Inhibitory Effects of STAT3 Transcription Factor by Synthetic Decoy ODNs on Autophagy in Renal Fibrosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis of Decoy ODNs
2.2. Animal Model
2.3. Histologic Analysis
2.4. Immunohistochemical Staining
2.5. Immunofluorescent Staining
2.6. Western Blot Analysis
2.7. ELISA Assay
2.8. Blood Urea Nitrogen, and Creatinine Assays
2.9. Statistical Analysis
3. Results
3.1. Reduction in Renal Interstitial Injury and Fibrosis in UUO Mouse Kidneys Due to STAT3 Decoy ODNs
3.2. Inhibition of UUO-Induced Kidney Damage by STAT3 Decoy ODNs
3.3. Decrease in Autophagy Expression Due to STAT3 Decoy ODNs in UUO-Induced Renal Fibrosis
3.4. Regulation of Inflammatory Cytokines in the UUO-induced Renal Fibrosis Model by STAT3 Decoy ODNs
3.5. Regulation Effects of Autophagy by STAT3 Decoy ODNs
3.6. Induction of the PI3K-Akt-mTOR Pathway in a UUO Mouse Model with STAT3 Decoy ODN Injection
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Decoy | Sequence |
---|---|
Scr | 5′-GAATTCAATTCAGGGTACGGCAAAAAATTGCCGTACCCTGAATT-3′ |
STAT3 | 5′-GAATTCCCTTCCCGGAATTAAAAAATTCCCGGAAGG-3′ |
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Kim, Y.-A.; Kim, H.-J.; Gwon, M.-G.; Gu, H.; An, H.-J.; Bae, S.; Leem, J.; Jung, H.J.; Park, K.-K. Inhibitory Effects of STAT3 Transcription Factor by Synthetic Decoy ODNs on Autophagy in Renal Fibrosis. Biomedicines 2021, 9, 331. https://doi.org/10.3390/biomedicines9040331
Kim Y-A, Kim H-J, Gwon M-G, Gu H, An H-J, Bae S, Leem J, Jung HJ, Park K-K. Inhibitory Effects of STAT3 Transcription Factor by Synthetic Decoy ODNs on Autophagy in Renal Fibrosis. Biomedicines. 2021; 9(4):331. https://doi.org/10.3390/biomedicines9040331
Chicago/Turabian StyleKim, Young-Ah, Hyun-Ju Kim, Mi-Gyeong Gwon, Hyemin Gu, Hyun-Jin An, Seongjae Bae, Jaechan Leem, Hyun Jin Jung, and Kwan-Kyu Park. 2021. "Inhibitory Effects of STAT3 Transcription Factor by Synthetic Decoy ODNs on Autophagy in Renal Fibrosis" Biomedicines 9, no. 4: 331. https://doi.org/10.3390/biomedicines9040331