Effect of PACAP on Hypoxia-Induced Angiogenesis and Epithelial–Mesenchymal Transition in Glioblastoma
Abstract
:1. Introduction
2. Methods
2.1. Human Glioblastoma Samples and Cell Line
2.2. Treatments
2.3. ELISA
2.4. Wound Healing Assay
2.5. Conditioned Medium and Preparation and Tube Formation Assay
2.6. Western Blot Analysis
2.7. Immunolocalization
2.8. Statistical Analysis
3. Results
3.1. PACAP and PAC1R Expression in GBM Hypoxic Area
3.2. PACAP Effect on VEGF Production in GBM Cells Exposed to DFX-Induced Hypoxia
3.3. PACAP Reduces New Vessels Formation Induced by Conditioned Media from GBM Cell Cultures
3.4. PACAP Counteracts the Hypoxia Mimetic Condition-Induced EMT in U87MG Cells
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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U87MG Cell Line-Derived Conditioned Media | CM1 (Vehicle) Mean ± SEM | CM2 (PACAP) Mean ± SEM | CM3 (DFX) Mean ± SEM | CM4 (DFX + PACAP) Mean ± SEM |
---|---|---|---|---|
VEGF (pg/mL) | 6584 ± 9.85 | 6041 ± 5.77 **** | 7203 ± 8.54 **** | 7013 ± 11.55 ### |
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Maugeri, G.; D’Amico, A.G.; Saccone, S.; Federico, C.; Rasà, D.M.; Caltabiano, R.; Broggi, G.; Giunta, S.; Musumeci, G.; D’Agata, V. Effect of PACAP on Hypoxia-Induced Angiogenesis and Epithelial–Mesenchymal Transition in Glioblastoma. Biomedicines 2021, 9, 965. https://doi.org/10.3390/biomedicines9080965
Maugeri G, D’Amico AG, Saccone S, Federico C, Rasà DM, Caltabiano R, Broggi G, Giunta S, Musumeci G, D’Agata V. Effect of PACAP on Hypoxia-Induced Angiogenesis and Epithelial–Mesenchymal Transition in Glioblastoma. Biomedicines. 2021; 9(8):965. https://doi.org/10.3390/biomedicines9080965
Chicago/Turabian StyleMaugeri, Grazia, Agata Grazia D’Amico, Salvatore Saccone, Concetta Federico, Daniela Maria Rasà, Rosario Caltabiano, Giuseppe Broggi, Salvatore Giunta, Giuseppe Musumeci, and Velia D’Agata. 2021. "Effect of PACAP on Hypoxia-Induced Angiogenesis and Epithelial–Mesenchymal Transition in Glioblastoma" Biomedicines 9, no. 8: 965. https://doi.org/10.3390/biomedicines9080965
APA StyleMaugeri, G., D’Amico, A. G., Saccone, S., Federico, C., Rasà, D. M., Caltabiano, R., Broggi, G., Giunta, S., Musumeci, G., & D’Agata, V. (2021). Effect of PACAP on Hypoxia-Induced Angiogenesis and Epithelial–Mesenchymal Transition in Glioblastoma. Biomedicines, 9(8), 965. https://doi.org/10.3390/biomedicines9080965