Differential Regulation of Wingless-Wnt/c-Jun N-Terminal Kinase Crosstalk via Oxidative Eustress in Primary and Metastatic Colorectal Cancer Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Cultures and Treatments
2.2. Cell Viability and Metabolic Assay by MTS
2.3. Seahorse Assays
2.4. Gene Expression by Real-Time Quantitative PCR Analysis (qRT-PCR)
2.5. Protein Extraction and Western Blotting
2.6. Preparing Cell Blocks from SW480 and SW620 Cell Lines and Performing the Immunocytochemical Staining
2.7. Statistical Analysis
3. Results
3.1. Cell Viability after Oxidative Eustress Induced by H2O2 with and without JNK Inhibition
3.2. Oxygen Consumption Rate and Extracellular Acidification Rate Measurements
3.3. Gene Expression after Oxidative Stress Induced by H2O2 and JNK Inhibition
3.4. Protein Expression after H2O2 Eustress
3.5. Expression of Wnt Pathway Components in SW480 and SW620 Cell Block Sections
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Aceto, G.M.; Pagotto, S.; Del Pizzo, F.D.; Saoca, C.; Selvaggi, F.; Visone, R.; Cotellese, R.; Aguennouz, M.; Lattanzio, R.; Catalano, T. Differential Regulation of Wingless-Wnt/c-Jun N-Terminal Kinase Crosstalk via Oxidative Eustress in Primary and Metastatic Colorectal Cancer Cells. Biomedicines 2024, 12, 1816. https://doi.org/10.3390/biomedicines12081816
Aceto GM, Pagotto S, Del Pizzo FD, Saoca C, Selvaggi F, Visone R, Cotellese R, Aguennouz M, Lattanzio R, Catalano T. Differential Regulation of Wingless-Wnt/c-Jun N-Terminal Kinase Crosstalk via Oxidative Eustress in Primary and Metastatic Colorectal Cancer Cells. Biomedicines. 2024; 12(8):1816. https://doi.org/10.3390/biomedicines12081816
Chicago/Turabian StyleAceto, Gitana Maria, Sara Pagotto, Francesco Domenico Del Pizzo, Concetta Saoca, Federico Selvaggi, Rosa Visone, Roberto Cotellese, M’hammed Aguennouz, Rossano Lattanzio, and Teresa Catalano. 2024. "Differential Regulation of Wingless-Wnt/c-Jun N-Terminal Kinase Crosstalk via Oxidative Eustress in Primary and Metastatic Colorectal Cancer Cells" Biomedicines 12, no. 8: 1816. https://doi.org/10.3390/biomedicines12081816