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10.3390/biomedicines13030565
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Article

Nestin as a Marker Beyond Angiogenesis—Expression Pattern in Haemangiomas and Lymphangiomas

by
Andreas Mamilos
1,2,3,
Lina Winter
4,
Christoph B. Wiedenroth
5,
Tanja Niedermair
1,
Stefanie Zimmer
6,
Volker H. Schmitt
7,8,
Karsten Keller
7,9,10,
Ondrej Topolčan
11,
Marie Karlíková
11,
Markus Rupp
12,
Christoph Brochhausen
4,* and
Cristina Cotarelo
4
1
Institute of Pathology, University Regensburg, 93053 Regensburg, Germany
2
Department of Pathology, German Oncology Centre, 4108 Limassol, Cyprus
3
Medical Faculty, European University of Cyprus, 2404 Nicosia, Cyprus
4
Institute of Pathology, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany
5
Department of Thoracic Surgery, Kerckhoff Klinik, 61231 Bad Nauheim, Germany
6
Institute of Pathology and Tissue Bank, University Medical Center Mainz, 55131 Mainz, Germany
7
Department of Cardiology, University Medical Center of the Johannes Gutenberg-University Mainz, 55131 Mainz, Germany
8
German Center for Cardiovascular Research (DZHK), Partner Site Rhine Main, 55131 Mainz, Germany
9
Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University Mainz, 55131 Mainz, Germany
10
Medical Clinic VII, Department of Sports Medicine, University Hospital Heidelberg, 69120 Heidelberg, Germany
11
Central Laboratory for Immunoanalysis, Faculty of Medicine, Pilsen Charles University, 323 00 Pilsen, Czech Republic
12
Department for Trauma Surgery, University Hospital Regensburg, 93053 Regensburg, Germany
 
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Author to whom correspondence should be addressed.
Biomedicines 2025, 13(3), 565; https://doi.org/10.3390/biomedicines13030565
Submission received: 10 January 2025 / Revised: 14 February 2025 / Accepted: 21 February 2025 / Published: 24 February 2025
(This article belongs to the Special Issue Angiogenesis and Related Disorders)
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Abstract

Background: The intermediate filament nestin was first described in stem and progenitor cells of neural and mesenchymal origin. Additionally, it is expressed in endothelial cells during wound healing and tumorigenesis. Thus, nestin is widely regarded as a marker for proliferative endothelium. However, little is known about its role in lymphatic endothelium. Methods: Here, we analyzed the expression of nestin in the endothelium of ten human haemangiomas and ten lymphangiomas in situ by immunohistochemistry. This study aimed to investigate the expression of nestin in haemangiomas and lymphangiomas to determine its potential role as a vascular marker. Specifically, we aimed to assess whether nestin expression is restricted to proliferating endothelial cells or also present in non-proliferative blood vessels. Results: Immunohistochemically, haemangiomas were positive for CD31 but negative for D2-40. The endothelial cells within these lesions showed a homogeneous expression of nestin. In contrast, the endothelium of lymphangiomas reacted positively for D2-40 and CD31 but did not show any nestin expression. Additionally, only a few endothelial cells of capillary haemangiomas showed a Ki-67 positivity. Conclusions: The differential expression of nestin in haemangiomas and lymphangiomas indicates a specificity of nestin for the endothelium of blood vessels. The Ki-67 negativity in the majority of the endothelial cells reveals the proliferative quiescence of these cells. These findings indicate that nestin could be used as a marker to differentiate between blood and lymphatic vessels.
Keywords: nestin; haemangioma; lymphangioma; endothelial cells; immunohistochemistry; histology; CD31; D2-40 nestin; haemangioma; lymphangioma; endothelial cells; immunohistochemistry; histology; CD31; D2-40

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MDPI and ACS Style

Mamilos, A.; Winter, L.; Wiedenroth, C.B.; Niedermair, T.; Zimmer, S.; Schmitt, V.H.; Keller, K.; Topolčan, O.; Karlíková, M.; Rupp, M.; et al. Nestin as a Marker Beyond Angiogenesis—Expression Pattern in Haemangiomas and Lymphangiomas. Biomedicines 2025, 13, 565. https://doi.org/10.3390/biomedicines13030565

AMA Style

Mamilos A, Winter L, Wiedenroth CB, Niedermair T, Zimmer S, Schmitt VH, Keller K, Topolčan O, Karlíková M, Rupp M, et al. Nestin as a Marker Beyond Angiogenesis—Expression Pattern in Haemangiomas and Lymphangiomas. Biomedicines. 2025; 13(3):565. https://doi.org/10.3390/biomedicines13030565

Chicago/Turabian Style

Mamilos, Andreas, Lina Winter, Christoph B. Wiedenroth, Tanja Niedermair, Stefanie Zimmer, Volker H. Schmitt, Karsten Keller, Ondrej Topolčan, Marie Karlíková, Markus Rupp, and et al. 2025. "Nestin as a Marker Beyond Angiogenesis—Expression Pattern in Haemangiomas and Lymphangiomas" Biomedicines 13, no. 3: 565. https://doi.org/10.3390/biomedicines13030565

APA Style

Mamilos, A., Winter, L., Wiedenroth, C. B., Niedermair, T., Zimmer, S., Schmitt, V. H., Keller, K., Topolčan, O., Karlíková, M., Rupp, M., Brochhausen, C., & Cotarelo, C. (2025). Nestin as a Marker Beyond Angiogenesis—Expression Pattern in Haemangiomas and Lymphangiomas. Biomedicines, 13(3), 565. https://doi.org/10.3390/biomedicines13030565

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