.2. Intra-Articular Injection of SDC4 in OA Model Mice
2.3. Analysis of SDC4 Injected into Knee Joints
2.4. Histopathological Assessment
2.5. Histopathological Evaluation
2.5.1. Histological Grading of Cartilage and the Synovial Membrane
2.5.2. Immunohistochemistry
2.6. Chondrocyte Isolation and Culture
2.7. RNA Extraction and cDNA Synthesis
2.8. Real-Time PCR
2.9. Statistical Analysis
3. Results
3.1. Animal Welfare
3.2. Distribution of SDC4 Injected into Knee Joints
3.3. Histological Analysis and Grading of OA Model Mice
3.4. Immunohistochemistrical Analysis
3.5. Gene Expression in Chondrocytes
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hattori, Y.; Hasegawa, M.; Iino, T.; Imanaka-Yoshida, K.; Sudo, A. Role of Syndecan-4 in the Inhibition of Articular Cartilage Degeneration in Osteoarthritis. Biomedicines 2023, 11, 2257. https://doi.org/10.3390/biomedicines11082257
Hattori Y, Hasegawa M, Iino T, Imanaka-Yoshida K, Sudo A. Role of Syndecan-4 in the Inhibition of Articular Cartilage Degeneration in Osteoarthritis. Biomedicines. 2023; 11(8):2257. https://doi.org/10.3390/biomedicines11082257
Chicago/Turabian StyleHattori, Yoshio, Masahiro Hasegawa, Takahiro Iino, Kyoko Imanaka-Yoshida, and Akihiro Sudo. 2023. "Role of Syndecan-4 in the Inhibition of Articular Cartilage Degeneration in Osteoarthritis" Biomedicines 11, no. 8: 2257. https://doi.org/10.3390/biomedicines11082257