Type II Collagen-Conjugated Mesenchymal Stem Cells Micromass for Articular Tissue Targeting
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and Immunophenotype Analysis
2.2. Fabrication of Gelatin Microsphere
2.3. Cell Coating
2.4. Preparation of Cell Differentiation and Characterization
2.5. Evaluation of MSCs Differentiation
2.6. Immunofluorescence Staining
2.7. Measurement of Real-Time PCR
2.8. Sulfated Glycosaminoglycan (sGAG) Production Assay
2.9. Preliminary Assessment on Articular Cartilage Surface Binding
2.10. Statistical Analysis
3. Results
3.1. Stem Cell Characterization
3.2. Effect of Incubation Temperature and Period on Cell Glycosylation
3.3. Effect of PPG Coating on MSCs’ Proliferation and Differentiation in Monolayer Culture
3.4. Effect of PPG on MSCs’ Proliferation and Differentiation in 3D Culture
3.5. Conjugation of Antibody on PPG-MSCs in 3D Culture
3.6. Preliminary Assessment on Articular Cartilage Surface Attachment
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gen Name | Forward 5′-3′ | Reverse 5′-3′ |
---|---|---|
Type I Col | AAGGCTTCAAGGTCCCCCTGGTG | CAGCACCAGTAGCACCATCATTTC |
Type II Col | GGCAATAGCAGGTTCACGTACA | CGATAACAGTCTTGCCCCACTT |
Aggrecan | ACTTCCGCTGGTCAGATGGA | TCTCGTGCCAGATCATCACC |
GAPDH | GGCGATGCTGGCGCTGAGTAC | TGGTTCACACCCATGACGA |
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Sulaiman, S.B.; Chowdhury, S.R.; Busra, M.F.B.M.; Abdul Rani, R.B.; Mohamad Yahaya, N.H.B.; Tabata, Y.; Hiraoka, Y.; Haji Idrus, R.B.; Hwei, N.M. Type II Collagen-Conjugated Mesenchymal Stem Cells Micromass for Articular Tissue Targeting. Biomedicines 2021, 9, 880. https://doi.org/10.3390/biomedicines9080880
Sulaiman SB, Chowdhury SR, Busra MFBM, Abdul Rani RB, Mohamad Yahaya NHB, Tabata Y, Hiraoka Y, Haji Idrus RB, Hwei NM. Type II Collagen-Conjugated Mesenchymal Stem Cells Micromass for Articular Tissue Targeting. Biomedicines. 2021; 9(8):880. https://doi.org/10.3390/biomedicines9080880
Chicago/Turabian StyleSulaiman, Shamsul Bin, Shiplu Roy Chowdhury, Mohd Fauzi Bin Mh Busra, Rizal Bin Abdul Rani, Nor Hamdan Bin Mohamad Yahaya, Yasuhiko Tabata, Yosuke Hiraoka, Ruszymah Binti Haji Idrus, and Ng Min Hwei. 2021. "Type II Collagen-Conjugated Mesenchymal Stem Cells Micromass for Articular Tissue Targeting" Biomedicines 9, no. 8: 880. https://doi.org/10.3390/biomedicines9080880