Standardized Method for the Assessment of Behavioral Responses of Zebrafish Larvae
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Animals and Husbandry
2.2. Experimental Protocol
2.3. Statistics
3. Results
3.1. Light–Dark Tests with 15-Min Intervals
3.2. Light–Dark Tests with 5-Min Intervals
4. Discussion
4.1. Fear and Anxiety in Fish
4.2. Light–Dark Stimulation Test of Zebrafish Larvae
4.3. Number of Light and Dark Stimuli Repetitions
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Conflicts of Interest
References
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Maeda, H.; Fukushima, N.; Hasumi, A. Standardized Method for the Assessment of Behavioral Responses of Zebrafish Larvae. Biomedicines 2021, 9, 884. https://doi.org/10.3390/biomedicines9080884
Maeda H, Fukushima N, Hasumi A. Standardized Method for the Assessment of Behavioral Responses of Zebrafish Larvae. Biomedicines. 2021; 9(8):884. https://doi.org/10.3390/biomedicines9080884
Chicago/Turabian StyleMaeda, Hideyuki, Noritoshi Fukushima, and Akihiro Hasumi. 2021. "Standardized Method for the Assessment of Behavioral Responses of Zebrafish Larvae" Biomedicines 9, no. 8: 884. https://doi.org/10.3390/biomedicines9080884