An Artificial Intelligence-Based Digital Analysis of Immunolocalization of MMP2 and TIMP2 in the Jejunum of Rats Treated with Calcineurin Inhibitors
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Histochemical Analysis
2.3. Morphometric Analysis
2.4. Immunohistochemical Analysis
Artificial Intelligence (AI)-Related Technology
2.5. Statistical Analysis
3. Results
3.1. Histochemical Analysis
3.2. Morphometry
3.3. Immunohistochemistry—Digital Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Width of Submucosa | |||
---|---|---|---|
Control group | CMG | TMG | |
n = 6 | n = 6 | n = 6 | |
N | 30 | 30 | 30 |
AM ± SD | 42.95 ± 8.89 | 134.72 ± 20.94 * vs. control | 51.87 ± 9.38 * vs. control vs. CMG |
Med. | 43.45 | 139.3 | 51.5 |
CV | 20.71 | 15.54 | 18.09 |
Hight of Enterocytes | |||
Control group | CMG | TMG | |
n = 6 | n = 6 | n = 6 | |
N | 60 | 60 | 60 |
AM ± SD | 20.17 ± 2.056 | 17.19 ± 1.93 * vs. control, vs. TMG | 19.52 ± 2.24 * vs. CMG |
Med. | 19.89 | 17.07 | 19.67 |
CV | 10.21 | 11.23 | 11.49 |
Control | CMG | TMG | |
---|---|---|---|
MMP-2 | |||
Area | |||
N | 804 | 1285 | 765 |
AM ± SD | 311.50 ± 5813.075 | 346.88 ± 4779.96 * vs. control | 362.63 ± 642.18 * vs. control, CMG |
Med. | 13.94 | 3.44 | 9.004 |
CV | 1866.11 | 1377.95 | 1770.44 |
Perimeter | |||
N | 804 | 1285 | 765 |
AM ± SD | 75.36 ± 396.506 | 47.46 ± 360.61 * vs. control | 70.81 ± 463.68 * vs. control, CMG |
Med. | 20.95 | 11.55 | 17.07 |
CV | 526.14 | 759.71 | 654.746 |
TIMP-2 | |||
Area | |||
N | 733 | 456 | 86 |
AM ± SD | 333.98 ± 6015.93 | 749.34 ± 1468.91 * vs. control | 1693.01 ± 1512 * vs. control, CMG |
Med. | 16.06 | 7.12 | 1.36 |
CV | 1801.76 | 1950.75 | 893.21 |
Perimeter | |||
N | 733 | 456 | 86 |
AM ± SD | 77.69 ± 364.39 | 60.85 ± 539.32 * vs. control | 92.63 ± 611.87 * vs. control, CMG |
Med. | 24.102 | 15.43 | 5.802 |
CV | 469.02 | 886.21 | 660.49 |
Control | CMG | TMG | |
---|---|---|---|
Area | |||
MMP-2 (%) | 311.50 | 346.88 | 362.63 |
TIMP-2 (%) | 333.98 | 749.34 | 1693.01 |
TIMP-2-MMP-2 (%) | 22.48 | 402.46 | 1330.38 |
Perimeter | |||
MMP-2 (%) | 75.36 | 47.46 | 70.81 |
TIMP-2 (%) | 77.69 | 60.85 | 92.63 |
TIMP-2-MMP-2 (%) | 2.33 | 13.39 | 21.82 |
Control | CMG | TMG | ||||
---|---|---|---|---|---|---|
MMP2 | TIMP2 | MMP2 | TIMP2 | MMP2 | TIMP2 | |
(%) | (%) | (%) | (%) | (%) | (%) | |
0 | 83.13 | 100 | 5.45 | 100 | 0 | 2.61 |
1+ | 6.98 | 0 | 20.91 | 0 | 0 | 26.8 |
2+ | 9.88 | 0 | 67.27 | 0 | 40.5 | 66.01 |
3+ | 0 | 0 | 6.36 | 0 | 59.5 | 4.58 |
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Wilk, A.; Szumilas, K.; Gimpel, A.; Pilutin, A.; Rzeszotek, S.; Kędzierska-Kapuza, K. An Artificial Intelligence-Based Digital Analysis of Immunolocalization of MMP2 and TIMP2 in the Jejunum of Rats Treated with Calcineurin Inhibitors. Biomedicines 2024, 12, 1966. https://doi.org/10.3390/biomedicines12091966
Wilk A, Szumilas K, Gimpel A, Pilutin A, Rzeszotek S, Kędzierska-Kapuza K. An Artificial Intelligence-Based Digital Analysis of Immunolocalization of MMP2 and TIMP2 in the Jejunum of Rats Treated with Calcineurin Inhibitors. Biomedicines. 2024; 12(9):1966. https://doi.org/10.3390/biomedicines12091966
Chicago/Turabian StyleWilk, Aleksandra, Kamila Szumilas, Anna Gimpel, Anna Pilutin, Sylwia Rzeszotek, and Karolina Kędzierska-Kapuza. 2024. "An Artificial Intelligence-Based Digital Analysis of Immunolocalization of MMP2 and TIMP2 in the Jejunum of Rats Treated with Calcineurin Inhibitors" Biomedicines 12, no. 9: 1966. https://doi.org/10.3390/biomedicines12091966