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Biomedicines
Volume 9
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10.3390/biomedicines9010046
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Article
Peer-Review Record

Intestinal Adaptation upon Chemotherapy-Induced Intestinal Injury in Mice Depends on GLP-2 Receptor Activation

Biomedicines 2021, 9(1), 46; https://doi.org/10.3390/biomedicines9010046
by Anna Billeschou 1, Jenna Elizabeth Hunt 1,2, Aruna Ghimire 1, Jens J. Holst 1,2 and Hannelouise Kissow 1,2,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Biomedicines 2021, 9(1), 46; https://doi.org/10.3390/biomedicines9010046
Submission received: 18 November 2020 / Revised: 16 December 2020 / Accepted: 4 January 2021 / Published: 7 January 2021
(This article belongs to the Special Issue New Insights into Gut Hormones and Intestinal Diseases)

Round 1

Reviewer 1 Report

The paper by Billeschou describes small intestinal weight, crypt depth and villus height in Glp-2r deficient mice were not recovered after chemotherapy treatment. The authors have some novel data but some of the methods need to be clarified.

 

Major Comments:

  1. The authors just described the phenomenon. There are no data on mechanisms of Glp2r in intestinal adaptation. It is need to clear efficacy of Glp2r signaling.
  2. The elongation of crypt depth and villus height were seen in only duodenum. So Was the time point (at day 6 after 5-FU injection) sufficient to see these elongation?
  3. The authors didn’t elucidate what increasing of MPO activity in Glp2r deficient mice effect on intestinal adaptation.
  4. The authors used one marker for proliferative activity assay. How about Ki67 staining?

 

Mainor Comments:

  1. There are double words proliferative activity (under Fig. 3) and apoptosis (under Fig. 4).
  2. What does the last sentence of paragraph under Fig. 4 indicate? Maybe there are incorrect words or lack of words.
  3. “Decreased” in 8th sentence of 6th paragraph In discussion section is incorrect.

Comments for author File: Comments.docx

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Reviewer 2 Report

In the manuscript “Intestinal adaptation upon chemotherapy-induced intestinal injury in mice depends on GLP-2 receptor activation”, the authors investigated the role of GLP-2 receptor activation on intestinal protection and adaptation upon chemotherapy-induced intestinal injury; the injury was induced with a single injection of 5-fluorouracil in female GLP-2 receptor knockout (GLP-2R(-/-)) mice and their wild type (WT) littermates. The mice were euthanized in the acute or the recovery phase of the injury; the small intestines were analysed for weight changes, morphology, histology, inflammation, apoptosis and proliferation.

The manuscript is interesting and well written, and the results are clearly presented.

Author Response

Reviewer 2

 

We thank you very much for the time and effort to review our paper.

Yours Sincerely,

 Hannelouise Kissow

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