Decreased MicroRNA-150 Exacerbates Neuronal Apoptosis in the Diabetic Retina
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Cell Culture
2.3. Lipofectamine Transfection
2.4. Terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling (TUNEL)
2.5. The 3-[4,5-Dimethylthiazol-2-yl]-2,5 Diphenyl Tetrazolium Bromide (MTT) Colorimetric Assay
2.6. Quantitative Real-Time RT-PCR (qPCR)
2.7. Western Blot
2.8. Immunofluorescent Staining (Retina and Cultured Cells)
2.9. Statistical Analysis
3. Results
3.1. MicroRNA-150 Knockout (miR-150−/−) Exacerbates Apoptosis in the Diabetic Retina
3.2. MicroRNA-150 Knockdown Exacerbates Palmitic Acid (PA)-Elicited Apoptosis in Cultured 661W Cells
3.3. ETS-Domain Transcription Factor 1 (Elk1) Is a Direct Target of miR-150 and Contributes to T2D-Induced Apoptosis in Photoreceptors
3.4. Treatment with PA Increases ELK1 and Nuclear pELK1T417, and Knocking Down miR-150 Upregulates ELK1 and Cytoplasmic pELK1T417 in 661W Cells
3.5. Knocking Down Elk1 Decreases PA-Elicited Increases in Total ELK1 and pELK1T417 but Does Not Alleviate PA-Elicited Apoptosis in 661W Cells
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yu, F.; Ko, M.L.; Ko, G.Y.-P. Decreased MicroRNA-150 Exacerbates Neuronal Apoptosis in the Diabetic Retina. Biomedicines 2021, 9, 1135. https://doi.org/10.3390/biomedicines9091135
Yu F, Ko ML, Ko GY-P. Decreased MicroRNA-150 Exacerbates Neuronal Apoptosis in the Diabetic Retina. Biomedicines. 2021; 9(9):1135. https://doi.org/10.3390/biomedicines9091135
Chicago/Turabian StyleYu, Fei, Michael L. Ko, and Gladys Y.-P. Ko. 2021. "Decreased MicroRNA-150 Exacerbates Neuronal Apoptosis in the Diabetic Retina" Biomedicines 9, no. 9: 1135. https://doi.org/10.3390/biomedicines9091135