Effects of Polyunsaturated Fatty Acid/Saturated Fatty Acid Ratio and Different Amounts of Monounsaturated Fatty Acids on Adipogenesis in 3T3-L1 Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Cell Culture
- (a)
- P/S ratio = 5; MUFA 60% was prepared by using the volume ratio of palmitic acid: oleic acid: linoleic acid equals 1:10:5;
- (b)
- P/S ratio = 5; MUFA 30% was prepared by using the volume ratio of palmitic acid: oleic acid: linoleic acid equals 1:2.5:5.
2.2. Cell Viability Assay
2.3. Cellular Fatty Acid Composition
2.4. Oil Red O Staining
2.5. Lipolysis Assay
2.6. RNA Isolation and Gene Expression Quantification
2.7. Apoptosis Assay
2.8. Statistical Analysis
3. Results
3.1. Effect of Different Concentrations of M60 and M30 Fatty Acid Composition on Adipocyte Viability
3.2. Effect of Different Concentrations of M60 and M30 Fatty Acid Compositions on Triglyceride Accumulation
3.3. Effect of Different Concentrations of M60 and M30 Fatty Acid Compositions on Adipocyte Lipolysis
3.4. Gene Expression of Adipogenic Enzymes
3.5. Gene Expression of Lipolytic Enzymes
3.6. Gene Expression of Adipokines Secreted by the Adipocytes
3.7. Fatty Acid Uptake of Every Single Fatty Acid
3.8. Effect of Different Ratios and Concentrations of Fatty Acids on Apoptosis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lim, S.Y.; Chien, Y.-W. Effects of Polyunsaturated Fatty Acid/Saturated Fatty Acid Ratio and Different Amounts of Monounsaturated Fatty Acids on Adipogenesis in 3T3-L1 Cells. Biomedicines 2024, 12, 1980. https://doi.org/10.3390/biomedicines12091980
Lim SY, Chien Y-W. Effects of Polyunsaturated Fatty Acid/Saturated Fatty Acid Ratio and Different Amounts of Monounsaturated Fatty Acids on Adipogenesis in 3T3-L1 Cells. Biomedicines. 2024; 12(9):1980. https://doi.org/10.3390/biomedicines12091980
Chicago/Turabian StyleLim, Sim Yee, and Yi-Wen Chien. 2024. "Effects of Polyunsaturated Fatty Acid/Saturated Fatty Acid Ratio and Different Amounts of Monounsaturated Fatty Acids on Adipogenesis in 3T3-L1 Cells" Biomedicines 12, no. 9: 1980. https://doi.org/10.3390/biomedicines12091980